What Do Magnetic Resonance–Based Measurements of Pi→ATP Flux Tell Us About Skeletal Muscle Metabolism?
Magnetic resonance spectroscopy (MRS) methods offer a potentially valuable window into cellular metabolism. Measurement of flux between inorganic phosphate (Pi) and ATP using 31P MRS magnetization transfer has been used in resting muscle to assess what is claimed to be mitochondrial ATP synthesis and has been particularly popular in the study of insulin effects and insulin resistance. However, the measured Pi→ATP flux in resting skeletal muscle is far higher than the true rate of oxidative ATP synthesis, being dominated by a glycolytically mediated Pi↔ATP exchange reaction that is unrelated to mitochondrial function. Furthermore, even if measured accurately, the ATP production rate in resting muscle has no simple relationship to mitochondrial capacity as measured either ex vivo or in vivo. We summarize the published measurements of Pi→ATP flux, concentrating on work relevant to diabetes and insulin, relate it to current understanding of the physiology of mitochondrial ATP synthesis and glycolytic Pi↔ATP exchange, and discuss some possible implications of recently reported correlations between Pi→ATP flux and other physiological measures.
Magnetic resonance spectroscopy (MRS) methods offer a window on metabolism in vivo and can yield dynamic information in three main ways: firstly, from the kinetics of changes in metabolite concentrations [examples in muscle include 31P MRS measurements of postexercise phosphocreatine (PCr) resynthesis to probe mitochondrial ATP synthesis (1–3) and of PCr breakdown in ischemia to measure resting ATP turnover (2,4–11)]; secondly, using exogenous tracers [for example, the use of 13C MRS measurements of label transfer from infused [2-13C]acetate to muscle [4-13C]glutamate to estimate tricarboxylic acid cycle (TCAC) rate (12–16)]; and thirdly, measurements of unidirectional reaction rates by magnetization transfer (MT). Like isotope-labeling methods, MT has the advantage of being applicable to resting muscle. However, its interpretation is problematic. In this study, we discuss 31P MRS MT measurements of flux between inorganic phosphate (Pi) and ATP. Since the first report in 1989 by one of us (K.M.B.) of its application in working rat leg muscle (17), this has been applied to resting muscle to assess what is variously described as mitochondrial F1F0 ATPase activity, ATP synthase flux, unidirectional ATP production, or simply mitochondrial function (3,12,14–16,18–30). It has been used to study insulin effects (19,22,25,28,31,32) and insulin resistance (3,12,16,19–21,23–25,28,30,31), training (13,30), mitochondrial biogenesis (22,33), thyroid hormone pathophysiology (14,15,34), acromegaly (29), and burn injury (26,27) and has been combined with 13C MRS measurements of TCAC rate in an effort to quantify changes in mitochondrial coupling (13–15,33–36) [i.e., P:O, the ratio of ATP generated to oxygen consumed (37,38)].
As emphasized by one of us (G.J.K.) (39) and in a recent review (40) and commentary (41), the Pi→ATP flux measured by MT in resting skeletal muscle is far higher than estimates of oxidative ATP synthesis by other means, because [as discussed in the original article (17) and previously (42)], it is dominated by a glycolytically mediated Pi↔ATP exchange that is unrelated to mitochondrial ATP synthesis. Furthermore (39), even if measured accurately, the resting ATP turnover rate has no simple relationship to mitochondrial capacity as measured either in biopsy samples or in vivo from O2 consumption during maximal exercise (30) or indirectly from 31P MRS measurements of PCr recovery after submaximal exercise (43). Although both these points are becoming accepted (2,32), debate continues about the extent to which the measured Pi→ATP flux can be used as a measure of oxidative ATP production.
In this article, we summarize the quantitative evidence, concentrating on work relevant to diabetes and insulin, and relate it to the physiology of mitochondrial ATP synthesis and glycolytic Pi–ATP exchange. We show that the 1989 article (17) provides no warrant for the application of the Pi→ATP flux measurement to resting muscle and discuss some more general aspects of the interpretation of MT measurements and their relationship to metabolic fluxes. We point out how little is actually known about the changes in energy metabolism in insulin-stimulated and insulin-resistant muscle that this technique has been used to probe. We discuss a recent study (2) that addresses directly the relationship between Pi→ATP flux and MR-based measures of mitochondrial function and ATP turnover in normal muscle, and argue that (although interesting and surprising) the findings do not rehabilitate 31P MR MT measurements of Pi→ATP flux as a probe of mitochondrial metabolism in resting muscle.
THE QUANTITATIVE PROBLEM WITH Pi→ATP FLUX MEASUREMENTS
That the Pi→ATP flux far exceeds oxidative ATP turnover in resting skeletal muscle is easily appreciated visually. Figure 1A (which expands on Fig. 1A in Ref. 39) summarizes the published resting Pi→ATP fluxes in healthy adult human muscle (each data point being the mean from one study) in comparison with oxidative ATP synthesis rates derived from published 13C MRS estimates of TCAC rate and with a representative selection of resting ATP turnover measurements by three other methods (see figure legend). Although the 13C MRS values appear somewhat high (39,40), most obvious is the order-of-magnitude difference between the Pi→ATP flux and the rest.
INTERPRETATIONS OF EXPERIMENTS ON WORKING MUSCLE
Although the 1989 article (17) is often cited in support of the MT measurement in resting muscle (18,21,26–28,34), its message has evidently been misunderstood. In their recent review, From and Ugurbil (40) critically evaluated the analysis in the 1989 article and suggested that although its authors were aware of the problem with Pi↔ATP exchange catalyzed by the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 18.104.22.168) and phosphoglycerate kinase (PGK) (EC 22.214.171.124), “they concluded that the observed work-associated increase of measured Pi-ATP rate probably did not contain a major GAPDH/PGK component” (40). However, this is not so. Their comparison with published O2 consumption data (40) is essentially the same as that from which Brindle et al. (17) concluded that “… a contribution from the glycolytic enzymes cannot altogether be ruled out. For example, the oxygen consumption at rest in the perfused hind limb [….] corresponds to an ATP turnover rate […] which is at the lower end of the range of fluxes measured with a 10-ms stimulation pulse at a frequency of 0.5 Hz.” The 1989 article went on to point out that working muscle is different. During a 70-ms tetanus at 1 Hz, “the similarity of [the Pi→ATP flux] to the ATP turnover rate calculated from O2 consumption measurements in the perfused hind limb indicates that the contribution of a glycolytic exchange reaction […] is relatively small” (17). From and Ugurbil (40) largely agree with this analysis, although they suggest subtracting the resting rate from the fluxes measured in working muscle to get the true mitochondrial Pi→ATP flux, which Brindle et al. (17) declined to do because the glycolytic Pi↔ATP exchange might well change with work (44). Brindle et al. (17) were therefore correct to conclude that the GAPDH/PGK exchange likely makes only a relatively small contribution to the measured Pi→ATP flux in working muscle. The problem has arisen from the use of this analysis in resting muscle (40), first by Jucker et al. (34), who, citing Brindle et al. (17) as a method reference, combined 31P MRS MT with 13C MRS measures of TCAC rate to try to infer changes in mitochondrial coupling. This article (34) has formed the basis for subsequent MT studies that have claimed to detect mitochondrial dysfunction in skeletal muscle in insulin-resistant and other states, some (12,14,15,22,23,26–28,33,35,36,45) citing it directly as a method reference, and others (16,18–21,32) citing other articles (14,35) that do so.
Pi→ATP FLUX MEASUREMENTS VERSUS OTHER MEASURES
Much of the interest in resting muscle Pi→ATP flux has been in the differences in high-insulin and insulin-resistant states relative to control (3,12,16,19–21,23–25,28,30,31), in the acute response to insulin (19,22,25,28,31,32), and in correlations with other measurements (18,23,28,45,46). Figure 2 summarizes the insulin and insulin-resistance effects in human muscle and compares them with published resting ATP turnover data obtained by two other methods, 13C MRS measurements of TCAC rate and arteriovenous difference (AVD) measurements of O2 consumption.
Effects of insulin and insulin resistance.
Figure 2A summarizes how elevation of insulin (typically by euglycemic-hyperinsulinemic clamp) increases Pi→ATP flux (23,25,28,32), the effect being reduced or absent in insulin-resistant states such as lipid infusion (45), high-fat diet (18), type 2 diabetes (23), mitochondrial diabetes (25), and longstanding type 1 diabetes (28). Also shown, for comparison, are reported effects on the (much lower) estimates of oxidative ATP synthesis from AVD O2 consumption measurements: insulin stimulates this significantly in some (47–49) but not all (50,51) reports. As the euglycemic-hyperinsulinemic clamp results in a two- to sixfold increase in glucose oxidation and near-complete suppression of fat oxidation (47–51), the overall effect on oxidative ATP synthesis will depend (in an arithmetical sense) on the balance between these two opposing effects, about which little is known. Figure 2B also summarizes how, compared with controls, Pi→ATP flux in resting muscle is reduced in some (16,20,23–25), but not all (1,3,28,30) studies of insulin-resistant states. Also shown, for comparison, is the decreased oxidative ATP synthesis rate inferred from 13C MRS studies of TCAC rate in insulin-resistant first-degree relatives of type 2 diabetic patients (12) and the elderly (16), and the oxidative ATP synthesis rates estimated from AVD O2 consumption measurements in two studies of type 2 diabetic patients, one showing a significant increase relative to control (49) and one not (50). Again, how this abnormality in oxidative ATP synthesis might relate to the opposing effects of muscle insulin resistance on glucose and fat oxidation (49) is poorly understood.
Some correlations with Pi→ATP flux measurements.
Interest in the MT measurement has been stimulated by reported correlations with measures of insulin sensitivity and insulin effect. Pi→ATP flux correlates with glucose infusion rate in the euglycemic-hyperinsulinemic clamp across test and control groups in studies of fat-fed rats (18) and of lipid infusion in healthy humans (45) and type 2 diabetic patients and control subjects (23,28) [in whom it also correlates with glycogen synthesis rates (31)]. To interpret these findings, we need to consider the biochemistry of the Pi→ATP flux.
COMPONENTS OF Pi→ATP FLUX
The reason why the MT measurement of Pi→ATP flux is so high, as discussed in the 1989 article (17) as well as recently (40), is that the Pi→ATP flux catalyzed by the mitochondrial ATP synthase in resting muscle is dwarfed by the Pi↔ATP exchange catalyzed by the glycolytic enzymes GAPDH and PGK (44). As the net glycolytic flux through GAPDH/PGK is three orders of magnitude smaller than the exchange flux [being ∼10–50 μmol/L/min, increasing three- to eightfold in hyperinsulinemic-euglycemic clamp (47–51)], the GAPDH/PGK reactions are always near to equilibrium.
The Pi→ATP flux has commonly been expressed relative to a control group (16) or a basal state (32) or to TCAC rate measured by 13C MRS (16). However, because we know little about what controls the much larger glycolytic component, it definitely cannot be assumed that a relative decrease in Pi→ATP flux represents, even approximately, the same relative decrease in net oxidative ATP synthesis (which could change by a completely different amount or direction) nor that changes in absolute flux above basal (19) be taken as a guide to the increment in net oxidative ATP synthesis.
Pi→ATP FLUX AND CYTOSOLIC Pi CONCENTRATION
The MT experiment (17,40,44) gives the pseudo–first-order rate constant for flux between Pi and ATP (the absolute rate divided by the Pi concentration), which has no physiological meaning. This rate constant (usually expressed in s−1) must be multiplied by [Pi] (usually obtained from the Pi/ATP peak intensity ratio in a suitable spectrum, and a value of [ATP] assumed or measured by other means) in order to give the physiologically relevant flux (in this study, expressed as mmol/L/min). Cytosolic [Pi] and the exchange rate constant are reported separately (or can be inferred) in only a few MT studies in rat (35,52), mouse (26,27,36), and human muscle (1,2,13,28,32,45) [although relative changes in [Pi] are given in Petersen et al. (19) and can be estimated in multiple studies (20,22,29)]. Figure 1B summarizes how, for studies of healthy human muscle in which [Pi] is reported, relatively little between-study variation in Pi→ATP fluxes is explained by variation in [Pi], there being considerable between-study variation in the rate constant (Fig. 1C). [By contrast, Pi→ATP fluxes in the five published studies of mouse muscle (22,26,27,36,53) span a 10-fold range, and in the four studies in which this is reported, differences in [Pi] account for most of this variation.] Figure 1B shows two studies in which increased Pi→ATP flux in insulin infusion (19) and decreased Pi→ATP flux in insulin-resistant relatives of type 2 diabetic patients (20) is explained by altered cell [Pi], in the sense that the rate constant does not change (Fig. 1C). There are three reports in which Pi→ATP flux is dissociated from changes in [Pi]: slightly in treated acromegaly (29) and strikingly in experimental burn injury (26,27). This distinction is not usually reported.
The glycolytic component of the measured Pi→ATP flux depends on the activities of the two enzymes and the concentrations of their substrates and products, viz. NAD+ and NADH, Pi, ADP, ATP, glyceraldehyde 3-phosphate, 1,3-biphophosphoglycerate, and 3-phosphoglycerate (44), which we can only measure to a limited extent in vivo. Apart from [Pi], 31P MRS can monitor [ADP] (calculated usually from pH and [PCr], assuming literature values for [ATP] and total creatine concentrations), but this is not reported in the cited experiments. PCr concentration (measured as PCr/ATP) is unchanged in insulin infusion (19,54), but insulin increases cytosolic pH (54), which will tend to increase [ADP], and stimulates Na+-linked creatine accumulation (55), neither of which would necessarily change [ADP] (see the discussion of mitochondrial feedback control below). We have seen that there is little evidence of a causal influence of [Pi] on the measured Pi→ATP flux in resting human muscle unstimulated by insulin; however, in the absence of additive or countervailing effects of changes in unmeasured metabolites, the rise in [Pi] in insulin stimulation (Fig. 1B) (19,28) might increase the glycolytic exchange flux (44).
RESTING ATP TURNOVER AND MITOCHONDRIAL FUNCTION
From and Ugurbil (40), among others (56), note that reduction in maximum oxidative capacity should not affect the much lower rates of ATP supply in resting muscle. If a consistent closed-loop feedback relationship controls oxidative ATP synthesis across the whole dynamic range, and if resting ATP demand does not change, then a decrease in the mitochondrial capacity [which in 31P MRS terms is the notional maximum rate of oxidative ATP synthesis at maximum values of the error signal (43)] should mean an increase in the resting error signal (57)—for example, [ADP]. This exemplifies the general principle of metabolic control analysis that inhibition of an enzyme will tend to increase its substrate (upstream) concentrations; whether this results in a decrease in steady-state flux through the whole pathway depends on its regulatory structure (58); this is captured quantitatively by the flux control coefficient, which is related to the elasticity coefficients describing how concentrations of substrates and products affect the activities of individual pathway enzymes (58). In resting skeletal muscle the flux control coefficient of mitochondrial capacity is very low, control instead residing mainly in ATP demand. Even quite large changes in mitochondrial capacity should therefore have a negligible effect on resting ATP turnover.
CORRELATIONS WITH ATP TURNOVER RATE AND MITOCHONDRIAL CAPACITY
A recent study (2) has directly addressed some of these issues, finding that Pi→ATP flux correlates across healthy subjects with an ischemic measure of ATP turnover and also with PCr recovery-based measures of mitochondrial capacity. This might be thought to be in favor of MT measurements of Pi→ATP flux as a physiologically useful probe of muscle energy metabolism, despite the problems discussed above. However, the scale of the quantitative discrepancy undermines this. Consider the correlation with ATP turnover rate. Figure 3 shows individual data points taken from Schmid et al. (2) and the regression line through them (extrapolated for illustrative purposes); as this intercepts near the origin, the massively dominant nonoxidative component seems to scale with oxidative ATP synthesis rate, presumably because both measurements correlate with an underlying variable, which is so far unknown, For comparison, Fig. 3 shows the only other report (16) in which Pi→ATP flux measurements can be compared with a parallel measurement of oxidative ATP turnover (in this case, from TCAC rate by 13C MRS): mean data points are shown for healthy adults and elderly, insulin-resistant subjects (16), who show a low value by both MT and 31C MRS methods. This has been interpreted as showing reduced basal ATP turnover and normal mitochondrial coupling (16), but it seems safer to regard it as showing a low TCAC rate, with a so far unexplained decrease in the glycolytic component of Pi↔ATP exchange.
The interesting between-subjects correlation of Pi→ATP flux with measures of mitochondrial capacity was suggested (2) to reflect fiber-type differences. However, there appears to be no independent evidence that more oxidative fibers have greater basal ATP turnover.
MUSCLE MITOCHONDRIAL ABNORMALITY IN TYPE 2 DIABETES?
We will not discuss the wider evidence about mitochondrial dysfunction in type 2 diabetes in detail. From and Ugurbil (40) summarize some evidence against this, and numerous reviews have been published (e.g., Refs. 56,59,60). However, if (as we argue) MT does not tell us anything about mitochondrial function, it is of interest to ask what information other MR-based methods might be able to provide.
Probing mitochondrial function in vivo.
There are three main ways to assess muscle mitochondrial capacity: firstly, ex vivo (biopsy) measurements of mitochondrial number, mitochondrial enzyme and respiratory chain component content (61), and maximal respiration or ATP production rates in isolated mitochondria (61). Results from diabetic muscle are reviewed in Holloszy (56) and Pagel-Langenickel et al. (59). Secondly, in vivo measurements of oxygen consumption during maximal exercise (Vo2max), which test the integrated cardiorespiratory/vascular/muscular response (30), whereas measurements made by arteriovenous sampling can be specific to particular muscles. (Some results in diabetes are reviewed in Refs. 56,59.) Thirdly, MR techniques can provide reasonably well-tolerated noninvasive measurements of muscle in vivo. However, measurements of resting ATP turnover (even if accurate) cannot tell us about the capacity for oxidative ATP synthesis, and practical considerations rule out maximal exercise in the MR scanner. 31P MRS measurements of PCr recovery kinetics after submaximal exercise, however, can yield information about mitochondrial capacity in that this reflects a number of physiological and pathophysiological mechanisms, including mitochondrial number, content of oxidative enzymes or other mitochondrial components, and vascular supply of substrates and oxygen (43).
Evidence of mitochondrial dysfunction from postexercise PCr recovery kinetics.
Evidence of mitochondrial dysfunction, relative to appropriate controls, has been reported in type 2 diabetic patients in some studies (62–65) but not others (3,66), and even when group differences are observed, correlation with insulin resistance may be poor (65). However, a similar defect has also been reported in primary congenital insulin resistance (1) and congenital lipodystrophy (67), suggesting that muscle mitochondrial dysfunction can be a response to insulin resistance, as opposed to a cause. 31P MRS measures of mitochondrial function correlate with ex vivo measurements of muscle mitochondrial function and whole-body measures of aerobic fitness in type 2 diabetic patients (65,68), similarly to healthy controls (65). Exercise training improves 31P MRS measures of mitochondrial function (as well as insulin sensitivity) in some studies (64) but not others (3). A recent report that a short intensive exercise program improves maximal in vitro mitochondrial ATP production in healthy controls and the offspring of mothers with diabetes, but insulin sensitivity only in the former (61), argues against any simple link between mitochondrial function and insulin sensitivity.
Mitochondrial dysfunction and the pathophysiology of insulin resistance.
A popular hypothesis is that impaired lipid metabolism is associated with accumulation of intermediates (notably diacylglycerol) that interfere with postreceptor insulin signaling (e.g., Refs. 69,70). Furthermore, a feature of many insulin-resistant states is increased intramyocellular triglyceride (measurable by 1H MRS) (69), which is commonly attributed to impairment of fat catabolism. Neither postulate requires fat oxidation rate to be low, as it may be that higher intermediate concentrations overcome the effects of the defect in overall pathway flux. However, whether the enzyme defects that might be responsible for diacylglycerol and whether triglyceride accumulation would lead to decreases in mitochondrial capacity and function in vivo and ex vivo is not clear (see addendum for information on a recently published article that addresses this question). For mitochondrial oxygen consumption or ATP production ex vivo, the answer will depend on where the hypothesized defects are and the experimental conditions (e.g., nature of oxidizable substrate). 31P MRS measurements of postexercise PCr recovery are also complicated by questions of fuel selection: there are no good data on how much postexercise PCr recovery is driven by oxidation of fat after the relatively short exercise typical in 31P MRS experiments.
SUMMARY: THE INTERPRETATION OF MT EXPERIMENTS
The underlying problem with the interpretation of MT experiments has been that many have assumed that the method is measuring net chemical flux, whereas the experiment is formally equivalent to an isotope-exchange experiment (42,71). The relationship between the flux measured by MT and the metabolically relevant net chemical flux will depend on the enzyme’s mechanism (i.e., the order of substrate binding). For example, in the reaction catalyzed by creatine kinase, which has a random order equilibrium mechanism with rate-limiting interconversion of the ternary complexes (E.MgADP.PCr and E.MgATP.Cr), it is reasonable to equate the PCr→ATP flux measured by MT with overall flux through the reaction (71,72). However, at low ADP concentrations in vitro at pH 7.0, the ATP↔ADP exchange fluxes measured by MT are only half of the PCr↔ATP exchange fluxes, which are, however, similar to the isotope exchange fluxes of the 14C label between ATP and ADP and of the 15N label between Cr and PCr (71), as expected for this reaction mechanism. In other words, the MT experiment underestimates the creatine kinase-catalyzed ATP↔ADP exchange flux. This might be due to loss of magnetization in an on-enzyme intermediate, although this is considered theoretically unlikely (71). This discrepancy has received renewed attention with the recent demonstration that the ADP→ATP flux measured by MT in muscle can be explained by a transferred nuclear Overhauser effect between free and bound ATP, with only a very small contribution from chemical exchange between ATP and ADP (53). The authors of this study suggest that a large pool of ADP in muscle is bound to a large macromolecular assembly (e.g., actin), in which it is difficult to saturate the magnetization [see Brindle (42) for a discussion of this issue] and that this leads to underestimation of the ATP↔ADP exchange flux. However, if this exchange proceeds via a pool of free ADP, then this should be saturable and the exchange measurable, although if the very pool is very small and turning over very rapidly, then again it may be difficult to saturate (71). Balaban and Koretsky (41) have discussed recently the effects of metabolic exchange involving small pools of metabolites. Alternative explanations are direct transfer of the bound ADP to creatine kinase or possibly, more likely, a change in the enzyme mechanism in vivo such that dissociation of ADP from the enzyme becomes very slow, although still allowing rapid exchange between the ternary complexes and thus between ATP and PCr (71,72).
To summarize, in skeletal muscle at rest, the Pi→ATP flux measured by 31P MRS magnetization transfer is many times larger than the net rate of oxidative ATP synthesis (Fig. 1A). Thus, 31P MRS MT measurements of mitochondrial ATP turnover in skeletal muscle can only be made reliably in working muscle, where the GAPDH/PGK-catalyzed exchange is a smaller fraction of the total measured flux. This point was made in the 1989 article by Brindle et al. (17), which is often cited in support of the resting muscle measurement. The 2000 article by Jucker et al. (34), which has been cited as a method reference by many subsequent articles, is fundamentally flawed due to its failure to consider adequately the implications of this substantial GAPDH/PGK exchange in resting muscle. Even if the Pi→ATP flux measured by MT were a reliable measure of oxidative ATP synthesis in resting muscle, this would not be any guide to mitochondrial capacity. The Pi→ATP flux is increased by insulin and tends to be lower in insulin-resistant (IR) states than controls. There is some evidence from other methods (Fig. 2) that oxidative synthesis rate is increased by insulin (the net result of an opposing increase in glucose oxidation and decrease in fat oxidation) and that it is decreased in IR states. However, the apparent congruence between these relative changes may simply be an epiphenomenon.
A recently published review addresses the question of how products of lipid oxidation might impair insulin action in muscle: Watt MJ, Hoy AJ. Lipid metabolism in skeletal muscle: generation of adaptive and maladaptive intracellular signals for cellular function. Am J Physiol Endocrinol Metab 2012;302:E1315–1328.
No potential conflicts of interest relevant to this article were reported.
G.J.K. and K.M.B. drafted parts of the text, and both edited the final manuscript. G.J.K. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
- Received December 8, 2011.
- Accepted March 30, 2012.
- © 2012 by the American Diabetes Association.
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