Suppression of Epithelial-to-Mesenchymal Transitioning Enhances Ex Vivo Reprogramming of Human Exocrine Pancreatic Tissue Toward Functional Insulin-Producing β-Like Cells

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FIG. 4.
FIG. 4.

Ectopic expression of pancreatic TFs reprograms exocrine pancreatic MSCs into glucagon-positive cells. A: Passaged human exocrine pancreatic fractions were plated in tissue culture dishes and subsequently transduced with different combinations of adenoviruses expressing the pancreatic TFs Pdx1 (P), MafA (M), Pax4 (Px), and Ngn3 (N), each with a multiplicity of infection of 100. After 7 days, QRT-PCR was performed and the data were expressed relative to glyceraldehyde 3-phosphate dehydrogenase and presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001. INS, insulin; GLC, glucagon; SST, somatostatin; N/A, nontreated samples. B: Exocrine cells treated with different combinations of adenoviruses expressing P, M, and N in the presence or absence of Px were stained for the expression of glucagon, and cell nuclei were counterstained with DAPI. Scale bar = 20 µm.

This Article

  1. Diabetes vol. 62 no. 8 2821-2833