Interleukin-18 Activates Skeletal Muscle AMPK and Reduces Weight Gain and Insulin Resistance in Mice

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FIG. 2.
FIG. 2.

IL-18R−/− mice exhibit insulin resistance when fed a chow diet and an HFD. A: Plasma insulin (CON-chow: n = 7; CON-HFD: n = 8; IL-18R−/− -chow: n = 8; IL-18R−/− -HFD: n = 6). B: Insulin tolerance with area under the curve (AUC) inserted (CON-chow: n = 12; CON-HFD: n = 12; IL-18R−/− -chow: n = 14; IL-18R−/− -HFD: n = 12) For the actual data, see Supplementary Fig. 1. Total and phosphorylated Ser473 during chow diet (C; n = 4–6) and HFD (D) (n = 8–9) in muscle and total and phosphorylated Ser473 during chow diet (E; n = 5–6) and HFD (F; n = 7–9) in liver. Total Akt was performed separately and was not stripped. The signaling data were obtained in littermate controls. Samples from chow diet and HFD were run on separate gels. Results are presented as mean ± SEM . *P <0.05; **P <0.01, ***P <0.001 vs. chow (A) or vs. basal (CF). #P <0.05; ##P <0.01 vs. control (A, C). §Interaction between genotype and diet P = 0.06 (E). CON, control; Ins, insulin; pAkt, phosphorylated Akt.

This Article

  1. Diabetes vol. 62 no. 9 3064-3074