Bcl-2 and Bcl-xL Suppress Glucose Signaling in Pancreatic β-Cells
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Differential subcellular distribution of Bcl-2 and Bcl-xL in β-cells. A and B: Representative images of MIN6 cells expressing GFP-tagged Bcl-2 and YFP-tagged Bcl-xL and loaded with 100 nmol/L MitoTracker Red. C: MIN6 cell coexpressing Bcl-xL:YFP and ER-targeted monomeric red fluorescent protein (mRFP). D: Pearson correlation coefficient (coeff.) quantifying colocalization of Bcl-xL:YFP with mitochondrial dsRed (n = 6) or ER mRFP (n = 5) and Bcl-2:GFP with ER mRFP (n = 5) in MIN6 β-cells (*P < 0.05, **P < 0.001). E: Western blots for endogenous Bcl-2 and Bcl-xL in fractions of MIN6 β-cells (n = 3). Cytochrome c oxidase (Cox-IV) indicates the mitochondrial fraction. (A high-quality digital representation of this
figure is available in the online issue.)