Resistance to Aerobic Exercise Training Causes Metabolic Dysfunction and Reveals Novel Exercise-Regulated Signaling Networks
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HRT have fewer oxidative muscle fibers and impaired exercise-induced angiogenesis. Plantaris muscles from sedentary (SED)
and exercise-trained (EXT) rats were frozen in N2-cooled isopentane and cut into 6-µm cross-sections. A: Sections were stained with antibodies against laminin (white) and myosin heavy chain I (green) and visualized using fluorescent
secondary antibodies under 100× magnification. Type I fiber content was expressed as % of total muscle fibers counted. B: Capillary density (capillaries/mm2) was calculated in sections stained with an antibody against the endothelial marker CD31 (red). Nuclei were visualized with
DAPI stain (blue). n = 4–5/group. *P < 0.05 for phenotype main effect by two-way ANOVA. P values obtained by Tukey post hoc testing are displayed.