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Pathophysiology

GLP-1R Agonists Modulate Enteric Immune Responses Through the Intestinal Intraepithelial Lymphocyte GLP-1R

  1. Bernardo Yusta1,
  2. Laurie L. Baggio1,
  3. Jacqueline Koehler1,
  4. Dianne Holland1,
  5. Xiemin Cao1,
  6. Lee J. Pinnell2,
  7. Kathene C. Johnson-Henry2,
  8. William Yeung2,
  9. Michael G. Surette3,
  10. K.W. Annie Bang1,
  11. Philip M. Sherman2 and
  12. Daniel J. Drucker1⇑
  1. 1Department of Medicine, Mount Sinai Hospital, Lunenfeld-Tanenbaum Research Institute, Toronto, ON, Canada
  2. 2Cell Biology Program, Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada
  3. 3Department of Medicine, Department of Biochemistry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada
  1. Corresponding author: Daniel J. Drucker, drucker{at}lunenfeld.ca.
Diabetes 2015 Jul; 64(7): 2537-2549. https://doi.org/10.2337/db14-1577
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Abstract

Obesity and diabetes are characterized by increased inflammation reflecting disordered control of innate immunity. We reveal a local intestinal intraepithelial lymphocyte (IEL)-GLP-1 receptor (GLP-1R) signaling network that controls mucosal immune responses. Glp1r expression was enriched in intestinal IEL preparations and copurified with markers of Tαβ and Tγδ IELs, the two main subsets of intestinal IELs. Exendin-4 increased cAMP accumulation in purified IELs and reduced the production of cytokines from activated IELs but not from splenocytes ex vivo. These actions were mimicked by forskolin, absent in IELs from Glp1r−/− mice, and attenuated by the GLP-1R agonist exendin (9-39) consistent with a GLP-1R–dependent mechanism of action. Furthermore, Glp1r−/− mice exhibited dysregulated intestinal gene expression, an abnormal representation of microbial species in feces, and enhanced sensitivity to intestinal injury following administration of dextran sodium sulfate. Bone marrow transplantation using wild-type C57BL/6 donors normalized expression of multiple genes regulating immune function and epithelial integrity in Glp1r−/− recipient mice, whereas acute exendin-4 administration robustly induced the expression of genes encoding cytokines and chemokines in normal and injured intestine. Taken together, these findings define a local enteroendocrine-IEL axis linking energy availability, host microbial responses, and mucosal integrity to the control of innate immunity.

Introduction

The enteroendocrine system comprises a complex network of specialized endocrine cells situated within the epithelial lining of the stomach and small and large intestine. Gut endocrine cells synthesize and secrete dozens of peptide hormones that function as local neurotransmitters, propagating signals through engagement of the enteric nervous system. Enteroendocrine-derived hormones also act as classical hormones controlling metabolism through regulation of energy intake, digestion, absorption, and the disposal and storage of digested nutrients (1). The majority of gut hormones are secreted at low basal levels in the fasting state, and feeding produces a rapid rise in circulating levels, enabling activation of gut hormone action in response to energy intake. Plasma levels of some gut hormones, including the glucagon-like peptides, also increase rapidly in the presence of intestinal injury or mucosal inflammation (2).

Proglucagon-producing L cells are among the most intensively studied enteroendocrine cells predominantly localized to the distal small bowel and colon (3). Proglucagon liberates multiple proglucagon-derived peptides with unique biological activities, including glicentin, oxyntomodulin, and GLP-1 and GLP-2 (4). Although the actions of glicentin and oxyntomodulin remain incompletely understood, both GLP-1 and GLP-2 exert well-defined actions through distinct G-protein–coupled receptors. GLP-1 controls gut motility and food intake and stimulates insulin while simultaneously inhibiting glucagon secretion (4,5). In contrast, GLP-2 acts more locally within the gut to increase nutrient absorption and maintain the integrity of the gut epithelium (6).

Considerable evidence supports the importance of the GLP-1 system as an integral component of the incretin axis. Indeed, transient blockade of GLP-1 receptor (GLP-1R) signaling reduces insulin, increases glucagon, and impairs glucose tolerance in multiple species (4). Similarly, genetic interruption of the murine Glp1r impairs glucose control, establishing basal GLP-1R signaling as essential for regulation of β-cell function and glucose homeostasis. Nevertheless, the low levels of circulating active GLP-1 together with the distal location of L cells have led some to question whether the major role of GLP-1 is to function as a circulating incretin hormone (7).

More recent studies have expanded concepts of L-cell biology to encompass control of GLP-1 secretion by cytokines and bacterial products. Interleukin (IL)-6 enhances secretion of GLP-1, actions mediated by direct interaction of IL-6 with gut L cells (8). GLP-1 secretion is also induced by bacterial endotoxin, enabling lipopolysaccharide (LPS)-mediated glucoregulation through the GLP-1R (9,10). Furthermore, plasma GLP-1 levels are significantly increased in hospitalized critically ill patients (10), supporting a correlation between systemic inflammation and L-cell secretion. Nevertheless, the importance of GLP-1 in the setting of localized or systemic inflammation remains uncertain. We describe a new axis linking GLP-1R signaling to the control of immune responses in intestinal intraepithelial lymphocytes (IELs), a specialized gut immune cell population that modulates innate immunity and controls gut barrier function, epithelial turnover, and the host response to enteric pathogens (11). These findings extend our understanding of enteroendocrine-immune interactions beyond traditional glucoregulatory concepts of GLP-1 action, with implications for use of GLP-1R agonists in the treatment of metabolic disorders characterized by enhanced activity of the innate immune system.

Research Design and Methods

Mice

Male or female C57BL/6 wild-type (WT) mice were from The Jackson Laboratory (Bar Harbor, ME) and acclimated to the animal facility for a minimum of 1 week before analysis. Whole-body Glp1r−/− mice on the C57BL/6 genetic background (12) and Glp1r+/+ control mice were generated by crossing Glp1r+/− mice; 9–18-week-old animals from the same litter or family were studied and housed under specific pathogen-free conditions in microisolator cages and maintained on a 12-h light/dark cycle with free access to standard rodent diet and water unless otherwise noted. All experiments were carried out in accordance with protocols and guidelines approved by the Animal Care Committee at the Toronto Centre for Phenogenomics.

Isolation of Mouse Intestinal IELs, Splenocytes, and Thymocytes

Murine intestinal IELs were obtained following established protocols (13). After flushing luminal contents and removing Peyer’s patches, intestines were opened longitudinally and cut in 5–10-mm pieces. Tissue fragments were incubated twice in 10 mmol/L calcium- and magnesium-free Hanks’ balanced salt solution containing 1 mmol/L dithiothreitol and 5% FBS at 37°C followed by vortexing. Supernatants were combined, filtered through a 40-μm strainer, and pelleted (crude IEL preparations). IELs were further purified by centrifugation in a discontinuous 44/67% Percoll gradient. IELs were collected at the interface (Percoll-purified IEL preparations). At this stage, cells were routinely 50–75% lymphocytes, with epithelial elements accounting for the remainder. Mouse splenocytes and thymocytes were isolated by mechanical dissociation of the spleen and thymus, respectively, followed by red blood cell lysis.

cAMP Analysis

Sorted IELs, splenocytes, or thymocytes were allowed to recover for 1 h and then challenged for 15 min at 37°C with exendin-4 (Ex-4) (CHI Scientific, Maynard, MA), prostaglandin E2 (5 μmol/L; Sigma-Aldrich Canada), or the adenylyl cyclase activator forskolin (Fk) in Hanks’ balanced salt solution buffer containing 0.1% BSA and 300 μmol/L 3-isobutyl-1-methylxanthine. cAMP concentration was measured using a LANCE cAMP Detection Kit (PerkinElmer, Waltham, MA) and an EnVision Multilabel Plate Reader (PerkinElmer).

Activation Assays in IELs and Splenocytes

Freshly isolated splenocytes and sorted untouched IELs were stimulated in vitro with plate-bound anti-CD3 (clone 145-2C11) and soluble anti-CD28 (clone 37.51) monoclonal antibodies (mAbs) in culture medium (RPMI + 10% FBS + 50 μmol/L β-mercaptoethanol) in the presence or absence of Ex-4, exendin (9-39) (Bachem, Torrance, CA), or Fk. After incubation for 5 h at 37°C, cells were harvested for quantitative RNA and cAMP analyses.

Dextran Sodium Sulfate–Induced Colitis

Glp1r+/+ or Glp1r−/− female mice were maintained on drinking water containing 3% (weight for volume) dextran sodium sulfate (DSS) (molecular weight 40,000–50,000; USB, Cleveland, OH) or regular drinking water ad libitum for 7 days. Water intake and body weights were determined daily. Animals were euthanized on day 8, and for each mouse, a disease activity index score was calculated (14). Colon damage scores were determined as described (15). In acute studies, C57BL/6 male mice were maintained on drinking water containing 3% (weight for volume) DSS ad libitum for 4 days. On the fourth day, mice were switched to regular drinking water and given two subcutaneous injections of 10 nmol/kg Ex-4 or PBS separated by 12 h. Mice were killed the following morning for analysis of the small bowel and colon.

Histology and Immunohistochemistry

Intestinal tissue segments were fixed in 10% neutral buffered formalin and paraffin embedded. Digital image acquisition and morphometry were performed on 5-μm histological sections stained with hematoxylin-eosin as described (16). To quantify IELs, sections were stained with a rabbit polyclonal anti-CD3 antibody (Dako Canada, Burlington, ON, Canada) and counterstained with hematoxylin. A minimum of 20 villi/section from three to four sections per tissue sample were scored.

Generation of Bone Marrow Chimeras

Bone marrow chimeras were generated by lethally irradiating Glp1r+/+ or Glp1r−/− female mice (1,100 cGy split into two equal doses 4 h apart) followed by reconstitution with 5 × 106 bone marrow cells from donor C57BL/6 male mice as described (17). Mice were euthanized 12–14 weeks after bone marrow transplantation. Chimerism was assessed by quantitative PCR (qPCR) analysis for the sex-determinant Y chromosome (Sry) using spleen DNA from recipient mice. The efficiency of reconstitution of WT Glp1r expression within the intestinal compartment of recipient mice was assessed by qPCR.

RNA Isolation and Analysis of mRNA Expression

Total RNA was extracted using guanidinium isothiocyanate (18). cDNA synthesis and assessment of full-length Glp1r mRNA expression were performed as described (16,19). Real-time qPCR was performed on an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA) with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (Applied Biosystems). Quantification of transcript levels was performed by the 2-ΔCt method using 18S rRNA, cyclophilin, Tbp, or β-actin for normalization.

Gut Microbiota Analysis

Fecal DNA extraction and 16S rRNA gene amplification were carried out as previously described (20). Microbial population similarities within and between groups of Glp1r+/+ and Glp1r−/− mice were determined using metagenomic analysis as described (21). A cluster analysis tree was generated using UPGMA (unweighted pair group method with arithmetic mean) cluster analysis of Bray-Curtis dissimilarity coefficients.

Statistical Analysis

Except where indicated, data are presented as mean ± SD or SE. Statistical significance was determined by unpaired two-tailed Student t test or one- or two-way ANOVA with Bonferroni post hoc analysis using GraphPad Prism version 5.02 software (San Diego, CA). P < 0.05 was considered statistically significant.

Results

The Glp1r Is Expressed in Murine Intestinal IELs

Recent studies highlighted limitations of commercially available antisera for detection of immunoreactive GLP-1R expression (16,22). To localize cellular sites of intestinal Glp1r expression beyond the enteric nervous system (23), we fractionated the murine small intestine by sequential collagenase digestion followed by analysis of Glp1r expression and simultaneous assessment of cell lineage–specific RNA transcripts. Quantitatively, Glp1r expression segregated with markers of the villus epithelial compartment (Supplementary Fig. 1A, fraction 1) rather than the crypt compartment, mesenchyme, or smooth muscle layer (Supplementary Fig. 1A, fraction 2). Unexpectedly, a marked increase in relative Glp1r expression was observed in RNA obtained from intestinal cell preparations enriched in IELs (Fig. 1A and B). Consistent with IEL Glp1r expression, we detected abundant expression of IEL markers such as integrin α E (Itgae) and the T-cell surface glycoprotein CD3 γ (CD3g) in RNA from purified small bowel IELs (Fig. 1A). Moreover, the level of Glp1r expression in the intestinal IEL compartment was considerably higher than in other mouse primary and secondary lymphoid organs (Fig. 1B). A 1.4-kb product corresponding to the entire coding region of the canonical mouse Glp1r was amplified from RNA obtained from Percoll-purified IELs at levels approximating those detected in RNA from mouse lung (24) (Fig. 1C). Cloning and sequencing of the 1.4-kb IEL Glp1r mRNA transcript demonstrated that it corresponded to the sequence of the canonical murine Glp1r (data not shown).

Figure 1
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Figure 1

A canonical Glp1r transcript is expressed in murine intestinal IELs. A: Glp1r expression copurifies with markers of IELs from mouse small intestine. IELs were isolated from the small intestine and analyzed before (crude IELs) and after enrichment by centrifugation through a discontinuous Percoll gradient (Percoll-purified IELs). mRNA levels are expressed relative to the corresponding jejunal transcript levels and are mean ± SD of three independent IEL preparations. iFabp, intestinal fatty acid binding protein; Clca3, chloride channel calcium activated 3; ChrA, chromogranin A. B: The Glp1r is widely expressed in mouse lymphoid organs but at levels much lower than in intestinal IEL preparations. Glp1r mRNA levels are expressed relative to the Glp1r mRNA content in midjejunum and are mean ± SD. n = 3–6 mice for bone marrow (Bone Marr), thymus, spleen, and mesenteric lymph node (MLN); n = 11 mice for jejunum and colon; n = 6 and 3 independent preparations for small intestine and colon IELs, respectively. C: The mouse Glp1r transcript was identified in RNA isolated from the indicated sources following reverse transcription PCR with primers that amplify a 1.4-kb product encompassing the majority of the Glp1r open reading frame. Southern blotting with an internal Glp1r 32P-labeled oligonucleotide was used to detect the Glp1r PCR product. Lung was used as a positive control for Glp1r expression. Negative controls (-ve controls) correspond to PCR reactions performed in the absence of first-strand cDNA synthesis. Percoll-P, Percoll purified. D: The Glp1r is expressed in both Tαβ and Tγδ IELs from mouse small intestine. Percoll-purified IELs were stained with fluorescently labeled mAbs, and Tαβ and Tδγ were purified by FACS. mRNA levels are expressed relative to jejunal mRNA levels and are mean ± SD from three independent cell-sorting experiments, each involving pooled IELs obtained from five to six mice.

Because Tαβ and Tγδ IELs represent the two major subsets of intestinal IELs, we assessed Glp1r expression in RNA from Percoll-purified and FACS-sorted IEL subsets isolated from the small intestine, revealing abundant Glp1r expression in both IEL subsets (Fig. 1D). Furthermore, we detected a strong correlation between levels of Glp1r mRNA and the relative abundance of transcripts corresponding to the IEL markers Itgae and CD3g along the entire murine small intestine and colon (Supplementary Fig. 1B). Taken together, these findings identify the IEL as a previously unrecognized site of Glp1r expression in the small and large bowel.

Neither Loss of GLP-1R Signaling nor Chronic GLP-1R Agonist Treatment of WT Mice Alters Intestinal IEL Number or Subset Composition

To determine whether the presence or absence of GLP-1R signaling alters IEL homeostasis, we compared the frequency of IELs in the small intestine and colon of Glp1r−/− versus Glp1r+/+ mice and in C57BL/6 mice treated with liraglutide or saline for 1 week (Fig. 2). No significant differences in the phenotypic composition of the IEL population were detected in the small intestine or colon of Glp1r−/− versus Glp1r+/+ littermate control mice (Fig. 2A). Furthermore, loss of GLP-1R signaling did not affect IEL density in the jejunum (Fig. 2B). Likewise, liraglutide had no impact on IEL density in the small intestine (Fig. 2C). Thus, GLP-1R signaling is not critical for IEL development or recruitment to the intestinal niche.

Figure 2
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Figure 2

Loss of GLP-1R signaling does not alter the phenotypic composition of the mouse intestinal IEL population. A: IELs isolated from small intestine (SI) and colon were stained with fluorescently labeled mAbs and analyzed by flow cytometry. Left panels indicate the frequency expressed relative to CD45+ live events within the lymphocyte gate. Right panels are frequencies of the various subpopulations of Tαβ and Tγδ IELs expressed relative to the total number of Tαβ and Tγδ cells, respectively. Data are mean ± SD (small intestine: n = 6 Glp1r+/+ and 9 Glp1r−/− mice; colon: n = 3 mice of each genotype). DN, CD4−CD8− double-negative T cells. B and C: Neither loss of GLP-1R signaling nor chronic treatment with liraglutide affected IEL density in the mouse jejunum. Jejunal IEL number (top panels) and villus height (bottom panels) in Glp1r−/− and Glp1r+/+ mice (B) and in C57BL/6 mice following administration of the GLP-1R agonist liraglutide (75 μg/kg) or PBS vehicle (Veh) twice a day for 7 days (C). Jejunum tissue sections were stained with anti-CD3 antibody and the number of IELs per half-villus and the villus length scored in a blinded manner. Each data point corresponds to one mouse. n = 7–13 mice/group. Data in C are combined from two independent experiments.

Activation of the GLP-1R on Murine Intestinal IELs Suppresses Expression of Inflammatory Cytokines

To assess whether the IEL Glp1r mRNA transcript encodes a functional GLP-1R protein, we measured levels of cAMP in sorted nonactivated IELs in response to Ex-4 (Fig. 3A and B). Ex-4 directly increased cAMP levels in a dose-dependent manner (half-maximal effective concentration 0.35 nmol/L). Furthermore, the relative extent of Ex-4–induced cAMP production was preserved in activated IELs and was comparable in magnitude to the cAMP response triggered by 10 μmol/L Fk (Fig. 3B). Surprisingly, IELs exhibited a modest cAMP response to the adenylyl cyclase activator Fk compared with T cells from the thymus (Supplementary Fig. 1C). Because IELs produce a broad spectrum of cytokines that play key roles in intestinal inflammation, pathogen clearance, and epithelial barrier function (25), we examined whether GLP-1R signaling modified cytokine expression in sorted intestinal IELs in vitro. As a control, we examined splenocytes that express low levels of Glp1r mRNA. Following activation by immobilized anti-CD3 and soluble anti-CD28 antibodies, transcript levels of the proinflammatory cytokines IL-2, IL-17a, interferon γ, and tumor necrosis factor-α robustly increased in isolated IELs and splenocytes (Fig. 3C). Ex-4 significantly attenuated the induction of both mRNA expression and protein production of proinflammatory cytokines in IELs but not in splenocytes (Fig. 3C and Supplementary Fig. 1E). Notably, the magnitude of reduction in cytokine expression in IELs after Ex-4 treatment paralleled reductions observed with Fk (Fig. 3C). The actions of Ex-4 to suppress cytokine induction required the canonical GLP-1R because they were not detected in IELs from Glp1r−/− mice (Fig. 3D). Moreover, the GLP-1R antagonist exendin (9-39) attenuated the Ex-4–dependent suppression of cytokine mRNA expression (Supplementary Fig. 1D). In contrast, Fk robustly inhibited cytokine upregulation in IELs from Glp1r−/− mice (Fig. 3D). These findings link direct activation of GLP-1R signaling to attenuation of proinflammatory cytokine expression in IELs.

Figure 3
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Figure 3

Murine intestinal IELs express a functional GLP-1R. A: Ex-4 stimulates cAMP accumulation in nonactivated IELs from Glp1r+/+ mouse small intestine. Levels of cAMP were quantified in sorted untouched IELs following incubation with increasing concentrations of Ex-4. Prostaglandin E2 (PGE2) (5 μmol/L) was used as a positive control. Shown are combined data (mean ± SD) of two independent batches of sorted cells assayed in triplicate. B: Ex-4 and Fk 10 μmol/L induce comparable increases in cAMP in both activated and nonactivated IELs. Sorted untouched IELs were incubated for 5 h without (nonactivated IELs) or with (activated IELs) anti-CD3/CD28 antibodies. The cells were then challenged with Ex-4, Fk, or vehicle alone (Veh) for 15 min, and cAMP content was quantified. Data are relative to the cAMP levels in Veh-treated nonactivated IELs and are mean ± SD of four independent experiments with two or three replicates each. C and D: Ex-4 suppresses the induction of cytokine expression in activated IELs from Glp1r+/+ (C) but not Glp1r−/− (D) mouse small intestine. Splenocytes and sorted untouched IELs were activated with anti-CD3/CD28 for 5 h in the presence of Ex-4, Fk, or Veh. mRNA levels of the indicated cytokines were assessed by qPCR. Data in C are mean ± SD of one out of five independent experiments with similar results assayed in duplicate or triplicate. Data in D are from a single experiment assayed in triplicate.

The Severity of Intestinal Injury Is Increased in Glp1r−/−Mice

IELs play an important role in maintaining the integrity of the epithelial barrier (15). Baseline small intestinal permeability assessed following 14C-mannitol oral gavage was not different in Glp1r+/+ versus Glp1r−/− mice (Supplementary Fig. 3A). To evaluate the importance of Glp1r in the setting of mucosal injury, we compared the severity of DSS-induced colitis in Glp1r−/− versus Glp1r+/+ mice. Glp1r−/− mice lost significantly more weight despite similar intake of DSS (Fig. 4A and B); colon lengths were shorter at baseline yet reduced to a comparable extent after DSS (Fig. 4C). Furthermore, Glp1r−/− mice exhibited significantly increased disease activity scores (Fig. 4D) and greater epithelial damage (Fig. 4E) reflected by a greater colon damage score (Fig. 4F).

Figure 4
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Figure 4

The severity of DSS-induced colitis is increased in Glp1r−/− mice. Glp1r+/+ or Glp1r−/− mice were maintained on either regular drinking water (Veh) or water supplemented with 3% DSS for 7 days. Body weight and water intake were measured daily for the duration of the treatment. Change in body weight (A), daily water intake (B), colon length (C), disease activity index (DAI) (D), colon histology (E), and colon damage score (F) were recorded. For A–F, n = 9–12 mice/group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Glp1r+/+ (A, C, and F). *P < 0.05 vs. Glp1r+/+ Veh; #P < 0.05 vs. Glp1r+/+ DSS (D).

Intestinal GLP-1R Signaling Modulates the Expression of Genes Involved in Immune Regulation and Epithelial Protection and Repair

To understand how GLP-1R signaling controls mucosal integrity, we examined expression of genes important for epithelial repair, barrier function, and immune regulation in the colon of Glp1r−/− and Glp1r+/+ mice in the presence or absence of DSS-induced colitis (Fig. 5 and Supplementary Fig. 2). Remarkably, even in the absence of experimental gut injury, Glp1r−/− mice exhibited significant reductions in expression of genes that contribute to epithelial protection and repair (trefoil factor [Tff]-1 and -2, transforming growth factor [Tgf]-b1 and -3, epidermal growth factor receptor [Egfr], keratinocyte growth factor [Fgf7], hepatocyte growth factor [Hgf]), the innate immune response (Il6, Il1b), and inflammation (Il12b). Furthermore, induction of gut injury with DSS produced differential colonic gene expression (Tff3, Tgfb2, Ifng, Hgf) in Glp1r+/+ versus Glp1r−/− mice. These findings illustrate that GLP-1R signaling regulates genes important for intestinal homeostasis in the presence and absence of gut inflammation.

Figure 5
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Figure 5

GLP-1R signaling modulates the colonic expression of genes important for immune regulation and epithelial protection and repair. Gene expression was examined in colonic RNA samples from Glp1r+/+ (WT) or Glp1r−/− (knockout [KO]) mice (n = 8–12 mice/group) maintained on regular drinking water or drinking water supplemented with 3% DSS for 7 days. *P < 0.05, **P < 0.01, ***P < 0.001 for Glp1r+/+ vs. Glp1r−/− on regular drinking water; #P < 0.05, ##P < 0.01, ###P < 0.001 for Glp1r+/+ vs. Glp1r−/− on DSS drinking water.

Bone Marrow Radiation Chimeras Establish the Essential Role of the IEL GLP-1R for Control of Intestinal Gene Expression

Because dysregulated intestinal gene expression in Glp1r−/− mice potentially reflects loss of GLP-1R signaling in intestinal and extraintestinal cell types, we reassessed colonic gene expression after transplantation of bone marrow from C57BL/6 male donor mice into Glp1r−/− or Glp1r+/+ recipient females. In such a setting, Glp1r−/− mice are reconstituted with Glp1r+/+ hematopoietic stem cells, including IELs. Chimerism, assessed in spleen DNA from the recipient mice by qPCR, was 84.3 ± 6.4%. Analysis of Glp1r expression in jejunum and sorted IELs from Glp1r−/− and Glp1r+/+ recipient females demonstrated that IELs derived from transplanted marrow repopulated the intestinal compartment (Supplementary Fig. 3B). The reconstitution efficiency of Glp1r expression in the jejunum and IELs of Glp1r−/− recipients 12 weeks after bone marrow transplantation was 64% and 73%, respectively. We focused our analysis on genes shown in Fig. 5 to be dysregulated in the colon of Glp1r−/− mice. Selective re-establishment of WT IELs in the intestinal mucosa of Glp1r−/− mice normalized gene expression profiles for the majority of genes examined (Fig. 6).

Figure 6
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Figure 6

Normalization of colonic gene expression in Glp1r−/− mice after bone marrow transplantation. Following whole-body irradiation and bone marrow transplantation from C57BL/6 donor male mice, gene expression was examined in colonic RNA samples from recipient Glp1r+/+ or Glp1r−/− female mice (n = 4–10 mice/group). *P < 0.05 vs. Glp1r+/+.

Acute Ex-4 Administration Attenuates Edema and Increases Expression of Immunomodulatory and Antimicrobial Genes in the Small Intestine

IELs control intestinal barrier function and repair and protect the intestinal mucosal epithelium by promoting pathogen clearance and lysing pathogen-infected cells (26). Accordingly, we examined expression of immunomodulatory genes in the jejunum of C57BL/6 mice in response to Ex-4 administration (Fig. 7A). Ex-4 markedly upregulated levels of Il1b, Il6, Il22, Il12b, Tnfa, Ccl2, Cxcl1, and Cxcl2 mRNA transcripts. The augmentation of gene expression by Ex-4 was rapid, detectable by 4 h, and transient, with levels of most mRNA transcripts returning to normal by 24 h. Increases in IL-6 and tumor necrosis factor-α protein levels were also observed 4 h after Ex-4 administration (Supplementary Fig. 3C). Ex-4 also significantly increased expression of genes that 1) encode antimicrobial proteins (regenerating islet-derived protein 3 [RegIII]γ and RegIIIβ) or 2) play a role in pathogen clearance (IL-5, IL-13). For some genes, the Ex-4–dependent induction of this group of genes, particularly RegIIIg and RegIIIb, was sustained with continued Ex-4 treatment (Fig. 7A). These data suggest that activation of GLP-1R signaling establishes a generalized cytoprotective response in the murine intestine.

Figure 7
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Figure 7

Acute GLP-1R agonist treatment increases the expression of immunomodulatory and antimicrobial genes in the murine small intestine. A: C57BL/6 mice were treated with Ex-4 (10 nmol/kg) or PBS every 12 h for up to 48 h. Mice were euthanized at 4, 24, and 48 h after the last injection. n = 5 mice/group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. PBS at the same time point. NS, not significant. B: Body weight, colon length, colon weight, and colon weight per unit length. C: Ileal levels of selected mRNA in C57BL/6 mice maintained on drinking water supplemented with 3% DSS for 4 days followed by two injections (12 h apart) of Ex-4 (10 nmol/kg) or PBS. n = 5–6 mice/group. *P < 0.05, **P < 0.01 vs. PBS.

Assessing the effects of Ex-4 or liraglutide in mice with DSS-induced colitis was complicated by marked GLP-1R agonist–mediated reductions in water and, hence, DSS intake, which attenuated intestinal injury. Consequently, we first induced intestinal injury with DSS for 4 days and then treated mice with two injections of Ex-4 12 h apart. Acute Ex-4 had no effect on body weight, colon length, or colon damage score (Fig. 7B and Supplementary Fig. 3D) but significantly reduced colon weight (Fig. 7B) consistent with a reduction of DSS-induced colitis-associated edema. Ex-4 significantly upregulated expression of inflammatory, antimicrobial, and growth factor genes in the ileum (Fig. 7C). Because IELs play important roles in host mucosal defense and response to microbial pathogens, we examined the population of major microbial species by analysis of 16S bacterial rRNA genes in the feces of Glp1r−/− versus Glp1r+/+ mice (Fig. 8A). Remarkably, highly significant differences in the relative abundance of Gammaproteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were detected. Bacteroidetes and Firmicutes represented the majority of the bacterial population in both Glp1r−/− and Glp1r+/+ mice (Supplementary Fig. 4A). Less similarity of microbial profiles was seen between groups than within groups. Taken together, these findings suggest that GLP-1R signaling influences the establishment of the gut microbiome and the normal host immune response to intestinal injury.

Figure 8
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Figure 8

A: Glp1r+/+ and Glp1r−/− mice exhibit significant differences in the relative abundance of several bacterial phyla. Comparisons were made using fecal samples from 11–14-week-old Glp1r+/+ and Glp1r−/− female mice maintained on standard rodent chow and housed in groups of the same genotype per cage (n = 3–5 mice/group). *P < 0.05 for Glp1r+/+ vs. Glp1r−/− mice. B: IELs and L cells define a local cellular network within the boundaries of the intestinal mucosal epithelium that translates information from the gut lumen (nutrients, bacterial products) through GLP-1 to control host mucosal immune responses through modulation of innate immunity.

Discussion

GLP-1R is expressed in the small intestine and colon (23,27); however, previous efforts to identify cellular sites of GLP-1R expression within the gut yielded conflicting results. Using a GLP-1R antibody and immunohistochemistry, Kedees et al. (28) reported GLP-1R expression in the intestinal mucosa, enteric nervous system, and Paneth cells, whereas studies using the GLP-1R promoter to direct expression of a fluorescent reporter protein localized intestinal GLP-1R expression to enteric neurons (23). GLP-1Rs have also been localized to gastric parietal cells in rats (29) and in the Brunner’s gland of the duodenum, parietal cells and smooth muscle cells in the gastric muscularis externa, and myenteric plexus neurons throughout the primate small and large bowel (30). Given the lack of specificity of the majority of commercially available GLP-1R antibodies and existing discrepancies in studies reporting sites of intestinal GLP-1R expression, we initiated studies to localize the sites of endogenous GLP-1R mRNA expression in the murine gut.

Although mRNA transcripts encoding a functional GLP-1R were detected in rodent immune cells isolated from spleen, lymph nodes, and bone marrow (19), the current experiments reveal that IEL Glp1r expression is considerably more abundant relative to Glp1r mRNA levels in other mouse lymphoid organs. Several lines of evidence support the functional importance of the newly described IEL GLP-1R. First, Ex-4 directly increased cAMP accumulation in isolated IELs. Second, Ex-4 robustly suppressed expression of cytokines following activation of IELs from WT mice but had no effect on IELs from Glp1r−/− mice. Furthermore, reconstitution of Glp1r−/− mice with bone marrow from C57BL/6 donors normalized the expression of immune-related genes in the intestine. Taken together, these findings establish the functional importance of the intestinal IEL GLP-1R.

The delineation of an enteroendocrine-immune axis is not without precedent. Infection of severe combined immunodeficient mice with Trichuris muris reduced the number of enteroendocrine cells compared with WT controls, whereas the number of gut endocrine cells increased significantly following reconstitution of immunodeficient mice with CD4+ T cells from infected WT mice (31). Furthermore, bacterial-derived products such as LPS and flagellin independently modulated expression of genes encoding peptide hormones and multiple ligands and receptors important for the immune response in LCC-18 enteroendocrine cells (32). Conversely, receptors for vasoactive intestinal peptide and pituitary adenylate cyclase–activating peptide have been localized to intestinal IELs (33), whereas Toll-like receptors are expressed on enteroendocrine cells, and Toll-like receptor activation by bacterial products promotes secretion of cholecystokinin from gut endocrine cells (34).

Considerable evidence attests to the importance of inflammation and innate immunity in the control of metabolism (35,36); however, few studies have examined whether IELs play a role in the development and/or treatment of experimental models of type 2 diabetes or obesity. Emerging data link cytokine and chemokine activity with control of enteroendocrine cell responses. Direct administration of RANTES (regulated on activation, normal T cell expressed and secreted) to NCIH716 cells rapidly reduces GLP-1 secretion and cAMP accumulation, and RANTES reduces plasma levels of GLP-1 and blunts the rise in insulin levels after glucose administration in vivo (37). In contrast, IL-6, IL-1, and LPS all increase GLP-1 levels in mice (8–10), with IL-6 acting directly on gut endocrine cells to promote GLP-1 secretion. Of note, central blockade of GLP-1R signaling enhanced the pyrogenic effect of LPS (38), implying that endogenous GLP-1 exerts anti-inflammatory actions. Indeed, pharmacological administration of GLP-1R agonists has exerted anti-inflammatory actions in multiple studies through incompletely understood mechanisms (39). The current data extend previous observations by demonstrating that GLP-1 rapidly suppresses a proinflammatory cytokine program from activated intestinal IELs ex vivo, actions requiring a functional GLP-1R. Hence, induction of GLP-1 secretion following administration of cytokines or endotoxin has relevance beyond glucose control and leads to attenuation of the inflammatory response (Fig. 8B).

Previous studies examining the relationship between proglucagon-derived peptides and control of gut inflammation have focused on the related L-cell peptide GLP-2, which potently suppresses inflammation in experimental models of gut injury (40–42). Conversely, disruption of GLP-2R signaling was associated with increased bacterial colonization of the small bowel, reduced bactericidal activity, impaired Paneth cell responses (43), and enhanced susceptibility to gut injury and inflammation (43). The anti-inflammatory actions of GLP-2 are indirect, mediated through reduction of gut permeability (44,45). Indeed, modulation (both gain and loss) of GLP-2R signaling robustly modifies gut barrier function and the extent of inflammation in murine models of diabetes characterized by gut injury and endotoxemia (46). The current findings demonstrate that loss of GLP-1R signaling also enhances the susceptibility to gut injury, albeit through different mechanisms, involving the IEL-GLP-1R signaling system.

Germ-free mice exhibit increased levels of circulating GLP-1 and enhanced proglucagon expression in the murine brainstem (47) and distal gut (48), whereas microbial metabolites such as indole directly activate GLP-1 secretion from L cells (49). Collectively, these emerging findings link the gut microbiome and its metabolic by-products with the control of central and peripheral GLP-1 production. Reduction of energy intake and malnutrition has been associated with defective immune responses, and accumulating evidence supports an intimate link among energy availability, nutrient sensing, and the regulation of the innate immune response (50). Of note, Glp1r−/− mice exhibit resistance to diet-induced obesity (51), increased relative abundance of Bacteroidetes, and reduced abundance of Firmicutes in their microbiota, whereas obese mice show a decrease in Bacteroidetes and an increase in Firmicutes (52). Collectively, the data establish a previously unrecognized local GLP-1-IEL-GLP-1R axis that may influence microbiome composition and the response to intestinal inflammation (Fig. 8B). Because considerable evidence supports a role for gut-derived inflammation in the pathophysiology of insulin resistance arising in subjects with obesity and diabetes (35), we hypothesize that GLP-1R agonists exert favorable nonglycemic actions in part through modulation of IEL-GLP-1R signaling. This hypothesis requires further validation through experimental models of metabolic dysfunction and inflammation.

Article Information

Funding. P.M.S. holds operating grants for the Canadian Institutes of Health Research (MOP-89894 and IOP-92890) and is the recipient of a Canada Research Chair in Gastrointestinal Disease. D.J.D. was supported in part by a Canada Research Chair in Regulatory Peptides, a Banting & Best Diabetes Centre-Novo Nordisk Chair in Incretin Biology, and operating grant 123391 from the Canadian Institutes of Health Research.

Duality of Interest. D.J.D. received partial grant support from Novo Nordisk. D.J.D. has served as an advisor or consultant or speaker within the past 12 months for Arisaph Pharmaceuticals, Inc., Intarcia Therapeutics, Merck Research Laboratories, MedImmune, Novo Nordisk, NPS Pharmaceuticals, Inc., Receptos, Inc., and Sanofi. Mount Sinai Hospital receives partial operating grant support for other studies in the Drucker laboratory from GlaxoSmithKline, Merck, Novo Nordisk, and Sanofi. Neither D.J.D. nor his family members hold stock directly or indirectly in any of these companies. No other potential conflicts of interest relevant to this article were reported.

Author Contributions. B.Y., L.L.B., J.K., D.H., X.C., L.J.P., K.C.J.-H., W.Y., and K.W.A.B. contributed to the planning and carrying out of experiments, analysis of results, and writing and review of the manuscript. M.G.S., P.M.S., and D.J.D. contributed to the planning of experiments, analysis of results, and writing and review of the manuscript. D.J.D. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Footnotes

  • B.Y. and L.L.B. are co-first authors.

  • This article contains Supplementary Data online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db14-1577/-/DC1.

  • See accompanying article, p. 2329.

  • Received October 14, 2014.
  • Accepted February 17, 2015.
  • © 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.

References

  1. ↵
    1. Drucker DJ
    . The role of gut hormones in glucose homeostasis. J Clin Invest 2007;117:24–32pmid:17200703
    OpenUrlCrossRefPubMedWeb of Science
  2. ↵
    1. Xiao Q,
    2. Boushey RP,
    3. Cino M,
    4. Drucker DJ,
    5. Brubaker PL
    . Circulating levels of glucagon-like peptide-2 in human subjects with inflammatory bowel disease. Am J Physiol Regul Integr Comp Physiol 2000;278:R1057–R1063pmid:10749795
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Eissele R,
    2. Göke R,
    3. Willemer S, et al
    . Glucagon-like peptide-1 cells in the gastrointestinal tract and pancreas of rat, pig and man. Eur J Clin Invest 1992;22:283–291pmid:1499644
    OpenUrlCrossRefPubMedWeb of Science
  4. ↵
    1. Campbell JE,
    2. Drucker DJ
    . Pharmacology, physiology, and mechanisms of incretin hormone action. Cell Metab 2013;17:819–837pmid:23684623
    OpenUrlCrossRefPubMedWeb of Science
  5. ↵
    1. Drucker DJ,
    2. Nauck MA
    . The incretin system: glucagon-like peptide-1 receptor agonists and dipeptidyl peptidase-4 inhibitors in type 2 diabetes. Lancet 2006;368:1696–1705pmid:17098089
    OpenUrlCrossRefPubMedWeb of Science
  6. ↵
    1. Drucker DJ,
    2. Yusta B
    . Physiology and pharmacology of the enteroendocrine hormone glucagon-like peptide-2. Annu Rev Physiol 2014;76:561–583pmid:24161075
    OpenUrlCrossRefPubMedWeb of Science
  7. ↵
    1. Nauck MA
    . Is glucagon-like peptide 1 an incretin hormone? Diabetologia 1999;42:373–379pmid:10096792
    OpenUrlCrossRefPubMedWeb of Science
  8. ↵
    1. Ellingsgaard H,
    2. Hauselmann I,
    3. Schuler B, et al
    . Interleukin-6 enhances insulin secretion by increasing glucagon-like peptide-1 secretion from L cells and alpha cells. Nat Med 2011;17:1481–1489pmid:22037645
    OpenUrlCrossRefPubMed
  9. ↵
    1. Nguyen AT,
    2. Mandard S,
    3. Dray C, et al
    . Lipopolysaccharides-mediated increase in glucose-stimulated insulin secretion: involvement of the GLP-1 pathway. Diabetes 2014;63:471–482pmid:24186868
    OpenUrlAbstract/FREE Full Text
  10. ↵
    1. Kahles F,
    2. Meyer C,
    3. Möllmann J, et al
    . GLP-1 secretion is increased by inflammatory stimuli in an IL-6-dependent manner, leading to hyperinsulinemia and blood glucose lowering. Diabetes 2014;63:3221–3229pmid:24947356
    OpenUrlAbstract/FREE Full Text
  11. ↵
    1. Cheroutre H,
    2. Lambolez F,
    3. Mucida D
    . The light and dark sides of intestinal intraepithelial lymphocytes. Nat Rev Immunol 2011;11:445–456pmid:21681197
    OpenUrlCrossRefPubMed
  12. ↵
    1. Hansotia T,
    2. Baggio LL,
    3. Delmeire D, et al
    . Double incretin receptor knockout (DIRKO) mice reveal an essential role for the enteroinsular axis in transducing the glucoregulatory actions of DPP-IV inhibitors. Diabetes 2004;53:1326–1335pmid:15111503
    OpenUrlAbstract/FREE Full Text
  13. ↵
    Lefrançois L, Lycke N. Isolation of mouse small intestinal intraepithelial lymphocytes, Peyer’s patch, and lamina propria cells. Curr Protoc Immunol 2001;4:3.19.1–3.19.16
  14. ↵
    1. Leung FW,
    2. Miller JC,
    3. Reedy TJ,
    4. Guth PH
    . Exogenous prostaglandin protects against acid-induced deep mucosal injury by stimulating alkaline secretion in rat duodenum. Dig Dis Sci 1989;34:1686–1691pmid:2582981
    OpenUrlCrossRefPubMedWeb of Science
  15. ↵
    1. Chen Y,
    2. Chou K,
    3. Fuchs E,
    4. Havran WL,
    5. Boismenu R
    . Protection of the intestinal mucosa by intraepithelial gamma delta T cells. Proc Natl Acad Sci U S A 2002;99:14338–14343pmid:12376619
    OpenUrlAbstract/FREE Full Text
  16. ↵
    1. Panjwani N,
    2. Mulvihill EE,
    3. Longuet C, et al
    . GLP-1 receptor activation indirectly reduces hepatic lipid accumulation but does not attenuate development of atherosclerosis in diabetic male ApoE(-/-) mice. Endocrinology 2013;154:127–139pmid:23183176
    OpenUrlCrossRefPubMedWeb of Science
  17. ↵
    Auletta JJ, Devecchio JL, Ferrara JL, Heinzel FP. Distinct phases in recovery of reconstituted innate cellular-mediated immunity after murine syngeneic bone marrow transplantation. Biol Blood Marrow Transplant 2004;10:834–847
  18. ↵
    1. Chomczynski P,
    2. Sacchi N
    . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156–159pmid:2440339
    OpenUrlCrossRefPubMedWeb of Science
  19. ↵
    1. Hadjiyanni I,
    2. Siminovitch KA,
    3. Danska JS,
    4. Drucker DJ
    . Glucagon-like peptide-1 receptor signalling selectively regulates murine lymphocyte proliferation and maintenance of peripheral regulatory T cells. Diabetologia 2010;53:730–740pmid:20225396
    OpenUrlCrossRefPubMedWeb of Science
  20. ↵
    1. Assa A,
    2. Vong L,
    3. Pinnell LJ, et al
    . Vitamin D deficiency predisposes to adherent-invasive Escherichia coli-induced barrier dysfunction and experimental colonic injury. Inflamm Bowel Dis 2015;21:297–306pmid:25590952
    OpenUrlCrossRefPubMed
  21. ↵
    1. Huson DH,
    2. Mitra S,
    3. Ruscheweyh HJ,
    4. Weber N,
    5. Schuster SC
    . Integrative analysis of environmental sequences using MEGAN4. Genome Res 2011;21:1552–1560pmid:21690186
    OpenUrlAbstract/FREE Full Text
  22. ↵
    1. Pyke C,
    2. Knudsen LB
    . The glucagon-like peptide-1 receptor—or not? Endocrinology 2013;154:4–8pmid:23267050
    OpenUrlCrossRefPubMedWeb of Science
  23. ↵
    1. Richards P,
    2. Parker HE,
    3. Adriaenssens AE, et al
    . Identification and characterisation of glucagon-like peptide-1 receptor expressing cells using a new transgenic mouse model. Diabetes 2014;63:1224–1233pmid:24296712
    OpenUrlAbstract/FREE Full Text
  24. ↵
    1. Bullock BP,
    2. Heller RS,
    3. Habener JF
    . Tissue distribution of messenger ribonucleic acid encoding the rat glucagon-like peptide-1 receptor. Endocrinology 1996;137:2968–2978pmid:8770921
    OpenUrlCrossRefPubMedWeb of Science
  25. ↵
    Qiu Y, Yang H. Effects of intraepithelial lymphocyte-derived cytokines on intestinal mucosal barrier function. J Interferon Cytokine Res 2013;33:551–562
  26. ↵
    1. Kronenberg M,
    2. Havran WL
    . Frontline T cells: gammadelta T cells and intraepithelial lymphocytes. Immunol Rev 2007;215:5–7pmid:17291274
    OpenUrlCrossRefPubMedWeb of Science
  27. ↵
    1. Campos RV,
    2. Lee YC,
    3. Drucker DJ
    . Divergent tissue-specific and developmental expression of receptors for glucagon and glucagon-like peptide-1 in the mouse. Endocrinology 1994;134:2156–2164pmid:8156917
    OpenUrlCrossRefPubMedWeb of Science
  28. ↵
    1. Kedees MH,
    2. Guz Y,
    3. Grigoryan M,
    4. Teitelman G
    . Functional activity of murine intestinal mucosal cells is regulated by the glucagon-like peptide-1 receptor. Peptides 2013;48:36–44pmid:23927844
    OpenUrlCrossRefPubMedWeb of Science
  29. ↵
    1. Schmidtler J,
    2. Dehne K,
    3. Allescher H-D, et al
    . Rat parietal cell receptors for GLP-1-(7-36) amide: northern blot, cross-linking, and radioligand binding. Am J Physiol 1994;267:G423–G432pmid:7943240
    OpenUrlPubMed
  30. ↵
    1. Pyke C,
    2. Heller RS,
    3. Kirk RK, et al
    . GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody. Endocrinology 2014;155:1280–1290pmid:24467746
    OpenUrlCrossRefPubMedWeb of Science
  31. ↵
    1. Wang H,
    2. Steeds J,
    3. Motomura Y, et al
    . CD4+ T cell-mediated immunological control of enterochromaffin cell hyperplasia and 5-hydroxytryptamine production in enteric infection. Gut 2007;56:949–957pmid:17303597
    OpenUrlAbstract/FREE Full Text
  32. ↵
    1. Selleri S,
    2. Palazzo M,
    3. Deola S, et al
    . Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS. Int Immunol 2008;20:961–970pmid:18544573
    OpenUrlAbstract/FREE Full Text
  33. ↵
    1. Qian BF,
    2. Hammarström ML,
    3. Danielsson A
    . Differential expression of vasoactive intestinal polypeptide receptor 1 and 2 mRNA in murine intestinal T lymphocyte subtypes. J Neuroendocrinol 2001;13:818–825pmid:11578532
    OpenUrlCrossRefPubMed
  34. ↵
    1. Bogunovic M,
    2. Davé SH,
    3. Tilstra JS, et al
    . Enteroendocrine cells express functional Toll-like receptors. Am J Physiol Gastrointest Liver Physiol 2007;292:G1770–G1783pmid:17395901
    OpenUrlAbstract/FREE Full Text
  35. ↵
    1. Jin C,
    2. Henao-Mejia J,
    3. Flavell RA
    . Innate immune receptors: key regulators of metabolic disease progression. Cell Metab 2013;17:873–882pmid:23747246
    OpenUrlCrossRefPubMedWeb of Science
  36. ↵
    1. Hotamisligil GS
    . Inflammation and metabolic disorders. Nature 2006;444:860–867pmid:17167474
    OpenUrlCrossRefPubMedWeb of Science
  37. ↵
    1. Pais R,
    2. Zietek T,
    3. Hauner H,
    4. Daniel H,
    5. Skurk T
    . RANTES (CCL5) reduces glucose-dependent secretion of glucagon-like peptides 1 and 2 and impairs glucose-induced insulin secretion in mice. Am J Physiol Gastrointest Liver Physiol 2014;307:G330–G337pmid:24875103
    OpenUrlAbstract/FREE Full Text
  38. ↵
    Rinaman L, Comer J. Antagonism of central glucagon-like peptide-1 receptors enhances lipopolysaccharide-induced fever. Auton Neurosci 2000;85:98–101
  39. ↵
    1. Drucker DJ,
    2. Rosen CF
    . Glucagon-like peptide-1 (GLP-1) receptor agonists, obesity and psoriasis: diabetes meets dermatology. Diabetologia 2011;54:2741–2744pmid:21892687
    OpenUrlCrossRefPubMed
  40. ↵
    1. Boushey RP,
    2. Yusta B,
    3. Drucker DJ
    . Glucagon-like peptide (GLP)-2 reduces chemotherapy-associated mortality and enhances cell survival in cells expressing a transfected GLP-2 receptor. Cancer Res 2001;61:687–693pmid:11212269
    OpenUrlAbstract/FREE Full Text
    1. Drucker DJ,
    2. Yusta B,
    3. Boushey RP,
    4. DeForest L,
    5. Brubaker PL
    . Human [Gly2]GLP-2 reduces the severity of colonic injury in a murine model of experimental colitis. Am J Physiol 1999;276:G79–G91pmid:9886982
    OpenUrlPubMedWeb of Science
  41. ↵
    1. Boushey RP,
    2. Yusta B,
    3. Drucker DJ
    . Glucagon-like peptide 2 decreases mortality and reduces the severity of indomethacin-induced murine enteritis. Am J Physiol 1999;277:E937–E947pmid:10567023
    OpenUrlPubMed
  42. ↵
    1. Lee S-J,
    2. Lee J,
    3. Li KK, et al
    . Disruption of the murine Glp2r impairs Paneth cell function and increases susceptibility to small bowel enteritis. Endocrinology 2012;153:1141–1151pmid:22253424
    OpenUrlCrossRefPubMedWeb of Science
  43. ↵
    1. Hadjiyanni I,
    2. Li KK,
    3. Drucker DJ
    . Glucagon-like peptide-2 reduces intestinal permeability but does not modify the onset of type 1 diabetes in the nonobese diabetic mouse. Endocrinology 2009;150:592–599pmid:18845625
    OpenUrlCrossRefPubMedWeb of Science
  44. ↵
    1. Bahrami J,
    2. Longuet C,
    3. Baggio LL,
    4. Li K,
    5. Drucker DJ
    . Glucagon-like peptide-2 receptor modulates islet adaptation to metabolic stress in the ob/ob mouse. Gastroenterology 2010;139:857–868pmid:20546737
    OpenUrlCrossRefPubMedWeb of Science
  45. ↵
    1. Cani PD,
    2. Possemiers S,
    3. Van de Wiele T, et al
    . Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut 2009;58:1091–1103pmid:19240062
    OpenUrlAbstract/FREE Full Text
  46. ↵
    1. Schéle E,
    2. Grahnemo L,
    3. Anesten F,
    4. Hallén A,
    5. Bäckhed F,
    6. Jansson JO
    . The gut microbiota reduces leptin sensitivity and the expression of the obesity-suppressing neuropeptides proglucagon (Gcg) and brain-derived neurotrophic factor (Bdnf) in the central nervous system. Endocrinology 2013;154:3643–3651pmid:23892476
    OpenUrlCrossRefPubMed
  47. ↵
    1. Wichmann A,
    2. Allahyar A,
    3. Greiner TU, et al
    . Microbial modulation of energy availability in the colon regulates intestinal transit. Cell Host Microbe 2013;14:582–590pmid:24237703
    OpenUrlCrossRefPubMedWeb of Science
  48. ↵
    1. Chimerel C,
    2. Emery E,
    3. Summers DK,
    4. Keyser U,
    5. Gribble FM,
    6. Reimann F
    . Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells. Cell Reports 2014;9:1202–1208pmid:25456122
    OpenUrlCrossRefPubMed
  49. ↵
    1. Tsalikis J,
    2. Croitoru DO,
    3. Philpott DJ,
    4. Girardin SE
    . Nutrient sensing and metabolic stress pathways in innate immunity. Cell Microbiol 2013;15:1632–1641pmid:23834352
    OpenUrlPubMed
  50. ↵
    1. Hansotia T,
    2. Maida A,
    3. Flock G, et al
    . Extrapancreatic incretin receptors modulate glucose homeostasis, body weight, and energy expenditure. J Clin Invest 2007;117:143–152pmid:17187081
    OpenUrlCrossRefPubMedWeb of Science
  51. ↵
    1. Hartstra AV,
    2. Bouter KE,
    3. Bäckhed F,
    4. Nieuwdorp M
    . Insights into the role of the microbiome in obesity and type 2 diabetes. Diabetes Care 2015;38:159–165pmid:25538312
    OpenUrlAbstract/FREE Full Text
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GLP-1R Agonists Modulate Enteric Immune Responses Through the Intestinal Intraepithelial Lymphocyte GLP-1R
Bernardo Yusta, Laurie L. Baggio, Jacqueline Koehler, Dianne Holland, Xiemin Cao, Lee J. Pinnell, Kathene C. Johnson-Henry, William Yeung, Michael G. Surette, K.W. Annie Bang, Philip M. Sherman, Daniel J. Drucker
Diabetes Jul 2015, 64 (7) 2537-2549; DOI: 10.2337/db14-1577

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GLP-1R Agonists Modulate Enteric Immune Responses Through the Intestinal Intraepithelial Lymphocyte GLP-1R
Bernardo Yusta, Laurie L. Baggio, Jacqueline Koehler, Dianne Holland, Xiemin Cao, Lee J. Pinnell, Kathene C. Johnson-Henry, William Yeung, Michael G. Surette, K.W. Annie Bang, Philip M. Sherman, Daniel J. Drucker
Diabetes Jul 2015, 64 (7) 2537-2549; DOI: 10.2337/db14-1577
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