Adipose Tissue Overexpression of Vascular Endothelial Growth Factor Protects Against Diet-Induced Obesity and Insulin Resistance

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FIG. 1.
FIG. 1.

Adipose-specific overexpression of VEGF led to increased vessel density. A: Representative Northern blot of epididymal WAT, BAT, skeletal muscle, and liver from wild-type (Wt) and transgenic (Tg) mice specific for Vegfa. B: Vegfa164 expression was analyzed by quantitative real-time PCR in epididymal WAT (eWAT), retroperitoneal WAT (rWAT), subcutaneous WAT (scWAT), and BAT and was normalized by 36B4 expression. Data represent the mean ± SEM of at least seven animals per group. $P < 0.05 vs. Wt. $$P < 0.01 vs. Wt. C: VEGF immunohistochemistry in WAT and BAT. Figure shows representative images at original magnification ×200, with insets at higher magnification. D: Representative WAT and BAT Western blots blotted with an antibody against VEGF and showing a band of 45 KDa corresponding to VEGF. E: Serum VEGF levels were measured in serum samples by enzyme-linked immunosorbent assay. Data represent the mean ± SEM of at least seven animals per group. F: Representative macroscopic images of eWAT (left) and interscapular BAT (right). G: Images of WAT and BAT angiographies were taken after a tail-vein dextran-conjugated fluorescein injection (green) and in toto with phalloidin (red), by confocal microscopy (original magnification ×400). Angiographies represent an overlay of 20 images (WAT) or 30 images (BAT) separated by 1 μm. H: Transmission electron microscopy study of capillary ultrastructure in WAT and BAT (original magnification ×2,000). L, lipid droplets; V, blood vessels; *, edema. (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 61 no. 7 1801-1813