Pancreatic intraepithelial neoplasia, endocrine complexes, and cellular replication. Photomicrographs show sections with immunohistochemical
staining for Ki67 (brown) and glucagon (pink) in PanIN lesions (A and C) with hematoxylin counterstain or Ki67 (brown) and insulin (pink) (B and D) with Alcian blue counterstain to highlight mucin. Glucagon-expressing endocrine cells are shown intimately associated with
PanIN lesions to varying degrees. Foci of replication (arrows, Ki67 nuclei) are also apparent. (See Supplementary Fig. 4 for additional examples of foci of increased replication in incretin treated pancreas.) E: Pancreas cell replication is increased in DM-I (Ki67). ***P < 0.0001 DM-I vs. DM and ND. F: Frequency of PanIN1 and 2 (lesions/mm2 ×103 of pancreas) is increased in DM-I. ^P < 0.01 DM-I vs. DM, ***P < 0.0001 DM-I vs. ND.