Suppression of Epithelial-to-Mesenchymal Transitioning Enhances Ex Vivo Reprogramming of Human Exocrine Pancreatic Tissue Toward Functional Insulin-Producing β-Like Cells

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FIG. 1.
FIG. 1.

Pancreatic exocrine fractions dedifferentiate toward mesenchymal cells in culture. A: Phase contrast images of exocrine fractions when cultured in tissue culture dishes over a 14-day period. Scale bar = 50 µm. B: QRT-PCR analysis of pancreatic, epithelial, and mesenchymal markers in cultured exocrine fractions from day 2 up to passage 7 after plating in tissue culture dishes. Data are presented as mean ± SEM. Expression levels are relative to glyceraldehyde 3-phosphate dehydrogenase (n = 3). AML, amylase; a-SMA, α-smooth muscle actin; D, day; ECAD, E-cadherin; EPCAM, epithelial cell adhesion molecule; INS, insulin; P, passage; SNAI2, snail homolog 2; VIM, vimentin.

This Article

  1. Diabetes vol. 62 no. 8 2821-2833