Insulin Inhibits Cardiac Contractility by Inducing a Gi-Biased β2-Adrenergic Signaling in Hearts
- Qin Fu1,2,3,
- Bing Xu1,
- Yongming Liu1,4,
- Dippal Parikh1,
- Jing Li5,
- Ying Li5,
- Yuan Zhang6,7,
- Christian Riehle6,7,
- Yi Zhu6,
- Tenley Rawlings6,
- Qian Shi1,3,
- Richard B. Clark8,
- Xiongwen Chen5,
- E. Dale Abel6,7 and
- Yang K. Xiang1,3⇑
- 1Department of Pharmacology, University of California, Davis, Davis, CA
- 2Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
- 3Department of Molecular and Integrative Physiology, University of Illinois at Urbana, Urbana, IL
- 4Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
- 5Department of Physiology and Cardiovascular Research Center, Temple University Medical Center, Philadelphia, PA
- 6Division of Endocrinology, Metabolism, and Diabetes, Program in Molecular Medicine, University of Utah, Salt Lake City, UT
- 7Fraternal Order of Eagles Diabetes Research Center and Division of Endocrinology and Metabolism, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA
- 8Department of Integrative Biology and Pharmacology, University of Texas Houston Medical Center, Houston, TX
- Corresponding author: Yang K. Xiang, .
Insulin and adrenergic stimulation are two divergent regulatory systems that may interact under certain pathophysiological circumstances. Here, we characterized a complex consisting of insulin receptor (IR) and β2-adrenergic receptor (β2AR) in the heart. The IR/β2AR complex undergoes dynamic dissociation under diverse conditions such as Langendorff perfusions of hearts with insulin or after euglycemic-hyperinsulinemic clamps in vivo. Activation of IR with insulin induces protein kinase A (PKA) and G-protein receptor kinase 2 (GRK2) phosphorylation of the β2AR, which promotes β2AR coupling to the inhibitory G-protein, Gi. The insulin-induced phosphorylation of β2AR is dependent on IRS1 and IRS2. After insulin pretreatment, the activated β2AR-Gi signaling effectively attenuates cAMP/PKA activity after β-adrenergic stimulation in cardiomyocytes and consequently inhibits PKA phosphorylation of phospholamban and contractile responses in myocytes in vitro and in Langendorff perfused hearts. These data indicate that increased IR signaling, as occurs in hyperinsulinemic states, may directly impair βAR-regulated cardiac contractility. This β2AR-dependent IR and βAR signaling cross-talk offers a molecular basis for the broad interaction between these signaling cascades in the heart and other tissues or organs that may contribute to the pathophysiology of metabolic and cardiovascular dysfunction in insulin-resistant states.
Insulin and adrenergic stimulation represent two divergent regulatory systems that interact with overlapping signaling pathways in adipocytes, liver, and skeletal and cardiac muscle. Hyperinsulinemia is a uniform characteristic of obesity and type 2 diabetes (1), which increases insulin receptor (IR) signaling in the myocardium (2). It was recently demonstrated that hyperactivation of insulin signaling in the myocardium contributes to adverse left ventricular (LV) remodeling in pressure overload cardiac hypertrophy (induced by transverse aortic constriction) (3). Heart failure, which is associated with elevated sympathetic adrenergic activity, is characterized by generalized insulin resistance, hyperinsulinemia (4), and impaired insulin-mediated glucose uptake in the myocardium (2). Diabetes and obesity increase the risk of heart failure and induce cardiac dysfunction, which has been termed diabetic cardiomyopathy (5,6). Given that dysfunction of these regulatory systems commonly occurs in cardiovascular diseases (7–9), it is likely that molecular cross-talk between insulin and adrenergic receptor regulatory systems exists within the cardiovascular system.
Stimulation of β-adrenergic receptors (βARs), which are prototypical members of the G-protein–coupled receptor superfamily, is best known for its regulation of contractile function in the heart. Ligand binding to βARs induces cAMP-dependent protein kinase A (PKA) activation (10) leading to phosphorylation of various substrates including phospholamban (PLB) (10–12) to increase myocyte contractility, stroke volume, and cardiac output (13). Among the cardiac βARs, β1AR is the major subtype that couples to the stimulatory G protein, Gs, to stimulate contractile function, whereas β2AR is able to couple to both Gs and Gi but with minimal effect on contractile function (10–12). Conversely, activation of IRs, which are receptor tyrosine kinases, promotes phosphorylation of IR substrates (IRS-1 and -2) leading to Akt activation, which promotes glucose uptake, glucose metabolism, and insulin-mediated cardiac and skeletal muscle growth (14). Stimulation of βARs also increases glucose uptake in cardiac and skeletal muscle cells (15,16). Insulin and adrenergic stimulation share common downstream signaling components including Gi (17), arrestin (18), and G-protein receptor kinase (GRK)2 (16,19,20). Stimulation with either insulin or adrenergic receptors antagonizes the ability of the other to activate glucose transport (8) and to modulate myocyte survival (21).
An earlier study suggested that insulin augmented adrenergic stimulation of contractility in isolated papillary muscles (22). However, in an ischemia-reperfusion study, insulin inhibited β-adrenergic responses in the heart(s) (23). Also, phosphatidylinositol 3-kinase, a downstream kinase in the insulin signaling pathway, inhibits β-adrenergic–induced contractile responses in isolated cardiomyocytes (24). Earlier studies revealed that insulin induced β2AR phosphorylation and internalization in HEK293 cells and adipocytes (25–27); however, a comprehensive understanding of the molecular mechanisms underlying insulin’s effects on β2AR signaling in the heart remains to be achieved.
In this study, we characterized signaling cross-talk in which IRs and β2ARs form a novel complex in the heart. This complex directly exerts a β2AR-dependent impact on intracellular transduction of βAR signaling pathways that regulate cardiac contractility in cardiomyocytes and the myocardium. Stimulation of IR promotes IRS-dependent and GRK2-mediated phosphorylation of β2AR in isolated cardiomyocytes and in ex vivo Langendorff perfused hearts or after euglycemic-hyperinsulinemic clamps in vivo. Stimulation of the IR also promotes dissociation of the β2AR-IR complex and promotes β2AR internalization. Internalization of β2AR selectively promotes Gi coupling to attenuate cAMP/PKA signaling (28), which inhibits contractile response in isolated neonatal and adult cardiomyocytes and in Langendorff perfused hearts. Our results not only underscore the critical role of signaling cross-talk and integration between IR and βARs for contractile regulation in the myocardium but also provide a potential general mechanism to understand cross-talk between IR and βAR regulatory systems in other metabolic disorders and cardiac diseases.
Research Design and Methods
Langendorff Perfusion Heart Preparation
Animal experiments were performed following the National Institutes of Health Guide for the Care and Use of Laboratory Animals. All procedures were approved by the Institutional Animal Care and Use Committees at the University of California, Davis; the University of Utah; Temple University; and the Carver College of Medicine of the University of Iowa. The isolated heart perfusion technique was described previously (29). Hearts were excised from mice under anesthesia (120 mg/kg body wt i.p. sodium pentobarbital) and were rapidly placed on a Langendorff apparatus. Hearts were perfused at a constant pressure of 80 mmHg with a solution containing 113.8 mmol/L NaCl, 22 mmol/L NaHCO3, 4.7 mmol/L KCl, 1.2 mmol/L KH2PO4, 1.1 mmol/L MgSO4, 11 mmol/L glucose, 2 mmol/L CaCl2, and 2 mmol/L Na-pyruvate and aerated with 95% oxygen and 5% carbon dioxide, pH 7.35–7.4, to which was added isoproterenol (ISO) at concentrations ranging from 10-14 to 10-6 (mol/L). A water-filled balloon was inserted into the left ventricle and adjusted to achieve a LV end diastolic pressure of 10 mmHg. The balloon was connected to a Millar pressure system (Millar Instruments, Houston, TX), and the pressure was measured with a pressure catheter (SPR-671; Millar Instruments) connected to an ADInstruments PowerLab 16/30 with LabChart Pro-6.0 (ADInstruments, Boston, MA). The heart rate was maintained at 480 bpm by pacing the right ventricle with a Grass SD9 Stimulator. Once a stable effect of the previous dose (∼5 min) was obtained, the next dose was applied. LV pressure, LV end diastolic pressure, and the maximum rate of positive and negative change (dP/dt) in LV pressure were recorded. Data were analyzed offline with LabChart Pro-6.0.
Euglycemic-hyperinsulinemic clamps were performed in nonsedated mice as previously described with minor changes (30). In summary, the mouse jugular vein was catheterized under tribromoethanol anesthesia (250 mg/kg body wt by single intraperitoneal injection). Mice were allowed to recover for 5 days with one heparin flush on day 3 before the clamp procedure. All mice were fasted overnight before the clamp procedure day to synchronize the metabolic state. On the day of the procedure, mice were single housed in a standard housing cage with a tether arm attached to the catheter. A dual infusion pump (Harvard Apparatus, Boston, MA) was used to infuse insulin at a constant flow rate (10 mU/kg/min). A glucose solution was infused at a variable rate to maintain plasma glucose at a target value of 75–110 mg/dL and held at that level for 60 min. A comparable rate of saline infusion was used as control. Glucose was monitored using tail vein blood at 5-min intervals with a glucometer (Glucometer Elite; Bayer, Tarrytown, NY).
Isolated Working Heart Perfusions and Intraperitoneal Injection
Mouse hearts were isolated from anesthetized mice and perfused in the working mode in Krebs-Henseleit buffer supplemented with 5 mmol/L glucose and 0.4 mmol/L palmitate in the presence or absence of 1 nmol/L insulin as previously described by our group (31). Cardiac-restricted IRS1-KO and IRS2-KO mice were injected with isoproterenol (ISO) (2 mg/kg) for 10 min. The hearts were harvested for Western blot.
Neonatal cardiomyocytes were isolated from 1- to 2-day-old wild-type, β1AR-KO, and β2AR-KO and cardiac-restricted IRS1-KO and IRS2-KO mouse pups. Adult mouse cardiomyocytes were isolated from wild-type and mutant (mut) mice as indicated and cultured as described previously (32). Adult rat cardiomyocytes were provided by Dr. Donald Bers (University of California, Davis). In a subset of experiments, H9c2 cardiomyoblasts were cultured in DMEM plus 10% FBS for experiments.
Adenovirus Infection and Plasmid Transfection
Neonatal and adult cardiomyocytes were infected with adenoviruses (100 multiplicity of infection) as previously described to express the PKA activity biosensor (A-kinase activity reporter 3 [AKAR3] ) or the cAMP biosensor (indicator of cAMP using epac 3 [ICUE3] ) or C terminal of inhibitory G protein [Gi-ct], GFP-βARKct, Flag-β2AR, Flag-GRKmut β2AR, or Flag-PKAmut β2AR (28) as indicated for 24 h. Small interfering RNA oligos targeting the mouse IR (IDT, Coralville, IA) were transfected into wild-type neonatal cardiomyocytes, and experiments were conducted after 48 h expression. GRK2, IRS1, and IRS2 mouse small hairpin RNA (shRNA) plasmids (Sigma-Aldrich, St. Louis, MO) were used to create recombinant lentiviruses. Neonatal cardiomyocytes were infected with GRK2 shRNA, IRS1 shRNA, or IRS2 shRNA lentivirus for 24 h and cultured for an additional 48 h.
Fluorescent Resonance Energy Transfer Measurement
Myocytes expressing PKA or cAMP biosensors were rinsed and maintained in PBS for fluorescent resonance energy transfer (FRET) recordings (32). Cells were imaged on a Zeiss Axiovert 200M microscope with a 40×/1.3NA oil-immersion objective lens and a cooled charge-coupled device (CCD) camera. Dual emission ratio imaging was acquired with a 420DF20 excitation filter, a 450DRLP diachronic mirror, and two emission filters (475DF40 for cyan and 535DF25 for yellow). The acquisition was set with 200-ms exposure in both channels and 20-s elapses. Images in both channels were subjected to background subtraction, and ratios of yellow-to-cyan color were calculated at different time points.
Adult Myocyte-Shortening Assay
Cells were stimulated with ISO at indicated concentrations after treatment with or without insulin (100 nmol/L) for 30 min. Adult myocytes were placed in a dish with HEPES buffer (34) and electrically stimulated at 30 V/cm at 0.5 Hz at room temperature. Cell length was recorded with a charge coupled device camera. Cell contraction shortening was analyzed by IonOptix software (IonOptix, Boston, MA) and normalized as the increase over the basal levels after being fitted to a sigmoidal curve. The maximal shortening was normalized to the baseline value.
Western Blot Analysis
Whole-cell and heart tissue lysates were prepared in lysis buffer (50 mmol/L Tris, pH 7.4; 2.5 mmol/L EDTA; 150 mmol/L NaCl, 25 mmol/L sodium pyrophosphate, and 1% (v/v) NonidetP40, 1% Na-deoxycholate, 0.1% SDS, and protease inhibitor cocktail tablets (Thermo Scientific, Chicago, IL) after washing twice with ice-cold PBS. The lysates without boiling were resolved by SDS-PAGE. Proteins were transferred to a Nitrocellulose membrane (Millipore, Billerica, MA), and incubated with the primary antibody followed by IRDye 680CW goat-anti mouse or with IRDye 800CW goat-anti rabbit secondary antibodies. Specific proteins were detected by an Odyssey scanner (LI-COR, Lincoln, NE). The primary antibodies used for Western blotting were as follows: total and phosphorylated PLB at Ser16 and Thr17 (Bradilla, Leeds, U.K.), IR (SCBT, Santa Cruz, CA), total and phosphorylated β2AR at Ser261/262 (28) and Ser355/356 (SCBT), total and phosphorylated pAkt Ser473 (Cell Signaling, Danvers, MA), γ-tubulin, GFP, and GRK2 (SCBT).
Heart tissues were used to detect endogenous protein interactions. Heart tissues were lysed with a FastPrep-24 homogenizer for 20 s in immunoprecipitation assay buffer (50 mmol/L Tris-HCl [pH 7.5], 150 mmol/L NaCl, 1%NP-40, 0.25% deoxycholate, 9.4 mg/50 mL sodium orthovanadate, and 1% sodium dodecyl sulfate). Lysates were cleared by centrifugation (40,000 rpm for 30 min at 4°C) and subjected to immunoprecipitation with Protein A beads (Repligen, Waltham, MA). The immunoprecipitates were resolved via SDS-PAGE and blotted with antibodies against IR (1:500) or β2AR (1:500). Primary antibodies were visualized with IRDye 680CW goat anti-mouse or with IRDye 800CW goat anti-rabbit secondary antibodies using an Odyssey scanner (LI-COR).
One or two-way ANOVA followed by post hoc Turkey test or Student t test were performed using Prism (GraphPad Software, San Diego, CA). P < 0.05 was considered statistically significant.
Insulin Impairs β-Adrenergic Stimulation of Contractility in Mouse Hearts
We hypothesized that insulin could impair adrenergic signal transduction in the myocardium and impair adrenergic-induced contractile responses. In Langendorff perfused mouse hearts, insulin alone (1 nmol/L) did not significantly affect cardiac contractility in mouse hearts as evidenced by LV developed pressure (LVDP) (57.5 ± 13.6 mmHg without insulin vs. 53.3 ± 4.6 mmHg with insulin), maximal +dP/dt (rate of rise of ventricular pressure) (1,944.8 ± 508.9 mmHg/s without insulin vs. 1,766.0 ± 202.9 mmHg/s with insulin), and minimum –dP/dt (rate of fall of ventricular pressure) (−1,520.5 ± 318.2 mmHg/s without insulin vs. −1,344.7 ± 115.8 mmHg/s with insulin). β-Adrenergic stimulation with ISO induced a dose-dependent increase in cardiac contractility (Fig. 1A–C) with half-maximal effective concentration (EC50) of ∼0.01 nmol/L and maximal response at concentrations >1 nmol/L. ISO increased peak LVDP from 57.5 ± 13.6 to 132.7 ± 5.6 mmHg (130.7%), maximal +dP/dt from 1,944.8 ± 508.9 to 5,868.3 ± 981.5 mmHg/s (201.7%), and minimum −dP/dt from −1,520.5 ± 318.2 to −4,351.8 ± 358.3 mmHg/s (186.2%). In contrast, pretreatment with 1 nmol/L of insulin significantly attenuated the ISO-induced contractile responses, changing the EC50 by 5- to 10-fold (∼0.1 nmol/L). In comparison with hearts stimulated with ISO alone, the maximal responses of LVDP (from 53.3 ± 4.6 to 78.9 ± 10.0 mmHg; 48.0%), +dP/dt (from 1,766.0 ± 202.9 to 2,584.5 ± 156.2 mmHg/s; 46.4%), and –dP/dt (from −1,344.7 ± 115.8 to −2,077.7 ± 164.8 mmHg/s; 54.5%) were all substantially decreased (Fig. 1A–C). Consistent with contractility data, adrenergic stimulation, but not insulin stimulation, significantly induced PKA phosphorylation of Ser16 and Ca2+/calmodulin-dependent protein kinase (CaMK)II phosphorylation of Thr17 of PLB, a critical regulator of calcium cycling that mediates cardiac contractility. In comparison, neither stimulation altered the expression of SERCA and the ratio between PLB and SERCA (Supplementary Fig. 1). However, pretreatment with insulin significantly reduced PKA and (CaMK)II phosphorylation of PLB induced by ISO (Fig. 1D). These data suggest that insulin blunts β-adrenergic responsiveness of cardiomyocytes, which may contribute to impaired cardiac function in hyperinsulinemic states.
Activation of IR by Insulin Impairs β-Adrenergic Signaling in Cardiomyocytes in a β2AR-Dependent Manner
We then used FRET-based biosensors AKAR3 (33) for PKA activities to directly determine the impact of insulin-adrenergic signaling cross-talk on cAMP and PKA activities in neonatal cardiomyocytes. Stimulation of βAR with ISO induced a dose-dependent increase in the PKA AKAR3-FRET ratio (EC50 0.43 nmol/L) (Fig. 2A and Supplementary Fig. 2). Insulin dose-dependently impaired ISO-induced increase(s) in the PKA AKAR3-FRET ratio by right shifting the ISO-induced dose response curves (EC50 3.78 nmol/L at 10 nmol/L and EC50 38.2 nmol/L at 100 nmol/L of insulin treatment) (Fig. 2A). Pretreatment with increasing doses of insulin almost completely inhibited the increase(s) in PKA AKAR3-FRET ratio induced at 1 nmol/L of ISO (Fig. 2A). ISO (100 nmol/L) induced a robust increase in sarcomere shortening in adult cardiomyocytes (Fig. 2B). While insulin alone minimally affected the baseline shortening, pretreatment with insulin significantly attenuated the ISO-induced myocyte shortening (Fig. 2B). In comparison, insulin did not affect the forskolin-induced cAMP FRET response and myocyte contractile shortening response (Supplementary Fig. 3). To further assess which βAR subtype signaling was modulated by insulin, we applied the β1AR selective agonist dobutamine or the β2AR selective agonist clenbuterol. Dobutamine robustly increased the PKA AKAR3-FRET ratio, which was significantly attenuated after pretreatment with insulin (Fig. 2C). In comparison, clenbuterol induced a modest increase in PKA AKAR3-FRET ratio, which was also reduced by pretreatment with insulin (Fig. 2C). In agreement, dobutamine, but not clenbuterol, significantly increased myocyte contractile shortening, which is consistent with the predominant role of β1AR in promoting cardiac contractility in hearts (10–12). In contrast, β2AR-induced cAMP and PKA activities are compartmentalized along the plasma membrane and play a minimal role in inducing phosphorylation of PLB and the contractile response (10–12). Thus, whereas pretreatment with insulin significantly attenuated the dobutamine-induced contractile response (Fig. 2D), insulin had little impact on contractility when cells were exposed to clenbuterol. Consistent with the contractile shortening data, dobutamine but not clenbuterol induced a strong phosphorylation of Ser16 and Thr17 of PLB, which was significantly attenuated by insulin pretreatment (Fig. 2E–H).
We further used myocytes from mice lacking individual βAR genes to determine which βAR subtype is involved in insulin-induced signaling cross-talk. In wild-type neonatal myocytes, insulin pretreatment reduced the ISO-induced changes in cAMP ICUE3 and PKA AKAR3-FRET ratios (Fig. 3A and B). In myocytes lacking β2ARs (β2AR-KO), we examined the insulin effect on β1AR, the major β1AR subtype that is responsible for cardiac contractile response to catecholamines in the heart. Interestingly, insulin did not influence ISO-induced and β1AR-mediated increases in cAMP and PKA FRET ratio (Fig. 3A and B), suggesting that expression and activation of β2ARs is necessary for the insulin effect. As controls, in myocytes lacking β1ARs (β1AR-KO), activation of β2AR induced small increases in cAMP and PKA FRET ratios, which were sensitive to insulin pretreatment (Fig. 3A and B). These data indicate that despite the minor expression of β2AR in the heart, the cross-talk between IR and β2AR is sufficient to attenuate the cAMP signal induced by activation of both β1AR and β2AR in wild-type cells.
We then validated the cross-talk between IR and βAR subtype signaling cascades in contractile shortening in adult cardiomyocytes. ISO failed to increase contractile shortening or phosphorylation of PLB in β1AR-KO myocytes (Fig. 3C and D). In contrast, in β2AR-KO myocytes, ISO induced a robust response in contractile shortening, which was not affected by insulin pretreatment (Fig. 3E). Consistently, ISO also promoted a strong phosphorylation of Ser16 and Thr17 of PLB, which was not affected by insulin pretreatment (Fig. 3F).
β2AR Is Necessary for the Inhibitory Effect of Insulin on Cardiac Contractility in Mouse Hearts
We further validated the necessary role of β2AR in cross-talk between IR and βAR signaling cascades in animal hearts. In Langendorff perfused β2AR-KO mouse hearts, β-adrenergic stimulation with ISO induced a strong increase in cardiac contractility, including increased peak LVDP, maximal +dP/dt, and minimum −dP/dt (Fig. 4A–C). Unlike the case of wild-type hearts, pretreatment with 1 nmol/L of insulin did not significantly attenuate the ISO-induced contractile responses, with no reduction in the maximal responses of LVDP, maximal +dP/dt, or minimal −dP/dt (Fig. 4A–C). Consistent with contractility data, adrenergic stimulation, but not insulin stimulation, significantly induced PKA phosphorylation of Ser16 and (CaMK)II phosphorylation of Thr17 of PLB (Fig. 4D). Pretreatment with insulin did not significantly reduce ISO-induced PKA and (CaMK)II phosphorylation of PLB (Fig. 4D). These data confirm the essential role of β2AR in mediating the cross-talk between IR and βARs that impairs cardiac contractility in animal hearts.
IR and β2AR Exist in a Complex, and Insulin Induces PKA- and GRK-Mediated Phosphorylation of β2AR in Murine Hearts
We then sought to determine the molecular mechanism for the IR/β2AR cross-talk in animal hearts. Isolated mouse hearts were perfused in the Langendorff mode with or without insulin (1 nmol/L) for 5 min. We observed significant increase(s) in phosphorylation of β2AR at Ser261/262 (PKA sites) and Ser355/356 (GRK sites) relative to saline-perfused controls (Fig. 5A). Meanwhile, in the hearts of mice subjected to euglycemic-hyperinsulinemic clamps for 60 min, we also observed significant increase(s) in phosphorylation of β2AR at both PKA and GRK sites in hearts obtained from hyperinsulinemic animals (Fig. 5B). Activation of IR signaling was supported by increased phosphorylation of Akt in hearts exposed to insulin ex vivo and in vivo (Fig. 5A and B). In comparison, the protein levels of Gi were not changed by insulin perfusion (Supplementary Fig. 4). Moreover, we found that IRs and β2ARs form membrane complexes that may facilitate signaling cross-talk. Coimmunoprecipitation with anti-IR antibody showed that both receptors form complexes in mouse hearts, but the association was significantly reduced in hearts after exposure to insulin for 10 min ex vivo (Fig. 5C) or in mouse hearts after euglycemic-hyperinsulinemic clamps for 60 min in vivo (Fig. 5D).
Insulin Induces IRS-Dependent PKA and GRK Phosphorylation of β2AR for Biased Activation of Gi in Cardiomyocytes
Activation of IR induces downstream signaling via recruitment of the adaptor signaling proteins, insulin receptor substrates (IRSs). Deletion of either IRS1 orIRS2 in mouse hearts (35) abolished insulin-induced phosphorylation of β2AR and the inhibitory effect of insulin (100 nmol/L) on the ISO-induced cAMP FRET responses (Fig. 6A and B). Inhibition of IRS autophosphorylation with PQ401 also abolished the inhibitory effect of insulin (100 nmol/L) on the ISO-induced cAMP FRET responses (Fig. 6C). Accordingly, silencing of the IR with an IR-specific small interfering RNA abolished the insulin effects on the βAR-induced cAMP signal (Fig. 6D). These data suggest that cross-talk between IR and βARs are dependent on the interaction between the IR and IRS proteins. A recent study indicates that insulin can promote formation of IRS-GRK2 complexes in animal hearts (16). While different GRKs are implicated in agonist-induced phosphorylation of β2ARs in a cell type–specific manner (28,36), we have previously shown that GRK2 is necessary for βAR agonist–induced GRK phosphorylation of β2ARs at Ser355/356 in cardiomyocytes (28). Inhibition of GRK2 also abolished the insulin-induced phosphorylation of β2ARs at GRK sites Ser355/356 in H9C2 cardiomyoblasts (Supplementary Fig. 5A) and the insulin-mediated impairment of β-adrenergic stimulation of cAMP signaling in neonatal cardiomyocytes (Fig. 7A). Meanwhile, the PKA inhibitor H89 abolished the insulin-induced phosphorylation of β2ARs at PKA sites Ser261/262 in H9C2 cardiomyoblasts (Supplementary Fig. 5B) and partially rescued the insulin-mediated impairment of ISO-induced cAMP FRET response in neonatal cardiomyocytes (Fig. 7B). As a control, H89 minimally affected the maximal cAMP signaling induced by ISO alone (Fig. 7B). We further examined the role of β2AR phosphorylation in IR-β2AR cross-talk by introducing either wild-type or mutant β2ARs into β2AR-KO neonatal cardiomyocytes. Insulin impaired cAMP generation induced by ISO in cells expressing the wild-type β2AR, but the effect of insulin was absent in the cells expressing either PKAmut β2AR that lacks the PKA phosphorylation sites or GRKmut β2AR that lacks the GRK phosphorylation sites (Fig. 7C). In agreement, inhibition of GRK2 by overexpressing βARKct, a dominant negative inhibitor of GRK2, abolished the inhibitory effect of insulin on the ISO-induced PKA FRET response in neonatal myocytes and fractional shortening in adult rat cardiomyocytes (Fig. 7D and E).
The phosphorylation of the β2AR by GRK2 and PKA promotes receptor internalization and also switches the receptor coupling from Gs to Gi proteins in cardiomyocytes (28,37). In myocytes treated with insulin (100 nmol/L for 30 min), inhibition of Gi with pertussis toxin (PTX) or the specific Gi inhibitor Gi-CT (38) rescued the ISO-induced cAMP FRET response (Fig. 8A). Moreover, inhibition of Gi with PTX abolished the inhibitory effect of insulin on ISO-induced phosphorylation of PLB and contractile shortening in adult cardiomyocytes (Fig. 8B). Activation of IR has been reported to be linked to activation of phosphodiesterase 3 (PDE3) in oocytes and neurons (39,40). We therefore tested whether PDE3 plays any role in the inhibitory effect of insulin on βAR signaling in myocytes. Inhibition of PDE3 with cilostamide partially rescued the ISO-induced cAMP FRET response (Supplementary Fig. 6). These data suggest that insulin induces a β2AR-Gi coupling to inhibit cAMP production and myocyte contractility under adrenergic stimulation in part via activation of PDE3.
In both diabetes and heart failure, circulating insulin levels are chronically elevated, leading to persistent stimulation of IRs in these clinical conditions. Despite the resistance to Akt-mediated glucose metabolism in adipocytes and skeletal muscle cells, the heart retains its insulin sensitivity in terms of insulin’s ability to activate IR signaling cascades in type 2 diabetes (2,41). The increase in insulin signaling in myocardium promotes translocation of CD36, which exacerbates fatty acid uptake and lipotoxicity (42). Moreover, hyperactive insulin signaling also accelerates adverse LV remodeling in pressure overload hypertrophy (3). Here, we show that insulin can directly impair adrenergic signaling pathways for contractile function via an IR-β2AR signaling complex in animal hearts. This study offers a potential novel mechanism for cardiac dysfunction associated with hyperinsulinemia in diabetic cardiomyopathy and heart failure.
In animal hearts, this IR and β2AR signaling complex channels a direct and negative impact of insulin on β-adrenergic signaling pathways that stimulate cardiac contractile function. These data are consistent with previous reports showing that insulin inhibits β-adrenergic action in hearts after ischemia/reperfusion (23). Insulin stimulation promotes cross-talk with β2AR pathways via IRS-dependent and GRK2-mediated phosphorylation of the adrenergic receptor, which selectively activates a Gi-biased β2AR-signaling cascade to inhibit cAMP/PKA activities under β-adrenergic stimulation. Consequently, this IR-β2AR cross-talk leads to impaired β-adrenergic–induced contractile function in cardiomyocytes and perfused mouse hearts (Fig. 8C).
Previous studies show that insulin induces phosphorylation of the β2AR at classic PKA phosphorylation sites for β2AR internalization and subsequent downregulation in human embryonic kidney (HEK)293 cells and adipocytes (25–27). Here, we identified additional phosphorylation of the β2AR at the classic GRK sites after insulin stimulation, which are also dependent on IRS expression (Fig. 6) (28). Notably, the GRK-mediated phosphorylation of β2AR in cardiomyocytes is different from those in HEK293 cells (36). In cardiomyocytes, GRK2 is necessary for agonist-induced phosphorylation of Ser355/356 (28), which we now show is promoted by activation of the IR. The phosphorylation at GRK sites is likely due to recruitment of GRK2 to the β2AR via increased association between GRK2 and IRS proteins after insulin stimulation (16). The signaling pathways involved in PKA phosphorylation of β2AR remain to be elucidated. A prior study reported that IR signaling intermediates downstream of phosphatidylinositol 3-kinase may play a role in inhibiting cAMP-mediated inotropic effects under adrenergic stimulation (24), which could be linked to phosphorylation at the PKA sites of β2AR.
After insulin stimulation, the phosphorylated β2AR dissociates from the complex and promotes receptor/Gi coupling. Thus, insulin signaling mimics a biased β2AR agonist that selectively activates a Gi-biased signaling pathway. This insulin-induced β2AR/Gi coupling is sufficient to attenuate adrenergic-induced cAMP activities in hearts. As a result, insulin blunts adrenergic-induced PKA phosphorylation of PLB, a critical protein involved in myocyte calcium cycling, and impairs β-adrenergic–induced contractility in both isolated myocytes and animal hearts. A prior study revealed that IGF-1 can promote β1AR internalization in HEK293 cells and inhibit β1AR signaling in canine adult cardiomyocytes (43). Conversely, overexpression of IGF-1 in the myocardium prevents streptozotocin-induced cardiac contractile dysfunction and restored β-adrenergic responsiveness in isolated myocytes (44). Therefore, it remains to be determined whether IGF-1 signaling modulates β1AR signaling in cardiomyocytes via mechanisms that are distinct from those of insulin.
Accumulating evidence also indicates that adrenergic signaling may modulate insulin signal transduction pathways that regulate glucose uptake in adipocytes and skeletal and cardiac muscle cells (8,16,18,20). Reduced β2AR expression in aged animals contributes to glucose intolerance, and overexpression of the β2AR can rescue this phenotype (45). Evidence also suggests that Akt could be a key mechanism underlying the impact of β-adrenergic stimulation on insulin-induced glucose uptake (46,47), although precise molecular signaling mechanisms remain to be elucidated. This newly identified IR-β2AR complex herein reported may provide a molecular basis to understand and reconcile recent studies showing that βAR activation impairs insulin-induced Akt activation, GLUT4 translocation, and glucose uptake in cardiomyocytes, adipocytes, and skeletal muscle cells (16,19,48,49).
The novel observations made in this report are of translational significance. Diabetes and insulin resistance are associated with altered cardiac structure and function independently of coronary artery disease and ischemia (50). Moreover, impaired inotropic reserve to dobutamine was observed in humans with type 2 diabetes in the absence of coronary artery disease (51). Given that type 2 diabetes in humans is associated with hyperinsulinemia and activation of myocardial insulin signaling (2), our findings provide a plausible mechanism for impaired myocardial inotropic reserve in individuals with type 2 diabetes. Diabetes also increases the risk of heart failure (52), and heart failure is an insulin-resistant state (2). If the present findings that hyperinsulinemia may inhibit β1AR signaling via Gi-biased β2AR signaling hold true in humans, then the possibility is raised that hyperinsulinemic subjects with type 2 diabetes and heart failure might have increased sensitivity to cardio-depressive effects of nonselective or β1 blockade, which currently represent the standard of care for managing patients with heart failure. Furthermore, the current study raises the interesting question of whether specific modulation of β2-adrenergic receptor signaling could influence the pathophysiology of diabetic cardiomyopathy or play a role in managing heart failure in diabetic or obese patients with hyperinsulinemia. More broadly, the IR-βAR signaling cross-talk herein described offers a new platform with which to explore the broad implications of myocardial insulin signaling in heart failure, which is an insulin-resistant and hyperinsulinemic state.
Acknowledgments. The authors thank Dr. Donald Bers (University of California, Davis) for reagents.
Funding. This study was supported by National Institutes of Health grants RO1 HL082846 to Y.K.X. and DK092065 to E.D.A., who is an established investigator of the American Heart Association (AHA); AHA established investigator grant 12EIA8410007 to Y.K.X. and AHA 0730347N to X.C.; National Natural Science Foundation of China grant 81102438 to Q.F.; and a postdoctoral fellowship from the German Research Foundation to C.R.
Duality of Interest. No potential conflicts of interest relevant to this article were reported.
Author Contributions. Q.F. wrote and edited the manuscript and researched data. B.X., Y.Liu, D.P., J.L., Y.Li, Y.Zha., Y.Zhu, T.R., Q.S., and X.C. researched data. C.R. and R.B.C. provided essential reagents and materials. E.D.A. and Y.K.X. wrote and edited the manuscript and researched data. Y.K.X. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-1763/-/DC1.
- Received November 19, 2013.
- Accepted March 19, 2014.
- © 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.