Bcl-2 and Bcl-xL Suppress Glucose Signaling in Pancreatic β-Cells

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FIG. 2.
FIG. 2.

Bcl-2/Bcl-xL inhibition triggers cytosolic Ca2+ fluctuations. A and B: Cytosolic Ca2+ responses of groups of mouse islet cells exposed to Bcl-2/Bcl-xL inhibitors C6 and YC137 in the presence of 3 mmol/L glucose. C and D: Representative cytosolic Ca2+ responses to C6 in human islet cells (n = 66 cells from three islet preparations) and MIN6 β-cells (n = 34 cells). E: Quantification of the percentage of baseline quiescent mouse islet cells that responded within 30 min to various doses of C6 in the presence of either 3 or 0 mmol/L glucose (n = 3–6 for each condition; *P < 0.05 vs. control in 3 mmol/L glucose, #P < 0.05 vs. 40 μmol/L C6 in 3 mmol/L glucose; n.s., not significant). F: Average cytosolic Ca2+ responses of intact pancreatic islets stimulated with 15 mmol/L glucose in the presence or absence of 80 μmol/L C6. Shaded hanging bars represent SEM (n = 12, n = 10). (A high-quality color representation of this figure is available in the online issue.)

This Article

  1. Diabetes vol. 62 no. 1 170-182