PKCδ Impaired Vessel Formation and Angiogenic Factor Expression in Diabetic Ischemic Limbs
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Histological and vascular density analysis. Structural analysis of the ischemic muscles stained with hematoxylin and eosin
(A) and immunofluorescence of endothelial cells (red) and α-smooth muscle actin (green) labeling (B) in the ischemic adductor muscles of nondiabetic (NDM) and diabetic (DM) Prkcd+/+ and Prkcd−/− mice. Quantification of the vascular density (C) and the number of vessels smaller than 50 µm (D). Results are shown as mean ± SD of three sections of six mice per group.