The Neuropathic Diabetic Foot Ulcer Microbiome Is Associated With Clinical Factors
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Comparison of cultivation-based data to genomic 16S rRNA gene data for characterizing DFU bioburden. A: Prediction of bacterial load (log-transformed) by total counts of colony-forming units (CFUs; circles) compared with estimating
bacterial load based on quantitative PCR (qPCR) of the 16S rRNA gene (diamonds). B: Number of different isolates recovered by culturing (circles) vs. the number of species-level OTUs by 16S rRNA gene sequencing
(diamonds). Each point represents a DFU sample and the line through the points represents the median of the data.