Inhibition of VEGFR-2 Reverses Type 1 Diabetes in NOD Mice by Abrogating Insulitis and Restoring Islet Function

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FIG. 3.
FIG. 3.

VEGFR inhibition prevents the increases in islet vascularity associated with insulitis. Representative micrographs of purified islets from 4-week-old (A) and new-onset (B) NOD females that were subjected to immunofluorescent staining with anti-CD31 (teal). C: Linear regression analysis shows a positive correlation between CD31+ and Thy1.2+ islet area in islets isolated from prediabetic mice. The micrograph depicts the cumulative data from 67 purified islets analyzed from five mice. D: The islet CD31+ area was increased in purified islets of 18-week-old, prediabetic mice relative to islets from 4-week-old NOD mice. E: A 5-day dosing period of mice with new-onset diabetes with PF-337210 (PF; 20 mg/kg) prevented further increases in islet vascular density. MC, 28 islets analyzed from two mice; PF-337210 (PF), 45 islets analyzed from three mice.

This Article

  1. Diabetes vol. 62 no. 8 2870-2878