Connexin-36 Gap Junctions Regulate In Vivo First- and Second-Phase Insulin Secretion Dynamics and Glucose Tolerance in the Conscious Mouse

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FIG. 1.
FIG. 1.

Phenotype of Cx36−/− mice. A: Intraperitoneal (i.p.) glucose tolerance test (IPGTT) on male Cx36+/+ (■) and Cx36−/− mice (◇), each 16 weeks of age, after 2 g/kg body weight (b.w.) i.p. glucose injection. n = 6 littermate mice in each group. B: IPGTT on female mice 16 weeks of age, as in A. n = 8 littermate mice in each group. C: Oral GTT on male mice 16 weeks of age after 2 g/kg b.w. oral gavage. n = 6 littermate mice in each group. D: Area under the curve (AUC) of the glucose excursion during IPGTT after i.p. injection of variable amounts of glucose. n = 9 age-matched mice studied in parallel in each group. E: Plasma insulin measurements in male mice aged 16 weeks before (0) and 30 min after 3 g/kg b.w. i.p. glucose injection (black bars, Cx36+/+ mice; white bars, Cx36−/− mice). n = 8 littermate mice in each group. F: Plasma glucagon measurements in male mice aged 16 weeks, before (0) and 30 min after i.p. glucose injection, as in D. n = 7 littermate mice in each group. G: Insulin tolerance test after 0.75 units/kg i.p. insulin injection (see arrow). n = 10 littermate mice in each group. *, significant difference (P < 0.05, two-tailed Student t test) at each time point comparing measurements in Cx36+/+ and Cx36−/− mice.

This Article

  1. Diabetes vol. 61 no. 7 1700-1707