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Oxidative stress induced miR-24 expression. MIN6 cells were precultured in DMEM with 5.5 mmol/L glucose for 24 h, and then
cells were incubated with the indicated concentration of H2O2 for an additional 24 h or with glucose for an additional 48 h. Relative expression of miR-24 induced by H2O2 (A) or glucose (B) was analyzed by TaqMan qRT-PCR relative to corresponding controls. U6 detected by a TaqMan probe was used as an internal control. Values are the mean ± SEM of three individual experiments (*P < 0.05; **P < 0.01). C and D: The protein levels of Hnf1a and Neurod1 were observed by Western blotting. β-Tubulin was used as an internal control.