β-Cell Failure in Type 2 Diabetes: A Case of Asking Too Much of Too Few?

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FIG. 1.
FIG. 1.

A: Human islets from a nondiabetic subject and a subject with T2DM (upper panel) and from a wild-type (WT) and a human IAPP transgenic (HIP) rat (lower panel) stained for insulin (brown). Deposits of amyloid derived from IAPP are indicated by a white arrowhead. Original magnification: ×40. B: Alignment of IAPP ortholog proteins. Amino acid alignment of IAPP protein sequences identified in Homo sapiens (human, CAA39504), human mutant (S20G), Macaca mulatto (monkey, XP_001098290), Felis catus (cat, NP_001036803), Mus musculus (mouse, NP_034621), and Rattus norvegicus (rat, NP_036718). Dots correspond to conserved residues with human IAPP sequence. Red letters correspond to the amyloidogenic sequence. C: Sections of islets from human IAPP transgenic mice labeled for oligomers (A11) and IAPP (5 nm and 10 nm gold, respectively). IAPP- and oligomer-labeled aggregates were found adjacent to mitochondria (M), and mitochondrial integrity appeared to be compromised (black arrow points to the aggregates penetrating mitochondria). Original magnification: ×120,000. This figure originally appeared in an article by Gurlo et al. (50).

This Article

  1. Diabetes vol. 62 no. 2 327-335