Mouse Muscle As an Ectopic Permissive Site for Human Pancreatic Development

  1. Raphael Scharfmann1
  1. 1INSERM U845, Research Center Growth and Signalling, Faculté de Médecine Cochin, Université Paris Descartes, Paris, France
  2. 2Diabetes Research Laboratories, Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Oxford, U.K.
  3. 3Necker Enfants Malades University Hospital, Université Paris Descartes, Paris, France
  4. 4Biotechnology and Biotherapy Team, Université Pierre et Marie Curie-Paris 6, Biotechnology and Biotherapy Team, Centre de Recherche de l’Institut du Cerveau et de la Moelle épinière, UMRS 975, CNRS, UMR 7225, INSERM U975, Paris, France.
  1. Corresponding author: Raphael Scharfmann, raphael.scharfmann{at}inserm.fr.

Abstract

While sporadic human genetic studies have permitted some comparisons between rodent and human pancreatic development, the lack of a robust experimental system has not permitted detailed examination of human pancreatic development. We previously developed a xenograft model of immature human fetal pancreas grafted under the kidney capsule of immune-incompetent mice, which allowed the development of human pancreatic β-cells. Here, we compared the development of human and murine fetal pancreatic grafts either under skeletal muscle epimysium or under the renal capsule. We demonstrated that human pancreatic β-cell development occurs more slowly (weeks) than murine pancreas (days) both by differentiation of pancreatic progenitors and by proliferation of developing β-cells. The superficial location of the skeletal muscle graft and its easier access permitted in vivo lentivirus-mediated gene transfer with a green fluorescent protein-labeled construct under control of the insulin or elastase gene promoter, which targeted β-cells and nonendocrine cells, respectively. This model of engraftment under the skeletal muscle epimysium is a new approach for longitudinal studies, which allows localized manipulation to determine the regulation of human pancreatic development.

Footnotes

  • Received April 8, 2013.
  • Accepted June 26, 2013.

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  1. Diabetes vol. 62 no. 10 3479-3487
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