Metabolic characteristics of weight-cycling mice. Male C57Bl/6 mice were placed on a 10% LFD or a 60% HFD for three 9-week
intervals. A: Study design. B–F: Body weight and composition were determined. Body weight timeline for the duration of the study (B). Body weight after 27 weeks (Wk) of diet (C). Nuclear magnetic resonance was used to determine lean mass (D) and fat mass (E) after 27 weeks of diet. F: Epididymal AT weight after 27 weeks of diet. Data are presented as mean ± SEM; n = 15–17/group. Groups not connected by the same letter are significantly different, P < 0.05 (e.g., the value for the group labeled “a” is significantly different from the group labeled “b”). White squares,
LF/LF/LF (lean control group); gray squares, LF/HF/HF (weight-gain group); black triangles, HF/LF/HF (weight-cycling group).