Increased expression of SHP-1 in ischemic adductor muscles of diabetic (DM) and nondiabetic (NDM) mice. Quantitative real-time
PCR of SHP-1 (A), SHP-2, and PTP1B mRNA (B), and protein expression of SHP-1 (C), SHP-2, PTP1B, and their corresponding loading control (actin) (D) in ischemic adductor muscles of NDM and DM Prkcd+/+ and Prkcd−/− mice. Protein expression was detected by immunoblot, and densitometric quantitation was measured. Results are shown as mean
± SD of four to six independent experiments. *P = 0.05 vs. NDM Prkcd+/+, †P < 0.05 vs. DM Prkcd+/+.