Somatostatin Receptor Type 2 Antagonism Improves Glucagon Counterregulation in Biobreeding Diabetic Rats

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FIG. 4.
FIG. 4.

Effects of SSTR2a on glucagon secretion from pancreatic slices of BBDP diabetic and nondiabetic rats. Immunohistochemistry (brown, SSTR2 labeling [i]) and immunofluorescence (red, SSTR2; green, glucagon [ii] or insulin [iii]; merged images) of the localization of SSTR2 to islet α-cells in BBDP diabetic (A) and nondiabetic (B) rat pancreas slices. Insets in ii are enlarged views showing SSTR2 and glucagon colocalization. In Aiii, the few surviving β-cells in diabetic BBDP rats do not contain SSTR2, as is also true in nondiabetic rats (data not shown). Scale bar, 100 μm. Glucagon secretion from pancreatic slices of diabetic BBDP rats (n = 6) (C) and nondiabetic BBDP rats (n = 6) (D). Relative glucagon secretion was calculated as the glucagon secretion from the slices normalized to the total glucagon content of the slices. Secretion rates were calculated using Prism software (GraphPad Software, San Diego, CA). Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. A, arginine; G, glucose.

This Article

  1. Diabetes vol. 62 no. 8 2968-2977