Insulin Rescues Impaired Spermatogenesis via the Hypothalamic-Pituitary-Gonadal Axis in Akita Diabetic Mice and Restores Male Fertility

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FIG. 6.
FIG. 6.

Exogenous insulin treatment rescues testicular phenotype after unilateral orchiectomy. A: Wild-type (WT) and Akita mice before surgery and removal of one testis and insulin pellet treatment (n = 7 experiments with at least three mice in each group). B: Mice after treatment with insulin for 8 weeks. wk, week. Note the regeneration of the seminiferous tubules as well as the presence of all germ cell populations (n = 3 experiments); histology and testes images represent the remaining testis from the same mouse after removal of the first testis and subsequent insulin treatment. C: Mouse testis weight increases after insulin treatment (pre–insulin treatment, n = 7 experiments; post–insulin treatment, n = 3 experiments; *P < 0.01). D: CASA parameters normalize after treatment (n = 3 experiments). (A high-quality digital representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 61 no. 7 1869-1878