Peroxynitrite-Dependent Zinc Release and Inactivation of Guanosine 5′-Triphosphate Cyclohydrolase 1 Instigate Its Ubiquitination in Diabetes

  1. Ming-Hui Zou1,2,3
  1. 1Section of Molecular Medicine, Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  2. 2Hubei Province Key Laboratory on Cardiovascular, Cerebrovascular, and Metabolic Disorders, Hubei University of Science and Technology, Xianning, Hubei, China
  3. 3Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  1. Corresponding author: Ming-Hui Zou, ming-hui-zou{at}ouhsc.edu.

Abstract

Aberrant degradation of guanosine 5′-triphosphate cyclohydrolase 1 (GTPCH1) with consequent deficiency of tetrahydrobiopterin is considered the primary cause for endothelial dysfunction in diabetes. How GTPCH1 becomes susceptible to the degradation remains unknown. We hypothesized that oxidation and release of the zinc ion by peroxynitrite (ONOO), a potent oxidant generated by nitric oxide and superoxide anions, instigates GTPCH1 ubiquitination and degradation. Zinc contents, GTPCH1 ubiquitination, and GTPCH1 activity were assayed in purified GTPCH1, endothelial cells, and hearts from diabetic mice. Exogenous ONOO dose-dependently released zinc, inhibited its activity, and increased the ubiquitin binding affinity of GTPCH1 in vitro and in endothelial cells. Consistently, high glucose (30 mmol/L) inhibited GTPCH1 activity with increased ubiquitination, which was inhibited by antioxidants. Furthermore, mutation of the zinc-binding cysteine (141) (C141R or C141A) significantly reduced GTPCH1 activity and reduced its half-life but increased GTPCH1 ubiquitination, indicating an essential role of the zinc ion in maintaining the catalytic activity and stability of GTPCH1. Finally, GTPCH1 ubiquitination and degradation markedly increased in parallel with decreased GTPCH1 activity in the aortas and hearts of diabetic mice, both of which were attenuated by the inhibitors of ONOO in mice in vivo. Taken together, we conclude that ONOO releases zinc and inhibits GTPCH1, resulting in its ubiquitination and degradation of the enzyme.

An adequate supply of tetrahydrobiopterin (BH4) in the endothelium is critical for maintaining the “coupled” status of endothelial nitric oxide synthase (eNOS) in healthy subjects. In contrast, BH4 deficiency, which is widely found in diseased vessels, is considered responsible for eNOS uncoupling in diabetes (14) or hypertension (5,6). Guanosine 5′-triphosphate cyclohydrolase 1 (GTPCH1) is the first enzyme in the de novo biosynthetic pathway of BH4. GTPCH1 inhibition leads to a rapid decrease of BH4 and consequent eNOS uncoupling (7). GTPCH1 is regulated by several mechanisms, including transcription, posttranslational modifications (7), and association with the GTPCH feedback regulatory protein (GFRP), which inhibits GTPCH1 activity (8).

Zinc binds to a large number of proteins, including numerous metalloenzymes, structural proteins, and transcription factors (9). In the human GTPCH1 crystal structure, zinc binds to two cysteines and one histidine (C141-H144-C212) (10) and is reported to be important in maintaining GTPCH1 structure and function (10). Because zinc has the highest charge-to-atomic radius ratio of any element and maintains a partial cationic character (11,12), zinc ion is reported to react fast with anionic oxidants such as peroxynitrite (ONOO) and HOCl. For example, ONOO reacts rapidly with zinc-containing proteins such as eNOS, protein kinase C, and yeast alcohol dehydrogenase, because the zinc-thiolate cluster represents a selective target for ONOO (1114).

Recent evidence indicates that loss or inactivation of the GTPCH1 protein and the consequent BH4 deficiency causes eNOS uncoupling in diabetes (1,7,15). Although the increased proteasome activity in diabetic mice might be an important cause for GTPCH1 degradation (1), the mechanism that caused the preferential degradation of GTPCH1 by proteasome is yet unknown. Diabetes-induced GTPCH1 loss is likely not caused by transcription because there is no change in GTPCH1 mRNA levels in streptozotocin (STZ)-induced diabetic mice (1). Diabetes does not affect the association of GTPCH1 with GFRP, which impairs GTPCH1 activity (1). Therefore, the modification of GTPCH1 protein might explain accelerated destruction in diabetes. Here, we provide evidence that ONOO releases zinc, inhibits GTPCH1 activity, and increases GTPCH1 ubiquitination.

RESEARCH DESIGN AND METHODS

STZ-induced diabetes in mice.

C57BL/6J mice, aged 8–12 weeks, were obtained from the Jackson Laboratory (Bar Harbor, ME). Mice were housed in temperature-controlled cages with a 12-h light/dark cycle and given free access to water and food. Mice were divided into four groups and received an injection of STZ (50 mg/kg body weight daily) for 5 consecutive days to induce diabetes (16). Diabetes is defined as random blood glucose levels of >450 mg/dL for >2 weeks after injection. One additional group of STZ-treated mice was subsequently treated with 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (Tempo; in drinking water, 1 mmol/kg body weight) for 4 weeks. All animal experiments were approved by the Institutional Animal Care and Use Committee at the University of Oklahoma Health Sciences Center.

Cells.

Bovine aortic endothelial cells (BAECs), used between passages 3 and 10, were grown in endothelial basal medium (Lonza, Basel, Switzerland) supplemented with 2% FBS and 1% (volume for volume [v/v]) penicillin-streptomycin. Human embryonic kidney 293 (HEK293) cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 1% (v/v) penicillin-streptomycin and 10% (v/v) FCS.

Plasmids construction and mutagenesis.

Full-length human GTPCH1 cDNA (GCH1) was synthesized by PCR amplification. The amplified DNA with a COOH-terminal FLAG tag was directionally cloned into plasmid pCI-neo (Promega, Madison, WI) for mammalian protein expression. For Escherichia coli (BL21) protein expression, GCH1 was inserted into pGEX-4T-2 (Amersham Pharmacia Biotech). The zinc-deletion mutant of GTPCH1 (C141R) was generated using the Stratagene QuikChange site-directed mutagenesis kit. Plasmid pRK5-HA-ubiquitin (histidine [his] tag [HA]-Ub; Addgene ID 17608) was obtained from Addgene (Cambridge, MA). All constructed plasmids were sequenced entirely before use.

Expression and purification of recombinant GTPCH1 in bacteria and mammalian cells.

Glutathione S-transferase (GST)-tagged GTPCH1 or C141R were expressed in the E. coli BL21 (DE3). The cells were resuspended in lysis buffer (100 mmol/L Tris-HCl, pH 8.5, 100 mmol/L NaCl, 10% glycerol, 1% Triton X-100, and protease inhibitors [Calbiochem]) and then lysed by brief sonication. The GST fusion proteins were purified with GSH beads in accordance with the manufacturer’s protocol. For the expression of recombinant GTPCH1 in mammalian cells, FLAG-tagged wild-type (WT) or C141R GTPCH1 plasmids were transfected into HEK293 cells. The expressed FLAG-GTPCH1 or C141R proteins were purified using anti-FLAG resin (Sigma-Aldrich), according to the protocol provided by the manufacturer.

Detection of protein S-nitrosylation with the biotin-switch.

S-nitrosylated GTPCH1 was monitored by using the kits from Cayman Chemicals, according to the method provided by the supplier.

Assay of GTPCH1 activity.

GTPCH1 activity was measured as described previously (1,17). This assay was based on the quantification of d-erythro-neopterin by high-performance liquid chromatography (HPLC).

Statistical analysis.

Results are expressed as mean ± SD. All values are normalized to control, and the means of the control are calculated. Statistical significance for comparisons between two groups was calculated using the two-tailed paired Student t test. To assess comparisons between multiple groups, ANOVA followed by the Bonferroni procedure was performed using the GraphPad Prism 4 program (GraphPad Software, Inc., San Diego, CA). P < 0.05 was considered to be statistically significant.

Materials and other methods, including in vitro pull-down assays, enrichments of ubiquitinated proteins, determination of GTPCH1 half-life assays, and detection of superoxide anion, can be found in the Supplementary Data.

RESULTS

ONOO inhibits whereas NO activates GTPCH1 activity in vitro.

Bacterially expressed GTPCH1 was first purified as described in the research design and methods. Purified GTPCH1 was seen as a single band at 30 kDa in silver staining of the gel (Fig. 1A). To determine the effects of ONOO or NO on GTPCH1 activity, purified recombinant GTPCH1 proteins (0.5 μg) were exposed to chemically synthesized ONOO (0–100 μmol/L) or vehicle (0.1 mol/L NaOH). As shown in Fig. 1B and Supplementary Fig. 1, ONOO (1–100 μmol/L) inhibited GTPCH1 activity in a dose-dependent manner, causing an ∼70% reduction of GTPCH1 activity at the highest concentration (100 μmol/L). In contrast, exposure of GTPCH1 activity to DETA-NONOate (DETANO; 200 μmol/L) significantly increased GTPCH1 activity (Fig. 1C). Similarly, exposure of GTPCH1 to spermine NONOate (SPERNO; 1 or 5 mmol/L) activated the enzyme (data not shown).

FIG. 1.

ONOO impairs recombinant GTPCH1 activity but increases its ubiquitination associated with zinc release. A: Purification of recombinant FLAG-GTPCH1. The purity of FLAG-GTPCH1 was analyzed by SDS-PAGE using silver staining. B: Effect of ONOO on purified recombinant GTPCH1 activity. The enzyme activity of GTPCH1 was determined by HPLC. Data are shown as mean ± SD (n = 6). *P < 0.05 vs. control. C: Effect of NO on recombinant GTPCH1 activity. *P < 0.05 when compared to Vehicle. D: NO but not ONOO increases the S-nitrosylation of GTPCH1. E: ONOO stimulated zinc release from recombinant GTPCH1. Zinc was assayed as described in research design and methods and was expressed as percentage of maximal zinc release from GTPCH1 diluted in 7 mol/L guanidine HCl. n = 3. *P < 0.05 vs. control. F: The effect of the zinc chelator TPEN on GTPCH1 activity. n = 3. *P < 0.05 vs. control; #P < 0.05 vs. +TPEN 200 μmol/L. CTR (+), with biotin label; FT, flow through, after affinity purification; IB, immunoblot.

Nitric oxide but not ONOO increases the S-nitrosylation of GTPCH1 in vitro.

We next determined if ONOO inhibition on GTPCH1 was via S-nitrosylation of the enzyme. Recombinant FLAG-GTPCH1 were treated with vehicle or ONOO (100 μmol/L) and then applied for biotin-switch assay, a commonly used method for the detection of S-nitrosylation. As expected, S-nitrosylation of GTPCH1 was barely detectable in the basal condition and was not altered by 100 μmol/L of ONOO treatment (Fig. 1D). In addition, exposure of GTPCH1 to ONOO did not have any obvious effect on the S-nitrosylation of GTPCH1 with or without N-ethylmaleimide (NEM), an organic compound that irreversibly reacts thiols (Fig. 1D). In contrast, DETANO markedly increased the detection of S-nitrosylated GTPCH1 (Fig. 1D). Further, pretreatment of GTPCH1 with NEM ablated nitric oxide (NO)-increased S-nitrosylation of GTPCH1 (Fig. 1D). A similar effect was also observed with sperNO (data not shown). Taken together, NO but not ONOO increased the S-nitrosylation of GTPCH1.

ONOO releases zinc from GTPCH1.

Zinc is important for maintaining the structure and function of GTPCH1 (10). Because ONOO reacts fast with the positively charged zinc ion, resulting in its release from zinc-containing proteins (11), we next assayed whether or not ONOO released zinc from recombinant GTPCH1. As depicted in Fig. 1E, ONOO increased zinc release from GTPCH1 in a concentration-dependent manner. ONOO (50 μmol/L) caused ∼60% zinc release, whereas a higher concentration of ONOO (100 μmol/L) increased zinc release to ∼80% (Fig. 1E). In contrast, NO had no effects on zinc release from GTPCH1 (Fig. 1E).

Inhibition of GTPCH1 activity by zinc chelator TPEN in vitro.

To further support the concept that zinc release is responsible for ONOO-induced GTPCH1 inhibition, we next determined if chemical zinc chelation mimicked the effects of ONOO. To this end, recombinant GTPCH1 proteins were incubated with N,​N,​N',​N'-​tetrakis(2-​pyridinylmethyl)-​1,​2-​ethanediamine (TPEN), a potent zinc-chelating agent (11). Because TPEN was found to interfere with the GTPCH1 activity assay at concentrations ≥500 μmol/L, 40 and 200 μmol/L TPEN were used in this study. As shown in Fig. 1F, the addition of 40 or 200 μmol/L TPEN reduced GTPCH1 activity by 15 and 35%, respectively. Importantly, the inhibitory effects of TPEN (200 μmol/L) were abolished by the addition of 10 μmol/L zinc chlorite (Fig. 1F), indicating that the inhibitory effect of TPEN on GTPCH1 was likely due to zinc chelation.

ONOO increases GTPCH1 binding to ubiquitin in vitro.

Protein oxidation and the consequent conformational changes usually result in ubiquitination and degradation (18,19,20). As zinc is important not only for GTPCH1 activity but also in maintaining its structure (10), we reasoned that zinc release from GTPCH1 by ONOO might increase ubiquitin binding. To test this hypothesis, FLAG-GTPCH1 was expressed in HEK293 cells and purified using anti-FLAG resin. The purity of FLAG-GTPCH1 was analyzed by SDS-PAGE using silver staining (data not shown). Purified proteins were further identified by immunoblotting (Fig. 2A). To examine whether ONOO increased GTPCH1 ubiquitination, purified FLAG-GTPCH1 immobilized on anti-FLAG resin was first treated with ONOO (10–100 μmol/L) and then incubated with tetraubiquitin (Ub4). The Ub4 bound to FLAG-GTPCH1 (ONOO or vehicle treated) was further analyzed by immunoblot. As shown in Fig. 2B and D, the recombinant FLAG-GTPCH1 for each experimental group is equal, and ONOO did not significantly change the FLAG-GTPCH1 amount on anti-FLAG resin. FLAG-GTPCH1, but not the negative control (anti-FLAG resin only), directly interacts with Ub4. Importantly, ONOO increased the binding of FLAG-GTPCH1 to Ub4 in a dose-dependent manner (Fig. 2B and C). ONOO (100 μmol/L) increased GTPCH1 binding to Ub4 by approximately fourfold (Fig. 2C).

FIG. 2.

ONOO increases GTPCH1 ubiquitination. A: Purification and identification of FLAG-GTPCH1 proteins. FLAG-GTPCH1 plasmids were transfected to HEK293 for 2 days, and the proteins were analyzed by immunoblotting using anti-FLAG antibody or antibody specific for GTPCH1. B and C: Recombinant FLAG-GTPCH1 bound to anti-FLAG resin was treated with ONOO (10–100 μmol/L, separately) and incubated with Ub4. Bound proteins were detected by immunoblotting. D: Effect of zinc supplementation (zinc chloride, 10 μmol/L) on ONOO-induced GTPCH1 ubiquitination. E: Effects of NO and ONOO on GTPCH1 S-nitrosylation in purified recombinant GTPCH1. CTR (−), no biotin label; CTR (+) with biotin label; vehicle (NaOH) treated and biotin labeled; ONOO (100 μmol/L), DETANO 200 μmol/L; spermine NONOate (SPERNO) 5 or 1 mmol/L, and then applied for biotin-switch assay. F: Effects of NEM on NO- or ONOO-induced S-nitrosylation. The blot is representative of three to five blots from three to five individual experiments. IB, immunoblot; Ub, ubiquitin.

Zinc supplementation does not affect ONOO-induced GTPCH1 ubiquitination.

To test if zinc supplement prevented the effect of ONOO on GTPCH1, 10 μmol/L ZnCl2 was added prior to or after ONOO addition. As shown in Fig. 2D, zinc supplementation failed to reverse ONOO-induced GTPCH1 ubiquitination

NO but not ONOO increases the S-nitrosylation of GTPCH1 in intact cells.

GTPCH1-overexpressing HEK293 cells were exposed with DETANO or ONOO (100 μmol/L). As depicted in Fig. 2E, DETANO but not ONOO markedly increased the detection of S-nitrosylated GTPCH1. As expected, pretreatment with NEM ablated NO-increased S-nitrosylation of GTPCH1 (Fig. 2E). In contrast, ONOO did not have any obvious effect on the S-nitrosylation of GTPCH1 in intact cells (Fig. 2F).

Genetic zinc deletion from GTPCH1 affects its activity.

Because mutation of one of the amino acids in the GTPCH1 zinc binding site abolishes its zinc binding (21), we generated zinc-free C141R and C141A mutants. As shown in Fig. 3A, the zinc-free C141R and C141A mutants had barely detectable activity when compared with WT GTPCH1 (Fig. 3A). Although ONOO significantly inhibited GTPCH1 activity in WT, ONOO did not alter the activity of C141A and C141R GTPCH1 mutants (Fig. 3B).

FIG. 3.

Genetic deletion of zinc from GTPCH1 impairs its activity and accelerates GTPCH1 degradation and ubiquitination via direct ubiquitin binding. A: Enzyme activity of recombinant GTPCH1 and zinc-deleted GTPCH1 (C141A and C141R). FLAG-tagged WT or C141A or C141R GTPCH1 plasmids were constructed and expressed in HEK293 cells as described in research design and methods. Recombinant WT GTPCH1 or the C141A or C141R mutants were purified by anti-FLAG resin and subjected to activity assay by HPLC. #, n = 3. *P < 0.05 vs. control. B: Effects of ONOO on GTPCH1 activity of GTPCH1 WT, C141A, or C141R mutant. *P < 0.05 when compared to vehicle-treated WT. C: Representative blots of three independent experiments showing the protein stability of C141R. HEK293 cells were transfected with FLAG-C141R or -GTPCH1 plasmids. Twenty-four hours after transfection, cells were incubated with CHX (100 μg/mL), and, at the indicated times, cells were harvested, lysed, and analyzed for GTPCH1 levels by using an anti-FLAG antibody. In all cases, β-actin levels were measured as a loading control. D: C141R ubiquitination in cultured cells. HEK293 cells were cotransfected with FLAG-tagged WT or C141R GTPCH1, and HA-tagged ubiquitin plasmid or empty vector. Cell lysates were immunoprecipitated by anti-FLAG resin and immunoblotted using the indicated antibodies. E: Effects of C141R ubiquitination in vitro. Purified recombinant GST-GTPCH1 (WT) or GST-C141R proteins were incubated with Ub4. Bound ubiquitin chains were separated by SDS-PAGE and analyzed by immunoblotting. The blot shown is representative of three independent experiments. Data are shown as mean ± SD (n = 3). *P < 0.05 vs. control. NS, not significant; IB, immunoblot; IP, coimmunoprecipitation; Ub, ubiquitin.

The zinc-deletion GTPCH1 mutant (C141R) exhibits reduced half-life.

To determine the stability of C141R, FLAG-GCH1 or C141R was transfected into HEK293 cells. After the transfection for 48 h, the cells were treated with cycloheximide (CHX; 100 μg/mL). At the times indicated, cells were harvested to assay GTPCH1 levels. As shown in Fig. 3C, the half-life of C141R GTPCH1 was <1 h, whereas WT GTPCH1 exhibited no significant change within 12 h, suggesting that zinc deletion markedly reduces GTPCH1 stability.

Increased ubiquitination in cells overexpressing GTPCH1 C141R mutants.

We next determined whether zinc affects GTPCH1 ubiquitination. To this end, FLAG-GTPCH1 or C141R was transfected into HEK293 cells with or without the coexpression of HA-Ub. HA-Ub chains bound to FLAG-GTPCH1 or C141R were detected by coimmunoprecipitation assays using anti-FLAG resin. As shown in Fig. 3D, in the absence of HA-Ub, there was no detectable signal in the blot (Fig. 3D, lanes 1 and 3). When HA-Ub and FLAG-GTPCH1 were simultaneously overexpressed in HEK293 cells, HA-Ub chains were found in the immunoprecipitates of FLAG-GTPCH1 (Fig. 3D, lane 2), which indicates that GTPCH1 specifically associates with polyubiquitin chains. Importantly, genetic zinc deletion in GTPCH1 (FLAG-C141R) dramatically increased its association with HA-Ub chains when compared with the coexpression of HA-Ub and WT FLAG-GTPCH1 (Fig. 3D).

GTPCH1 C141R exhibits increased binding with Ub4 in vitro.

Next, we determined how zinc removal affects the GTPCH1/ubiquitin association. We assayed the affinity of purified GST-tagged WT GTPCH1 and the C141R mutant with Ub4 in pull-down assays in vitro. Ub4 was pulled down by GST-GTPCH1 but not GST beads (Fig. 3E). In addition, when compared with WT GST-GTPCH1, GST-C141R dramatically increased binding to Ub4 in vitro (Fig. 3E), suggesting that zinc deletion in GTPCH1 increased its degradation, likely via increased binding to ubiquitin chains.

ONOO inhibits GTPCH1 activity in intact endothelial cells.

We next determined the effects of ONOO on GTPCH1 in cultured BAECs. As depicted in Fig. 4A, exposure of BAECs to ONOO (50 μmol/L) for 30 min caused ∼60% inhibition of GTPCH1. Similarly, short exposure (1 h) of BAECs to 3-morpholinosydnonimine N-ethylcarbamide (SIN-1), an ONOO donor, lowered GTPCH1 activity by ∼50% (Fig. 4B).

FIG. 4.

ONOO inhibits the activity but increases the degradation of GTPCH1 in endothelial cells. A: The effect of ONOO on GTPCH1 activity in BAECs. Confluent BAECs were starved overnight and treated with ONOO (1, 10, or 50 μmol/L, separately) or equal volume of vehicle (0.1 mol/L NaOH) for 15 min in 0.1 mol/L HEPES buffer. HPLC was used to determine the GTPCH1 enzyme activity. n = 3. *P < 0.05 vs. control. B: BAECs were treated with SIN-1 for indicated time. n = 3. *P < 0.05 vs. control. C and D: Effect of ONOO on GTPCH1 stability. BAECs were treated with 50 μmol/L ONOO or vehicle for 15 min and then with CHX (100 μg/mL). Cells were harvested at the indicated time points in the presence of CHX, and the lysates were subjected to immunoblotting using anti-GTPCH1 and anti–β-actin antibodies. The blot shown is representative of three independent experiments.

ONOO reduces GTPCH1 stability in endothelial cells.

To determine whether ONOO affects the stability of GTPCH1, confluent endothelial cells were exposed to ONOO (50 μmol/L) or vehicle. After the treatment, CHX (100 µg/mL) was added to block de novo protein synthesis. The levels of GTPCH1 were unchanged in vehicle-treated BAECs during 4 h of incubation. However, the levels of GTPCH1 in ONOO-treated BAECs were only ∼20% of those in vehicle-treated cells (Fig. 4C). The half-life of GTPCH1 in ONOO-treated BAECs was reduced to ∼1.6 h (Fig. 4D).

ONOO destabilizes GTPCH1 via increased ubiquitination.

We next determined if the reduced GTPCH1 stability was due to the increased GTPCH1 ubiquitination. Ubiquitinated GTPCH1 was monitored in the presence or absence of MG132, a potent proteasome inhibitor. MG132 alone caused a slight but clear increase in ubiquitinated GTPCH1 (Fig. 5A). In the absence of MG132, ONOO (50 μmol/L) did not alter the level of ubiquitinated GTPCH1 (Fig. 5A). In the presence of MG132, ONOO (50 μmol/L) markedly increased ubiquitinated GTPCH1 compared with the level in vehicle-treated cells (approximately sixfold) (Fig. 5B). Importantly, ONOO-induced ubiquitination disappeared in the absence of MG132, a potent proteasome inhibitor (Fig. 5A).

FIG. 5.

ONOO increases GTPCH1 ubiquitination of intact endothelial cells. A and B: The effect of ONOO on GTPCH1 ubiquitination in cultured BAECs. Confluent BAECs were pretreated with the 26S proteasome inhibitor MG132 (0.5 μmol/L) or an equivalent volume of DMSO (control) for 30 min, and treated with ONOO (50 μmol/L) or vehicle in 0.1 mol/L HEPES buffer. BAEC lysates were incubated with ubiquitin interacting motif-agarose. GTPCH1 that was affinity precipitated with ubiquitin was analyzed by immunoblotting using anti-GTPCH1 and anti−β-actin antibodies. IB, immunoblot; Ub, ubiquitin. C and D: SIN-1 on GTPCH1 ubiquitination. BAECs were pretreated with MG132 or DMSO for 30 min and treated with 1 mmol/L SIN-1 (ONOO donor). The blot shown is representative of three independent experiments. NS, not significant. *P < 0.05 vs. control.

We also tested the effects of SIN-1 (1 mmol/L) on GTPCH1 ubiquitination. In the absence of MG132, SIN-1 did not alter the levels of ubiquitinated GTPCH1 when compared with those in vehicle-treated cells (Fig. 5C). MG132 alone slightly increased the levels of ubiquitinated GTPCH1 (approximately twofold) (Fig. 5D). In the presence of MG132, SIN-1 markedly increased the levels of ubiquitinated GTPCH1 (approximately sixfold) (Fig. 5D). Taken together, these data suggest that ONOO enhanced the GTPCH1 ubiquitination in endothelial cells, which caused its destruction and reduced half-life.

High glucose increased GTPCH1 ubiquitination in endothelial cells.

There is evidence that exposure of endothelial cells to high glucose enhances the levels of both O2· and ONOO, which increased proteasome activity and caused GTPCH1 degradation in human umbilical vein endothelial cells (HUVECs) (1). We first confirmed the high glucose effects on GTPCH1 protein level. High glucose (30 mmol/L), but not an osmotic glucose control (OG), dramatically reduced GTPCH1 protein levels in BAECs (Fig. 6A).

FIG. 6.

High glucose increases GTPCH1 ubiquitination and degradation. A: High glucose increases GTPCH1 degradation. Confluent BAECs were treated with normal-glucose (NG) medium (d-glucose, 5 mmol/L), osmotic glucose (OG; d-glucose 5 mmol/L, l-glucose 25 mmol/L), or high glucose (HG; d-glucose, 30 mmol/L), and the cells were harvested, lysed, and analyzed for GTPCH1 levels by immunoblotting. B and C: High glucose increases GTPCH1 ubiquitination. BAECs were pretreated with MG132 (0.5 μmol/L) for 30 min and treated with NG medium (d-glucose, 5 mmol/L) or HG (d-glucose, 30 mmol/L) in the presence of MG132 (0.5 μmol/L) for 6 h. Cell lysates were incubated with ubiquitin interacting motif-agarose for ubiquitin (Ub) AP. Ubiquitinated proteins were analyzed by immunoblotting with anti-GTPCH1 and anti–β-actin antibodies. The blot shown is representative of three independent experiments. IB, immunoblot. *P < 0.05 vs. 5.5 mmol/L.

Next, we investigated the high glucose effects on GTPCH1 ubiquitination. In order to exclude the possible effects of proteasome, the high glucose–induced GTPCH1 ubiquitination was determined in the presence of MG132 (10 μmol/L), a concentration that almost completely inhibited proteasome activity in BAECs (data not shown) and blocked high glucose–induced GTPCH1 reduction (Fig. 6B). Importantly, high glucose markedly increased the levels of ubiquitinated GTPCH1 (approximately threefold) (Fig. 6C).

Antioxidant and ONOO scavenger attenuates high glucose–destabilized GTPCH1.

Because the formation of ONOO requires the simultaneous formation of O2· and NO from NOS, inhibition of either O2· or NO production abolishes the formation of ONOO (1). It was important to further determine whether the high glucose–induced ONOO reduced GTPCH1 stability. As expected, high glucose markedly reduced both GTPCH1 protein levels (Fig. 7A, C, and D) and GTPCH1 activity (Fig. 7B). Importantly, Tempo, a potent antioxidant, abolished the reduction of both GTPCH1 protein (Fig. 7A) and GTPCH1 activity (Fig. 7B). Overexpression of Mn-SOD (Fig. 7C) or Cu-Zn–SOD (not shown) abolished this high glucose–induced GTPCH1 reduction, excluding the potential effects of hydrogen peroxide.

FIG. 7.

ONOO scavenger and antioxidant attenuates high glucose–induced GTPCH1 degradation via endogenous ONOO in endothelial cells. A: UA (100 μmol/L) or Tempo (10 μmol/L) prevented GTPCH1 degradation. BAECs were pretreated with UA or Tempo for 30 min and treated with normal-glucose (NG) medium (d-glucose, 5 mmol/L) or high glucose (HG) (d-glucose, 30 mmol/L) for 3 days. B: GTPCH1 activity. n = 3. C: Adenoviral (AD) overexpression of Mn-SOD prevents GTPCH1 degradation. BAECs were transformed with adenoviral vector GFP or Mn-SOD for 16 h and treated as indicated for 3 days. D: L-NAME (1 mmol/L) prevents GTPCH1 reduction. The blot shown is representative of three independent experiments. NS, not significant. *P < 0.05 vs. NG control; **P < 0.05 30 mmol/L plus UA vs. 30 mmol/L HG; #P < 0.05 30 mmol/L plus Tempo vs. 30 mmol/L HG.

We next determined if ONOO was required for high glucose–induced GTPCH1 reduction and inhibition. L-NG nitro-L-arginine methyl ester (L-NAME) is a nonselective competitive NOS inhibitor. We tested if L-NAME abolished high glucose–induced GTPCH1 degradation. As shown in Fig. 7D, pharmacological inhibition of ONOO formation with L-NAME abolishes the GTPCH1 degradation induced by high glucose. Consistently, uric acid (UA), a potent scavenger of ONOOˉ, abolished the effects of high glucose on GTPCH1 protein (Fig. 7A) and GTPCH1 activity (Fig. 7B). Since UA or Tempo (Fig. 7A) or L-NAME (Fig. 7D) alone did not alter the levels of GTPCH1, these data suggest that high glucose–induced ONOO caused GTPCH1 inhibition and instability.

Diabetes triggers both ONOO and endothelial dysfunction in vivo.

To further determine the effects of ONOO on GTPCH1 ubiquitination and degradation in vivo, C57BL6 mice were made diabetic by STZ injection. Diabetic mice were subsequently treated with vehicle and Tempo (in drinking water, 1 mmol/kg body weight) for 4 weeks. Injection of STZ significantly increased serum glucose whereas Tempo treatment did not alter the blood glucose levels in control mice or STZ-induced diabetic mice (Supplementary Table 1). Body weights in diabetic groups were 15% lower than nondiabetic mice but Tempo treatment did not alter body weight reduction in the STZ-injected group (Supplementary Table 1).

We first determined acetylcholine-triggered endothelium-dependent vasorelaxation. As expected, the maximal endothelial-dependent relaxation in STZ-injected diabetic mice was significantly reduced when compared with nondiabetic control mice (Fig. 8A). In contrast, the endothelium-independent relaxation in response to sodium nitroprusside was not changed in STZ-injected diabetic mice with control nondiabetic mice (data not shown). Although Tempo alone had no effect on the endothelial-dependent maximal relaxation (Fig. 8A), Tempo administration in STZ-injected diabetic mice normalized the maximal relaxation in STZ-injected mice (Fig. 8A).

FIG. 8.

ONOO is involved in GTPCH1 inhibition and GTPCH1 ubiquitination in diabetic mouse aortas in vivo. Control (11 mice) and STZ-induced diabetic mice (12 mice) were fed Tempo as described in research design and methods. A: Tempo treatment normalizes the maximal endothelium-dependent vasorelaxation in diabetic mice. B: Effects of Tempo treatment on total eNOS and the serine 1177 phosphorylation of eNOS in control and STZ-injected diabetic mice. C: Tempo treatment suppresses diabetes-enhanced superoxide production. D: Tempo ablates 3-NT formation in diabetic mouse aortas. E: Tempo abolishes diabetes-induced GTPCH1 inhibition in diabetic mouse aortas. F: Effects of diabetes and Tempo treatment on GTPCH1 ubiquitination in vivo. The total GTPCH1 and ubiquitinated GTPCH1 were assayed in isolated mouse aortas. The blot is a representative of six blots obtained from six independent experiments. n = 6. G: Schematic description for ONOO-induced GTPCH1 inhibition and eNOS uncoupling. Ub, ubiquitin. *P < 0.05 STZ vs. control; #P < 0.05 control + Tempo or L-NAME vs. STZ; †P < 0.05 STZ vs. STZ plus Tempo or L-NAME.

Since eNOS is the main source of NO, it was important to determine if diabetes altered eNOS expression and the serine1177 phosphorylation of eNOS in isolated aortas. The expression of eNOS was markedly increased in STZ-injected aortas when compared with those from non-STZ injected mice (Fig. 8B). Tempo treatment abolished the increase of eNOS in STZ-injected mice (Fig. 8B). In contrast, despite increased eNOS expression in STZ-injected mice, the level of p-eNOS (serine 1177) was reduced in STZ-injected diabetic mice. However, Tempo had no effects on reduced levels of p-eNOS (Fig. 8B) in diabetic mice. Taken together, improved maximal relaxation by Tempo in diabetic mice was unlikely due to improved levels of p-eNOS or eNOS reduction.

It was also interesting to determine if improved maximal relaxation was due to increased production of superoxide anions. As shown in Fig. 8C and Supplementary Fig. 2, STZ significantly increased superoxide anions. Importantly, inhibition of eNOS with L-NAME significantly attenuated STZ-induced superoxide, suggesting that eNOS was the main source of superoxide. Consistently, in STZ-injected diabetic mice, 3-nitrotyrosine (NT)–positive protein, a footprint of ONOO in tissues, was markedly increased (Fig. 8D), which was ablated in Tempo-treated STZ mice (Fig. 8D). Increased 3-NT was also confirmed in diabetic heart tissues. STZ-injected diabetes increased the levels of 3-NT, and Tempo treatment lowered its levels of 3-NT in diabetic hearts (Supplementary Fig. 3A).

ONOO accelerates GTPCH1 ubiquitination and degradation in diabetic aortas and hearts in vivo.

Next, we assayed GTPCH1 activity. As depicted in Fig. 8E and Supplementary Fig. 3B, GTPCH1 activity was inhibited in both diabetic aortas and hearts. Importantly, the administration of Tempo restored GTPCH1 activation in both aortas and hearts (Fig. 8E and Supplementary Fig. 3B).

Finally, we determined whether diabetes accelerated GTPCH1 ubiquitination. To this end, both aortic and heart tissue homogenates of STZ-induced diabetic mice or control mice were used for ubiquitin affinity precipitation (AP), and the coprecipitated proteins with ubiquitin chains were analyzed by Western blot. Compared with the control nondiabetic group, diabetic mouse aortas (Fig. 8F) and hearts (Supplementary Fig. 3CE) exhibited higher levels of ubiquitinated GTPCH1 (approximately fourfold) (Supplementary Fig. 3D). As a result, total levels of GTPCH1 in STZ-induced diabetic aortas and hearts had significantly lower levels of GTPCH1 in the homogenates of diabetic aortas (Fig. 8F) and diabetic hearts (Supplementary Fig. 3E). Taken together, the effects of Tempo in suppressing diabetes-enhanced acceleration of GTPCH1 ubiquitination and degradation and in improving maximal endothelial relaxation in diabetic mice is likely due to its inhibition of GTPCH1 activity in diabetes.

DISCUSSION

In this study, we have for the first time demonstrated that ONOO releases zinc from GTPCH1 and that zinc removal lowers GTPCH1 activity. Further, zinc-deleted GTPCH1 exhibits increased ubiquitination and reduced stability. We found that ONOO generated by high glucose suppresses GTPCH1 activity along with increased ubiquitination and destruction of this enzyme. Finally, GTPCH1 ubiquitination and destruction is markedly increased in parallel with enhanced ONOO in STZ-induced diabetic mice in vivo. Overall, our results suggest that ONOO releases zinc, inhibits GTPCH1 activity, and increases GTPCH1 ubiquitination.

The major finding of this study is that ONOO removes the zinc in GTPCH1, resulting in enzyme inhibition. Several lines of evidence are consistent with the hypothesis that loss of zinc by ONOO oxidation underlies the inactivation of GTPCH1. First, ONOO dose-dependently releases zinc from GTPCH1 (Fig. 1B). Second, the effects of ONOO are mimicked by TPEN, a selective zinc-chelating agent. Finally, GTPCH1 (C141R), a common genetic variant in humans (22) that lacks the essential zinc binding ability due to the substitution of one cysteine, is inactivated. Our results further demonstrate that GTPCH1 zinc is also essential in preventing its ubiquitination and destruction. The ubiquitination-proteasome system is usually considered to degrade unneeded or damaged protein (23,24). Zinc removal resulted in GTPCH1 structure change (10). Modified proteins with the changed conformation may lead to rapid degradation by facilitating their ubiquitination (19,20). In the current study, our results suggest that GTPCH1 zinc removal by ONOO increased its ubiquitination via direct polyubiquitin binding. We further provided the evidence that ONOO dramatically increased GTPCH1 ubiquitination, and the ubiquitinated GTPCH1 shows accelerated destruction. Importantly, C141R mimicked zinc loss of GTPCH1, showed dramatically increased ubiquitination, reduced GTPCH1 protein stability, and accelerated degradation.

Zinc-containing proteins or transcription factors might be important targets for anionic oxidants such as ONOO. In addition to ONOO-mediated GTPCH1 oxidation described above, zinc-containing eNOS is also a target of ONOO (11). Although both eNOS and GTPCH1 contain zinc, zinc in eNOS binds to four cysteines (defined as a zinc-thiolate cluster), whereas zinc in GTPCH1 binds to two cysteines and one histidine (11). In addition, the zinc atom has no catalytic activity in eNOS in which the zinc atom bound two eNOS monomers at the dimer interface, whereas zinc in GTPCH1 is essential in guanine ring opening of GTP. Because of these structural and functional differences, ONOO-induced modifications have different impacts in the enzymes. For example, ONOO-induced zinc release in eNOS does not lead to its ubiquitination or degradation (11), whereas zinc release from GTPCH1 dramatically increases ubiquitination-dependent degradation, as shown in this study. This reaction of ONOO appears to be unique because NO alone did not affect the zinc-thiolate cluster. Conversely, NO but not ONOO markedly increased the detection of S-nitrosylated GTPCH1, whereas ONOO did not have any obvious effect on the S-nitrosylation of GTPCH1 in intact cells. Thus, the mechanism by which ONOO-induced zinc release results in GTPCH1 ubiquitination represents a novel posttranslational modification of zinc-containing proteins.

Increased eNOS-derived O2· and ONOO have been demonstrated in cultured endothelial cells exposed to high glucose, and in diabetic animals and human blood vessels (11,25,26). Studies have indicated that hyperglycemia impairs GTPCH1 activity and reduces GTPCH1 protein levels without any change in mRNA levels (1,27). Our previous report also indicated that hyperglycemia-derived GTPCH1 inactivation is not caused by GFRP (1). Therefore, the posttranslational modification of GTPCH1 is likely the major cause for diabetes-induced GTPCH1 loss. We have previously reported that tyrosine nitration of the proteasome by ONOO produced during hyperglycemia or by angiotensin II increased proteasome activity and accelerated GTPCH1 degradation (1,5). However, the mechanism that caused the preferential degradation of GTPCH1 by proteasome is yet unknown. In this study, we extend our early observations and found that GTPCH1 is a direct target for hyperglycemia-induced ONOO in diabetic mice. Major evidence in support of this notion includes the following. First, we have identified GTPCH1 as a direct target of ONOO in vitro and in cultured endothelial cells. Second, exposure to high glucose increases GTPCH1 ubiquitination, and ONOO scavenger attenuates hyperglycemia-induced GTPCH1 inactivation and degradation. Third, STZ-induced hyperglycemia greatly accelerates GTPCH1 ubiquitination and is associated with the increased ONOO in diabetic mice, which was prevented by administration of Tempo in parallel with the impaired ONOO. Finally, the effects of Tempo in suppressing diabetes-enhanced acceleration of GTPCH1 ubiquitination and degradation might be due to its inhibition of GTPCH1 oxidation in diabetes. Taken together, our results suggested that zinc in GTPCH1 renders it a direct target for ONOO, resulting in zinc loss and the consequent inactivation and ubiquitination. Since the 26S proteasome is also a target of ONOO, and tyrosine nitration of the proteasome by ONOO increased proteasome activity in HUVECs (1), the combinational effects of ONOO on GTPCH1 ubiquitination and proteasome results in an acceleration of GTPCH1 reduction in diabetes. Indeed, we found that the GTPCH1 protein level is lowered in STZ-induced diabetic mice in parallel with increased ubiquitination. Importantly, Tempo, which prevents ONOO production during hyperglycemia, not only prevents GTPCH1 ubiquitination and degradation but also restores GTPCH1 activity.

GTPCH1 degradation or loss of activity results in a rapid decrease in BH4 (1), which is commonly observed in cardiovascular disorders (1,2,5,7,15). BH4 deficiency has been linked to many cardiovascular disorders, including diabetes (14), and hypertension (57,15). For example, diabetic rat (27) and mouse (1) models suggest that reduced BH4 results from decreased GTPCH1 expression and activity without alterations in GTPCH1 mRNA levels (1). Administration of BH4 (28,29) or GTPCH1 gene transfer (15,30) increases BH4 bioavailability and restores endothelial function in diabetes. In deoxycorticosterone acetate–salt hypertensive rats, GTPCH1 activity is decreased during the late stages of hypertension. As a result, eNOS is uncoupled in deoxycorticosterone acetate–salt hypertension because of the reduced essential cofactor BH4. Arterial gene transfer of GTPCH1 restores GTPCH1 activity, restores BH4 levels, and normalizes eNOS function in these animals (31). Accumulating evidence has revealed that during cardiovascular diseases, eNOS becomes “uncoupled,” leading to production of superoxide anions (O2·) rather than NO. The transformation of eNOS from a protective enzyme to a contributor of oxidative stress has been observed in vitro and in vivo, including in patients with cardiovascular risk factors (32,33).

Increasing evidence suggests that loss of BH4 appears to be a common route for endothelial dysfunction in vivo in diabetes, and oral supplementation of BH4 in human type 2 diabetes improves endothelial function (28,34) and insulin sensitivity (35). In type 2 diabetic rats, renal BH4 is considered to play a crucial role in the pathogenesis of diabetic nephropathy (36). Thus, oxidant-induced GTPCH1 ubiquitination and consequent proteasome-mediated degradation represent a common pathway for BH4 loss. Thus, ONOO scavengers, which might be effective in preserving GTPCH1 and BH4 in endothelial cells, might be effective in treating diabetic vascular diseases.

In conclusion, ONOO releases zinc, inhibits GTPCH1 activity, and increases GTPCH1 ubiquitination. Our data support that scavenging of ONOO might help prevent GTPCH1 inactivation and ubiquitination and endothelial dysfunction in diabetes.

ACKNOWLEDGMENTS

This work was supported in part by National Institutes of Health grants (HL-074399, HL-079584, HL-080499, HL-089920, HL-096032, HL-105157, and HL-110448), the Warren Chair in Diabetes Research from the University of Oklahoma Health Sciences Center (all to M.-H.Z.), and a grant (81270355) from the Natural Science Foundation of China. M.-H.Z. is a recipient of the National Established Investigator Award of the American Heart Association.

No potential conflicts of interest relevant to this article were reported.

Y.Z. contributed to the study design, performed experiments, and wrote the manuscript. J.W., H.Z., and P.S. performed some experiments. M.-H.Z. contributed to the study design and interpretation and wrote the manuscript. M.-H.Z. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

  • Received May 8, 2013.
  • Accepted August 14, 2013.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

REFERENCES

| Table of Contents

This Article

  1. Diabetes vol. 62 no. 12 4247-4256
  1. Supplementary Data
  2. All Versions of this Article:
    1. db13-0751v1
    2. 62/12/4247 most recent