Identification of Sucrose Non-Fermenting–Related Kinase (SNRK) as a Suppressor of Adipocyte Inflammation

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FIG. 4.
FIG. 4.

Effect of SNRK knockdown on adipocyte biology. A: SNRK knockdown by adenovirus-mediated shRNA on mRNA (top panel) and protein (bottom panel) levels. B: Effect of SNRK knockdown on phosphorylation of IKKβ and JNK. C: Effect of SNRK knockdown on lipolysis. D: Effect of SNRK knockdown on expression of TNF-α, IL-6, MCP-1, MCP-2, MCP-3, and chop genes. E: Effect of SNRK knockdown on TNF-α secretion. F: Effect of SNRK knockdown on IL-6 secretion. G: Effect of SNRK knockdown on expression of Glut4, Adipo, VLACAD, MACAD, and Atg12 genes. H: Effect of SNRK knockdown on insulin-stimulated Akt phosphorylation. I: Effect of SNRK knockdown on insulin-stimulated glucose uptake. *P < 0.05. Triplicate samples were used in each experiment. Results shown were representative from three independent experiments. Error bars stand for mean ± SE. S, Ser; Veh, vehicle.

This Article

  1. Diabetes vol. 62 no. 7 2396-2409