MicroRNA-24/MODY Gene Regulatory Pathway Mediates Pancreatic β-Cell Dysfunction

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FIG. 5.
FIG. 5.

Analysis of cellular phenotypes by silencing of Hnf1a and Neurod1. Silencing efficiencies of Hnf1a and Neurod1 were measured at mRNA (A) and protein (B) levels using qRT-PCR and Western blotting, respectively (**P < 0.01 vs. siNC). Results demonstrated that the siRNAs were effective. C: DNA synthesis was assessed by BrdU labeling. Hnf1a and Neurod1 were both able to inhibit DNA synthesis, reflecting the capacity to reduce proliferation. This inhibition was enhanced by knocking down both proteins (**P < 0.01 vs. siNC). D: GSIS assay was performed 48 h post-transfection of indicated siRNAs, and the released insulin was normalized to corresponding total insulin content. Basal and stimuli-induced insulin release were both repressed upon downregulation of Hnf1a or Neurod1 (*P < 0.05 or **P < 0.01 vs. siNC).

This Article

  1. Diabetes vol. 62 no. 9 3194-3206