Lower absolute cell numbers in the draining lymph nodes of TGF-β–expressing grafts. Total numbers of cells retrieved from
the graft-draining RLNs (draining RLNs [dRLNs]) of TGF-β–expressing grafts were lower than those draining WT grafts. The nondraining
RLNs (control RLN [cRLNs]) had lower total numbers of cells than the graft-draining lymph nodes (A). The composition of the cell populations in the lymph nodes was similar with regard to CD8+ T (B) and CD4+ T cells (C). Within the CD4+ T-cell population, percentages of Foxp3+ Treg cells also were similar between groups (D). Each symbol (●, ○, ■, and □) represents the result from one mouse, and differences between groups were assessed using the
Wilcoxon signed rank test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.