Weight cycling does not modulate ATM phenotype. RNA was isolated from the epididymal AT and gene expression was analyzed by
real-time RT-PCR. A: Emr1. B: Cd68. C: Itgam. D: Itgax. E: Tnf. F: Ccr2. G: Arg1. H: Clec10a. I: M2-to-M1 macrophage ratio calculated by dividing the relative expression of either Arg1 or Clec10a (M2 markers) by the relative expression of Itgax (M1 marker). Data are presented as mean ± SEM; n = 8–14/group. Groups not connected by the same letter are significantly different; P < 0.05. HF/LF/HF, weight-cycling group; LF/HF/HF, weight-gain group; LF/LF/LF, lean control group.