PRLR Regulates Hepatic Insulin Sensitivity in Mice via STAT5

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FIG. 3.
FIG. 3.

Knocking down PRLR using Ad-shPRLR decreases insulin (Ins) sensitivity in vivo. Male C57BL/6J mice were infected with Ad-shPRLR (+ Ad-shPRLR) or scrambled vector (− Ad-shPRLR) via tail-vein injection, followed by examination of PRLR expression in the liver at day 7 in A, measurement of blood glucose and serum insulin levels at day 14 in B and C, calculating HOMA-IR index in D, performance of GTTs and ITTs at day 3 or 4 in E, and examination of insulin signaling in liver before (− Ins) and after (+ Ins) 2 units/kg insulin stimulation for 3 min at day 7 in F. The mean ± SEM values shown are representative of at least two independent in vivo experiments, with the number of mice included in each group in each experiments indicated (n = 5–6). Statistical significance was calculated using the two-tailed Student t test for the effects of the Ad-shPRLR vs. the control group (*P < 0.05). A: Prlr mRNA and PRLR protein (top, Western blot; bottom, quantitative measurement of PRLR protein relative to actin). B: Blood glucose levels. C: Serum insulin levels. D: HOMA-IR index. E: GTT and ITT. F: p-IR (tyr1150/1151), p-Akt (ser473), and PRLR protein (left, Western blot; right, quantitative measurements of p-IR and p-Akt protein relative to their total protein). t, total.

This Article

  1. Diabetes vol. 62 no. 9 3103-3113