Suppression of Epithelial-to-Mesenchymal Transitioning Enhances Ex Vivo Reprogramming of Human Exocrine Pancreatic Tissue Toward Functional Insulin-Producing β-Like Cells

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FIG. 3.
FIG. 3.

Genetic lineage tracing of acinar amylase-positive cells in pancreatic exocrine fractions. A: Schematic representation of the two viral vectors used for tracing amylase-positive cells. B: The dsRed-positive cells were monitored in culture for a 10-day period. The dsRed fluorescence (top row) and brightfield images (bottom row) were analyzed at days 4, 6, 8, and 10. Scale bar = 50 µm. C: Immunocytochemistry was performed on days 3, 7, and 10 on traced amylase-positive cells. The exocrine pancreatic markers amylase (AML) and CK19 were analyzed along with the mesenchymal marker vimentin (VIM) and the proliferation marker ki67. Nuclei were counterstained with DAPI. Scale bar = 20 µm.

This Article

  1. Diabetes vol. 62 no. 8 2821-2833