Vascular cell apoptosis analysis in ischemic muscles. Immunofluorescence of apoptotic-positive cells (red) and CD31 (blue)
(top panels) and percentage of apoptotic cells (bottom panel) in the ischemic adductor muscles of nondiabetic (NDM, □) and diabetic (DM, ■) Prkcd+/+ and Prkcd−/− mice. Results are shown as mean ± SD of three sections of six to seven mice per group. White arrows represent colocalization
of CD31 and the apoptotic-positive marker. *P = 0.05 vs. NDM Prkcd+/+, †P < 0.05 vs. DM Prkcd+/+.