In This Issue of Diabetes

Edited by Helaine E. Resnick, PhD, MPH

Novel Tool for Studying the Pathophysiology of Obesity and Diabetes

Adeno-associated viral (AAV)–mediated engineering of adipose tissue may emerge as a valuable tool for the development of new therapies for both obesity and type 2 diabetes. Impaired activity or mass of brown adipose tissue (BAT) and white adipose tissue (WAT) are known contributors to obesity and type 2 diabetes in both animal models and humans. Although genetic engineering of adipose cells could shed light on the molecular mechanisms underlying obesity and diabetes, in vitro studies have encountered numerous technical challenges that have inhibited forward movement toward a better understanding of molecular mechanisms. Both cultured immortalized precursor cell lines and primary adipocytes may also differ in important ways from adipocytes in vivo, potentially limiting the application of in vitro findings. In the new work presented in this month’s issue of Diabetes, Jimenez et al. (p. 4012) examined AAV vectors of serotypes 1, 2, 4, 5, 6, 7, 8, and 9 in order to determine whether these vectors could transduce murine WAT and BAT in vivo. The investigators found that AAV8 and AAV9 vectors mediated long-term, efficient transduction of WAT and BAT in adult lean and obese-diabetic mice. These results were observed both with local and systemic administration. Importantly, the findings of a recent study that reported proinflammatory cytokines in the liver following intravenous administration of double-stranded AAV vectors in mice were not consistent with observations in the new report, which demonstrated no inflammation of the liver or adipose tissue following intravenous administration of single-stranded AAV8 or AAV9 vectors. The investigators highlight the application of their findings by suggesting that AAV vectors could be …

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This Article

  1. doi: 10.2337/db13-ti12 Diabetes vol. 62 no. 12 3961-3962
  1. Free via Open Access: OA