Inhibiting de novo ceramide accumulation in vivo normalizes endothelial dysfunction and hypertension in C57Bl/6 mice with
diet-induced obesity. Systolic (A), mean (B), and diastolic (C) arterial blood pressure during light and dark cycles. Data are averaged from 4- × 24-h periods for 10 CON-V, 10 HF-V, 9
CON-M, and 9 HF-M mice. D: EDR. E: EIR. F: NR-mediated vasocontraction. G: Receptor (R)-mediated vasocontraction. Data are from two femoral artery segments from 18 CON-V, 23 HF-V, 15 CON-M, and 17
HF-M mice. A–H: *P < 0.05 HF-V vs. all. G: #P < 0.05 HF-M vs. all. Results represent mean ± SEM. H: Representative immunoblot and densitometry of the ratio of p-eNOS at serine (S)1177 to total eNOS from aorta/iliac arterial
homogenates from 8 CON-V, 13 HF-V, 6 CON-M, and 8 HF-M mice. *P < 0.05 HF-V vs. all. V, vehicle; M, myriocin.