Adipose Tissue Macrophages Function As Antigen-Presenting Cells and Regulate Adipose Tissue CD4+ T Cells in Mice

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FIG. 3.
FIG. 3.

Expression of costimulatory molecules on ATMs is regulated by dietary obesity, inflammatory stimuli, and adipocyte signals. Male C57BL/6 mice were fed ND or HFD for 20 weeks. Cd40, Cd80, and Cd86 expression in eWAT (A), spleen (B), and iWAT (C) from ND and HFD mice. Data are means ± SEM (eWAT: n = 5–6; iWAT: n = 5; spleen: n = 3 mice per group). D: Cd40 expression in FACS-sorted ATMs (F4/80+ CD11b+) and non-ATMs (F4/80 CD11b) from ND and HFD eWAT. Data are means ± SEM (n = 4 per group). Cd40 (E) and Cd80 (F) expression in cultured SVCs from HFD mice stimulated with IL-4 (20 ng/mL), LPS (100 ng/mL), IFN-γ (5 ng/mL), or LPS (100 ng/mL) plus IFN-γ (5 ng/mL) for 24 h. Data are means ± SEM (n = 3 wells) and are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

This Article

  1. Diabetes vol. 62 no. 8 2762-2772