Inhibition of VEGFR-2 Reverses Type 1 Diabetes in NOD Mice by Abrogating Insulitis and Restoring Islet Function
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VEGFR inhibition impedes T-cell migration by reducing islet vascularity. A: The proportion of islets infiltrated by BDC2.5 (Thy1.1+) cells 48 h after transfer in PF-337210 (PF)-treated or control mice. The micrograph depicts the cumulative data from 60–100
islets per mouse and 16–18 mice per group. Representative immunofluorescence images of islets purified from MC- (B) or PF-treated (C) mice stained for Thy1.1 (red) and CD31 (teal). D: Enumeration of the number of Thy1.1+ cells per islet in PF-treated mice versus control (MC) mice. E: Vascularity of PF-treated or control mice expressed as the percentage of CD31+ area of the total islet. PF was administered at a dose of 20 mg/kg.