Weight cycling does not modulate ATM content. SVF cells were isolated from the epididymal AT and analyzed by flow cytometry.
A and B: LF/LF/LF (lean control) group. C and D: LF/HF/HF (weight gain) group. E and F: HF/LF/HF (weight cycling) group. Cells were gated for the macrophage population based upon forward and side scatter (Supplementary Fig. 1) and analyzed for macrophage markers. Representative flow cytometry histogram of F4/80 (A, C, and E). Percentages on histograms represent percent of macrophage-gated cells that are F4/80 positive. Flow cytometry dot plot
of F4/80+ cells analyzed for CD11b and CD11c (B, D, and F). G: Quantification of ATM flow cytometry (shown as percent of fluorophore-positive cells relative to all live SVF cells) for
all groups. Data are presented as mean ± SEM; n = 6–12/group. Groups not connected by the same letter within each cell population are significantly different; P < 0.05. HF/LF/HF, weight-cycling group; LF/HF/HF, weight-gain group; LF/LF/LF, lean control group.