Pyruvate carboxylase (PC) ASO improves hepatic insulin sensitivity in HFF rats. Fasting plasma glucose (A) and insulin concentration (B) (n = 9 per group). C: Basal endogenous glucose production (n = 9 per group). Plasma glucose concentration (D) and glucose infusion rate time course (E) during hyperinsulinemic-euglycemic (4 mU/kg per min) clamp, respectively (n = 7–8 per group). Insulin-stimulated peripheral glucose metabolism (F), endogenous glucose production (G), and percentage suppression of endogenous glucose production (H) during clamp (n = 7–8 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with control ASO group. Experiments were done at 4–5 weeks of treatment.