Impairments in Site-Specific AS160 Phosphorylation and Effects of Exercise Training
- Leslie A. Consitt1⇑,
- Jessica Van Meter2,
- Christopher A. Newton3,
- David N. Collier4,
- Moahad S. Dar5,
- Jørgen F.P. Wojtaszewski6,
- Jonas T. Treebak7,
- Charles J. Tanner2,8 and
- Joseph A. Houmard2,8
- 1Department of Biomedical Sciences, Ohio Musculoskeletal and Neurological Institute, Diabetes Institute, Ohio University, Athens, Ohio
- 2Department of Kinesiology, Human Performance Laboratory, East Carolina University, Greenville, North Carolina
- 3Division of Endocrinology, Metabolism and Lipids, Department of Internal Medicine, Emory University, Atlanta, Georgia
- 4Department of Pediatrics, Brody School of Medicine at East Carolina University, Greenville, North Carolina
- 5Section of Endocrinology & Metabolism, Department of Internal Medicine, Brody School of Medicine at East Carolina University, Greenville, North Carolina
- 6Molecular Physiology Group, The August Krogh Centre, Department of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
- 7Novo Nordisk Foundation Center for Basic Metabolic Research, Section on Integrative Physiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
- 8East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina.
- Corresponding author: Leslie A. Consitt, .
The purpose of this study was to determine if site-specific phosphorylation at the level of Akt substrate of 160 kDa (AS160) is altered in skeletal muscle from sedentary humans across a wide range of the adult life span (18–84 years of age) and if endurance- and/or strength-oriented exercise training could rescue decrements in insulin action and skeletal muscle AS160 phosphorylation. A euglycemic-hyperinsulinemic clamp and skeletal muscle biopsies were performed in 73 individuals encompassing a wide age range (18–84 years of age), and insulin-stimulated AS160 phosphorylation was determined. Decrements in whole-body insulin action were associated with impairments in insulin-induced phosphorylation of skeletal muscle AS160 on sites Ser-588, Thr-642, Ser-666, and phospho-Akt substrate, but not Ser-318 or Ser-751. Twelve weeks of endurance- or strength-oriented exercise training increased whole-body insulin action and reversed impairments in AS160 phosphorylation evident in insulin-resistant aged individuals. These findings suggest that a dampening of insulin-induced phosphorylation of AS160 on specific sites in skeletal muscle contributes to the insulin resistance evident in a sedentary aging population and that exercise training is an effective intervention for treating these impairments.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-0229/-/DC1.
- Received February 8, 2013.
- Accepted June 17, 2013.
- © 2013 by the American Diabetes Association.
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