Vagal Hyperactivity Due to Ventromedial Hypothalamic Lesions Increases Adiponectin Production and Release
- Yoko Suzuki1,2,
- Hiroyuki Shimizu1,3,
- Noriko Ishizuka1,
- Naoto Kubota4,
- Tetsuya Kubota4,
- Akira Senoo1,
- Haruaki Kageyama1,
- Toshimasa Osaka2,
- Satoshi Hirako5,6,
- Hyoun-Ju Kim6,
- Akiyo Matsumoto6,
- Seiji Shioda5,
- Masatomo Mori7,
- Takashi Kadowaki4 and
- Shuji Inoue1⇑
- 1Faculty of Health Science, Kiryu University, Midori, Gunma, Japan
- 2Health Promotion and Exercise Program, National Institute of Health and Nutrition, Shinjuku-ku, Tokyo, Japan
- 3International University of Health and Welfare Hospital, Otawara, Tochigi, Japan
- 4Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan
- 5Department of Anatomy, Showa University School of Medicine, Shinagawa-ku, Tokyo, Japan
- 6Department of Clinical Dietetics and Human Nutrition, Josai University, Sakado, Saitama, Japan
- 7Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
- Corresponding author: Shuji Inoue, .
In obese humans and animals, adiponectin production and release in adipose tissue are downregulated by feedback inhibition, resulting in decreased serum adiponectin. We investigated adiponectin production and release in ventromedial hypothalamic (VMH)-lesioned animals. VMH-lesioned mice showed significant increases in food intake and body weight gain, with hyperinsulinemia and hyperleptinemia at 1 and 4 weeks after VMH-lesioning. Serum adiponectin was elevated in VMH-lesioned mice at 1 and 4 weeks, despite adipocyte hypertrophy in subcutaneous and visceral adipose tissues and increased body fat. Adiponectin production and mRNA were also increased in both adipose tissues in VMH-lesioned mice at 1 week. These results were replicated in VMH-lesioned rats at 1 week. Daily atropine administration for 5 days or subdiaphragmatic vagotomy completely reversed the body weight gain and eliminated the increased adiponectin production and release in these rats, with reversal to a normal serum adiponectin level. Parasympathetic nerve activation by carbachol infusion for 5 days in rats increased serum adiponectin, with increased adiponectin production in visceral and subcutaneous adipose tissues without changes of body weight. These results demonstrate that activation of the parasympathetic nerve by VMH lesions stimulates production of adiponectin in visceral and subcutaneous adipose tissues and adiponectin release, resulting in elevated serum adiponectin.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-0636/-/DC1.
- Received April 22, 2013.
- Accepted January 26, 2014.
- © 2014 by the American Diabetes Association.
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