Donor BM gives rise to pancreatic ECs during suppression of diabetes. A: Confocal microscopy images for insulin (blue), PECAM1 (red), and GFP (green) from mice given Ig-GAD2+NOD.GFP BM (five mice
per time point). The arrows indicate colocalization of PECAM1 and GFP. Scale bars, 10 μm. B–D: PCR analysis of Y chromosome (Y) using genomic DNA extracted from raw pancreatic sections (B), microdissected PECAM1+ (C), or insulin+ cells (D) of mice given Ig-GAD2+male BM (killed on day 60 posttreatment). DNA extracted from unmanipulated male (♂) and female (♀)
mice was included for control purposes. C: Lane 6 represents DNA from an Ig-GAD2+male BM–treated mouse that did not recover from diabetes. D: Lanes 1–9 represent the percentage of male DNA diluted with female DNA. Lanes 10–12 represent DNA from three individual
diabetes-free mice given Ig-GAD2+male BM.