Weight cycling increases AT T-cell populations. SVF cells were isolated from the epididymal AT and analyzed by flow cytometry.
LF/LF/LF (lean control) group (A and B), LF/HF/HF (weight gain) group (C and D), HF/LF/HF (weight cycling) group (E and F). Cells were gated for the lymphocyte population based upon forward and side scatter (Supplementary Fig. 1) and analyzed for T-cell markers. Representative flow cytometry histogram of TCR-β (A, C, and E). Percentages on histograms represent percent of lymphocyte-gated cells that are TCR-β–positive. Flow cytometry dot plot
of TCR-β+ cells analyzed for CD4 and CD8 (B, D, and F). G: Quantification of AT T-cell flow cytometry (shown as percent of fluorophore-positive cells relative to all live SVF cells)
from all groups. Data are presented as mean ± SEM; n = 10–12/group. Groups not connected by the same letter within each cell population are significantly different; P < 0.05. HF/LF/HF, weight-cycling group; LF/HF/HF, weight-gain group; LF/LF/LF, lean control group.