Adipose-Specific Knockout of Seipin/Bscl2 Results in Progressive Lipodystrophy
- Lu Liu1,
- Qingqing Jiang1,
- Xuhong Wang2,
- Yuxi Zhang3,
- Ruby C.Y. Lin3,
- Sin Man Lam4,
- Guanghou Shui4,
- Linkang Zhou5,
- Peng Li5,
- Yuhui Wang1,
- Xin Cui1,
- Mingming Gao1,
- Ling Zhang1,
- Ying Lv6,
- Guoheng Xu6,
- George Liu1⇑,
- Dong Zhao2⇑ and
- Hongyuan Yang3⇑
- 1Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Peking University Health Science Center, Beijing, China
- 2Department of Endocrinology, Lu He Teaching Hospital of the Capital Medical University, Beijing, China
- 3School of Biotechnology and Biomolecular Sciences, the University of New South Wales, Sydney, NSW, Australia
- 4State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
- 5MOE Key Laboratory of Bioinformatics and Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
- 6Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China
- Corresponding authors: George Liu, ; Dong Zhao, ; and Hongyuan Yang, .
L.L., Q.J., and X.W. contributed equally to this work.
Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) is the most severe form of human lipodystrophy, characterized by an almost complete loss of adipose tissue and severe insulin resistance. BSCL2 is caused by loss-of-function mutations in the BSCL2/SEIPIN gene, which is upregulated during adipogenesis and abundantly expressed in the adipose tissue. The physiological function of SEIPIN in mature adipocytes, however, remains to be elucidated. Here, we generated adipose-specific Seipin knockout (ASKO) mice, which exhibit adipocyte hypertrophy with enlarged lipid droplets, reduced lipolysis, adipose tissue inflammation, progressive loss of white and brown adipose tissue, insulin resistance, and hepatic steatosis. Lipidomic and microarray analyses revealed accumulation/imbalance of lipid species, including ceramides, in ASKO adipose tissue as well as increased endoplasmic reticulum stress. Interestingly, the ASKO mice almost completely phenocopy the fat-specific peroxisome proliferator–activated receptor-γ (Pparγ) knockout (FKO-γ) mice. Rosiglitazone treatment significantly improved a number of metabolic parameters of the ASKO mice, including insulin sensitivity. Our results therefore demonstrate a critical role of SEIPIN in maintaining lipid homeostasis and function of adipocytes and reveal an intimate relationship between SEIPIN and PPAR-γ.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-0729/-/DC1.
- Received May 7, 2013.
- Accepted March 9, 2014.
- © 2014 by the American Diabetes Association.
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