Overexpression of Monocarboxylate Transporter-1 (Slc16a1) in Mouse Pancreatic β-Cells Leads to Relative Hyperinsulinism During Exercise

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FIG. 2.
FIG. 2.

Abnormal stimulation of insulin secretion by pyruvate from islets in vitro. Islets isolated from a DT mouse were isolated, divided, and cultured for 48 h in the presence (+Dox) or absence (−Dox) of 5 μg/mL doxycycline. Batches of six size-matched islets were incubated in Krebs buffer with 3 mmol/L glucose for 60 min and then transferred to various concentrations of glucose, pyruvate, or lactate in Krebs buffer for 30 min. Secreted insulin is presented as a percentage of total insulin content of islets. Data are presented as mean ± SEM (n = 3–6). Data for each induction state were analyzed by ANOVA with Dunnett multiple comparison test to compare each condition with 3 mmol/L glucose *P < 0.05; **P < 0.01.

This Article

  1. Diabetes vol. 61 no. 7 1719-1725