Recovery From Overt Type 1 Diabetes Ensues When Immune Tolerance and β-Cell Formation Are Coupled With Regeneration of Endothelial Cells in the Pancreatic Islets

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FIG. 2.
FIG. 2.

Healthy donor BM cells transferred during immune modulation restore normoglycemia in diabetic mice. A: Schematic representation of the treatment regimen in which diabetic mice received two insulin pellet implants on day 1 of the regimen and Ig-GAD2 and BM transfer as indicated. B: Percent of mice with BGL <300 mg/dL from groups receiving BM transfer alone (BM), Ig-GAD2 alone (Ig-GAD2), or Ig-GAD2 plus male or female BM. The n indicates the number of mice. C and D: SP and PLN cells of the mice receiving BM or Ig-GAD2+BM were harvested on day 70, stimulated with PMA and ionomycin, and analyzed for cytokine production. C: Representative FACS plots (left) and individual mice (right) depicting CD4+CD8Vβ8.1/8.2+ cells producing IFN-γ and IL-10. D: Frequency of IL-17–producing CD4+CD8 cells. E: The RQ of mRNA expression for RORγt (rorc) and T-bet (tbx21) in the pancreatic islets of mice receiving BM or Ig-GAD2+BM. Each bar represents mean ± SEM of four to eight mice. *P < 0.05; **P < 0.01.

This Article

  1. Diabetes vol. 62 no. 8 2879-2889