Insulin Rescues Impaired Spermatogenesis via the Hypothalamic-Pituitary-Gonadal Axis in Akita Diabetic Mice and Restores Male Fertility

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FIG. 1.
FIG. 1.

Testicular atrophy in the male diabetic Akita mouse model. Nine-week-old wild-type, Akita heterozygous, and Akita homozygous mice (A) and corresponding testis decrease in size (B). C: Plasma insulin decreases in Akita heterozygotes and Akita homozygotes (9-week-old mice; n = 6). D: Blood glucose levels in Akita mice. homo, homozygous; het, heterozygous; wt, wild type. E: Hematoxylin-eosin stain of testes section. Akita homozygous testes (9-week-old) lack mature sperm cells and display vacuolization and large multinucleated cells. F: TUNEL analysis on Akita homozygous testes shows a greater degree of DNA damage in the seminiferous tubules. G: Negative and positive controls. (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 61 no. 7 1869-1878