Control of Insulin Secretion by Cholinergic Signaling in the Human Pancreatic Islet
- Judith Molina1,
- Rayner Rodriguez-Diaz1,2,3,
- Alberto Fachado2,
- M. Caroline Jacques-Silva2,
- Per-Olof Berggren2,3,4⇑ and
- Alejandro Caicedo1,2,5,6⇑
- 1Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL
- 2Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL
- 3The Rolf Luft Research Center for Diabetes & Endocrinology, Karolinska Institutet, Stockholm, Sweden
- 4Division of Integrative Biosciences and Biotechnology, WCU Program, University of Science and Technology, Pohang, Korea
- 5Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, FL
- 6Program in Neuroscience, Miller School of Medicine, University of Miami, Miami, FL
- Corresponding authors: Per-Olof Berggren, , and Alejandro Caicedo, .
Acetylcholine regulates hormone secretion from the pancreatic islet and is thus crucial for glucose homeostasis. Little is known, however, about acetylcholine (cholinergic) signaling in the human islet. We recently reported that in the human islet, acetylcholine is primarily a paracrine signal released from α-cells rather than primarily a neural signal as in rodent islets. In this study, we demonstrate that the effects acetylcholine produces in the human islet are different and more complex than expected from studies conducted on cell lines and rodent islets. We found that endogenous acetylcholine not only stimulates the insulin-secreting β-cell via the muscarinic acetylcholine receptors M3 and M5, but also the somatostatin-secreting δ-cell via M1 receptors. Because somatostatin is a strong inhibitor of insulin secretion, we hypothesized that cholinergic input to the δ-cell indirectly regulates β-cell function. Indeed, when all muscarinic signaling was blocked, somatostatin secretion decreased and insulin secretion unexpectedly increased, suggesting a reduced inhibitory input to β-cells. Endogenous cholinergic signaling therefore provides direct stimulatory and indirect inhibitory input to β-cells to regulate insulin secretion from the human islet.
This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-1371/-/DC1.
- Received September 5, 2013.
- Accepted March 15, 2014.
- © 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.