Somatostatin Receptor Type 2 Antagonism Improves Glucagon Counterregulation in Biobreeding Diabetic Rats

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 5.
FIG. 5.

Effects of SSTR2a on glucagon and insulin secretion from normal human pancreatic slices and isolated islets. A: Immunohistochemistry (brown, SSTR2 labeling [i]) and immunofluorescence (red, SSTR2; green, glucagon [ii] or insulin [iii]; merged images) of the localization of SSTR2 to islet α-cells (ii) and β-cells (iii) in normal human pancreas slices. Inset in ii is an enlarged view showing SSTR2 and glucagon colocalization. Scale bar, 100 μm. B: Relative glucagon (top) and insulin (bottom) secretion from pancreatic slices. The filled circles and empty circles represent two sets of perifusion. C: Relative glucagon secretion from isolated pancreatic islets (n = 4). Relative glucagon or insulin secretion was calculated as the glucagon/insulin secretion from the slices/islets normalized to the total glucagon/insulin content of the slices/islets. AUCs were calculated using Prism software (GraphPad Software, San Diego, CA). Data are presented as means ± SEM. **P < 0.01. A, arginine; G, glucose.

This Article

  1. Diabetes vol. 62 no. 8 2968-2977