Activation of the ACE2/Angiotensin-(1–7)/Mas Receptor Axis Enhances the Reparative Function of Dysfunctional Diabetic Endothelial Progenitors
- Yagna P.R. Jarajapu1,
- Ashay D. Bhatwadekar1,
- Sergio Caballero1,
- Sugata Hazra1,
- Vinayak Shenoy2,
- Reinhold Medina3,
- David Kent4,
- Alan W. Stitt3,
- Catherine Thut5,
- Eva M. Finney5,
- Mohan K. Raizada2⇑ and
- Maria B. Grant1⇑
- 1Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida
- 2Department of Physiology and Functional Genomics, University of Florida, Gainesville, Florida
- 3Center for Vision Science, Queen’s University, Belfast, Ireland
- 4The Vision Clinic, Kilkenny, Ireland
- 5Molecular Profiling and Research Informatics, Merck & Co., Inc., West Point, Pennsylvania
- Corresponding author: Maria B. Grant, , or Mohan K. Raizada, .
We tested the hypothesis that activation of the protective arm of the renin angiotensin system, the angiotensin-converting enzyme 2 (ACE2)/angiotensin-(1-7) [Ang-(1-7)]/Mas receptor axis, corrects the vasoreparative dysfunction typically seen in the CD34+ cells isolated from diabetic individuals. Peripheral blood CD34+ cells from patients with diabetes were compared with those of nondiabetic controls. Ang-(1-7) restored impaired migration and nitric oxide bioavailability/cGMP in response to stromal cell–derived factor and resulted in a decrease in NADPH oxidase activity. The survival and proliferation of CD34+ cells from diabetic individuals were enhanced by Ang-(1-7) in a Mas/phosphatidylinositol 3-kinase (PI3K)/Akt-dependent manner. ACE2 expression was lower, and ACE2 activators xanthenone and diminazine aceturate were less effective in inducing the migration in cells from patients with diabetes compared with controls. Ang-(1-7) overexpression by lentiviral gene modification restored both the in vitro vasoreparative functions of diabetic cells and the in vivo homing efficiency to areas of ischemia. A cohort of patients who remained free of microvascular complications despite having a history of longstanding inadequate glycemic control had higher expression of ACE2/Mas mRNA than patients with diabetes with microvascular complications matched for age, sex, and glycemic control. Thus, ACE2/Ang-(1-7)\Mas pathway activation corrects existing diabetes-induced CD34+ cell dysfunction and also confers protection from development of this dysfunction.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db12-0808/-/DC1.
- Received June 20, 2012.
- Accepted August 22, 2012.
- © 2013 by the American Diabetes Association.
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