Expression of costimulatory molecules on ATMs is regulated by dietary obesity, inflammatory stimuli, and adipocyte signals.
Male C57BL/6 mice were fed ND or HFD for 20 weeks. Cd40, Cd80, and Cd86 expression in eWAT (A), spleen (B), and iWAT (C) from ND and HFD mice. Data are means ± SEM (eWAT: n = 5–6; iWAT: n = 5; spleen: n = 3 mice per group). D: Cd40 expression in FACS-sorted ATMs (F4/80+ CD11b+) and non-ATMs (F4/80− CD11b−) from ND and HFD eWAT. Data are means ± SEM (n = 4 per group). Cd40 (E) and Cd80 (F) expression in cultured SVCs from HFD mice stimulated with IL-4 (20 ng/mL), LPS (100 ng/mL), IFN-γ (5 ng/mL), or LPS (100
ng/mL) plus IFN-γ (5 ng/mL) for 24 h. Data are means ± SEM (n = 3 wells) and are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.