Adipose Tissue Macrophages Function As Antigen-Presenting Cells and Regulate Adipose Tissue CD4+ T Cells in Mice

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 2.
FIG. 2.

ATMs are the predominant MHC II+ cells in visceral fat. Male C57BL/6 mice were fed ND or HFD for 20 weeks to induce obesity. A: MHC I-A/I-E+ (green) ATMs were identified by epifluorescence microscopy dispersed between adipocytes. Bar = 100 μm. B: Identification of MGL1+ (green) and MHC I-A/I-E+ (red) ATMs in eWAT from ND mice. C and D: MHC I-A/I-E+ (green) ATMs in eWAT from HFD mice. Isolectin (red) stains blood vessels. Bar = 100 μm. E: Concentration of MHC I-A/I-E+ (red) ATMs in MGL1 (green) CLS in eWAT from HFD mice. F: MHC I-A/I-E (green) expression in a FALC in eWAT from HFD mice. Bar = 100 μm. G: H2-Ab1 gene expression in eWAT, iWAT, and spleen from ND and HFD mice. Data are means ± SEM (ND eWAT: n = 5; HFD eWAT: n = 6; iWAT: n = 5; spleen: n = 3 mice per group). H: Ciita gene expression in eWAT and spleen from ND and HFD mice (ND eWAT: n = 5; HFD eWAT: n = 6; spleen: n = 3 mice per group). I: Expression of Ciita transcripts from type I (DC), type III (B cell), and type IV (IFN-γ) promoters in eWAT from ND and HFD mice (ND: n = 5; HFD: n = 6). J: H2-Ab1 expression in FACS-sorted ATMs (F4/80+ CD11b+) and non-ATMs (F4/80 CD11b) from ND and HFD eWAT. Data are means ± SEM (n = 4 per group). K: Surface expression of MHC I-A/I-E on ATMs (F4/80+ CD11b+ SVCs; thick line) and non-ATMs (F4/80 CD11b SVCs; dashed line) isolated from ND and obese HFD mice. Isotype (ISO; shaded) is shown. L: Quantification of ATMs expressing MHC I-A/I-E and non-ATMs in eWAT from ND and HFD mice. Data are means ± SEM (n = 4 per group). M: MHC I-A/I-E expression (MFI, mean fluorescent intensity) on CD11c and CD11c+ ATMs from eWAT of HFD mice as determined by flow cytometry. Data are means ± SEM (n = 4 mice). *P < 0.05, **P < 0.01, ***P < 0.001.

This Article

  1. Diabetes vol. 62 no. 8 2762-2772