Loss of Krüppel-Like Factor 3 (KLF3/BKLF) Leads to Upregulation of the Insulin-Sensitizing Factor Adipolin (FAM132A/CTRP12/C1qdc2)

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FIG. 1.
FIG. 1.

Klf3−/− mice are lean and resistant to diet-induced obesity. A: Body length of 9-week-old male mice on chow diet (Klf3+/+, n = 8; Klf3−/−, n = 5). Data are means ± SEM. ***P < 0.001. B and C: Male body weight with respect to age. Mice were placed on chow diet (Klf3+/+, n = 48; Klf3+/−, n = 57; and Klf3−/−, n = 11) (B) or high-fat diet (C) from age 3–12 weeks (Klf3+/+, n = 20; Klf3+/−, n = 24; and Klf3−/−, n = 6). DG: Tissue weights as determined by dissection and individual weighing of tissues in chow diet (Klf3+/+, n = 31; Klf3+/−, n = 53; and Klf3−/−, n = 11) (D and F) and high-fat diet (Klf3+/+, n = 25; Klf3+/−, n = 20; and Klf3−/−, n = 6) (E and G). BAT, brown adipose tissue; EPI, epididymal; ING, inguinal subcutaneous; RP, retroperitoneal. Data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, Klf3+/+ vs. Klf3−/−; #P < 0.05, ##P < 0.01, ###P < 0.001, Klf3+/− vs. Klf3−/−. H: Representative photographs of hematoxylin-eosin stained epididymal WAT (×20 magnification) from Klf3+/+ and Klf3−/− male mice fed either chow or high-fat diet. I: Size distribution of epididymal adipocytes. Left panel: Adipocytes from male mice on chow diet. Right panel: Adipocytes from male mice on high-fat diet. Klf3+/+, n = 2–7; Klf3−/−, n = 3–6 (200–300 cells measured per mouse).

This Article

  1. Diabetes vol. 62 no. 8 2728-2737