Activating HSP72 in Rodent Skeletal Muscle Increases Mitochondrial Number and Oxidative Capacity and Decreases Insulin Resistance
- Darren C. Henstridge1,
- Clinton R. Bruce1,2,
- Brian G. Drew3,
- Kálmán Tory4,
- Attila Kolonics4,
- Emma Estevez1,
- Jason Chung1,
- Nadine Watson1,
- Timothy Gardner1,
- Robert S. Lee-Young1,
- Timothy Connor5,
- Matthew J. Watt2,
- Kevin Carpenter6,
- Mark Hargreaves7,
- Sean L. McGee5,
- Andrea L. Hevener3 and
- Mark A. Febbraio1,4⇑
- 1Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
- 2Department of Physiology, Monash University, Clayton, Victoria, Australia
- 3Division of Endocrinology, Diabetes and Hypertension, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA
- 4N-Gene Research Laboratories, Inc., Budapest, Hungary
- 5Metabolic Research Unit, School of Medicine, Deakin University, Geelong, Victoria, Australia
- 6Department of Biochemical Genetics, Children’s Hospital at Westmead and Disciplines of Genetic Medicine and Paediatrics and Child Health, University of Sydney, New South Wales, Australia
- 7Department of Physiology, University of Melbourne, Parkville, Victoria, Australia
- Corresponding author: Mark A. Febbraio, .
D.C.H. and C.R.B. contributed equally to this study.
Induction of heat shock protein (HSP)72 protects against obesity-induced insulin resistance, but the underlying mechanisms are unknown. Here, we show that HSP72 plays a pivotal role in increasing skeletal muscle mitochondrial number and oxidative metabolism. Mice overexpressing HSP72 in skeletal muscle (HSP72Tg) and control wild-type (WT) mice were fed either a chow or high-fat diet (HFD). Despite a similar energy intake when HSP72Tg mice were compared with WT mice, the HFD increased body weight, intramuscular lipid accumulation (triacylglycerol and diacylglycerol but not ceramide), and severe glucose intolerance in WT mice alone. Whole-body VO2, fatty acid oxidation, and endurance running capacity were markedly increased in HSP72Tg mice. Moreover, HSP72Tg mice exhibited an increase in mitochondrial number. In addition, the HSP72 coinducer BGP-15, currently in human clinical trials for type 2 diabetes, also increased mitochondrial number and insulin sensitivity in a rat model of type 2 diabetes. Together, these data identify a novel role for activation of HSP72 in skeletal muscle. Thus, the increased oxidative metabolism associated with activation of HSP72 has potential clinical implications not only for type 2 diabetes but also for other disorders where mitochondrial function is compromised.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-0967/-/DC1.
- Received June 19, 2013.
- Accepted January 9, 2014.
- © 2014 by the American Diabetes Association.
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